Fatal intoxication with tianeptine (Stablon)

Tianeptine (Stablon^®), although structurally similar to tricyclic antidepressants, acts by enhancing the reuptake of serotonin. A fatal case is presented involving a 26-year-old man, found lying in bed with a “mushroom of foam” around his mouth. Empty blister packs of Stablon^® and a suicide note were found next to the body. A liquid–liquid extraction procedure with n-hexane: ethyl acetate and n-hexane: 2-propanol, followed by LC-DAD-MS analysis, using positive mode electrospray ionization was performed. The detection limit was 0.001 μg/mL. The toxicological results revealed the following tianeptine concentrations in the post-mortem samples: blood 5.1 μg/mL; urine 2.0 μg/mL; liver 23 μg/g; stomach contents 22 mg. Femoral blood analyses also revealed an ethanol concentration of 0.53 g/L. The present method was also developed and validated for the other post-mortem specimens, since no previous published data had confirmed the post-mortem distribution of tianeptine. The absence of other suitable direct causes of death (macroscopic or histological) and the positive results achieved with the toxicological analysis led the pathologist to rule that death was due to an intoxication caused by the suicidal ingestion of tianeptine in combination with alcohol.     

Depression and substance use in two divergent high school cultures: A quantitative and qualitative analysis

Research has generally concluded that adolescent depression and substance use are strongly interrelated, but has rarely considered how this relationship may vary across diverse populations. In this study, we used quantitative and qualitative methods to explore the relationships among depression and cigarette, alcohol, marijuana, and harder drug use across two culturally disparate environments: a suburban and an inner-city high school. Our sample included 164 suburban and 242 inner-city high school students. The students completed Kovacs' Children's Depression Inventory of 1985 and substance use measures derived from various sources. In-depth semistructured interviews were conducted with subjects who scored in the top 10% of the CDI (N=19) from both schools. Our quantitative findings indicated a positive association between depression and cigarette, marijuana, and harder drug use among the suburban students, and no association between depression and the use of any substances for the urban students. There were no significant differences in levels of reported depression across samples. However, with the exception of marijuana use, suburban students reported greater involvement in substance use than urban students. Our qualitative analyses suggest that across-school differences in the relationships among depression and substance use may be related to the varied meanings of depression and substance use that are informed by cultural context.The research was funded by the National Institute of Drug Abuse (NIDA), Grant No. 1 R01 DA-06844, Perry London, principal investigator. The views, opinions, and findings contained in their article are not to be construed as NIDA's position or policy.B.A. from University of California, Berkeley, and Ed.D. from the Graduate School of Education, Harvard University. Research interests include the phenomenology of high-risk behavior and social development among urban adolescents.B.A. from Mount Holyoke College and Ed.M. from the Graduate School of Education, Harvard University. Research interests include the relationships among high-risk behavior, personality variables, and cultural context.B.A. from Michigan State University, M.A. from University of Minnesota-Duluth, and Ed.D. from Harvard Graduate School of Education. Research interests include integrating quantitative and qualitative methods in social science research and the phenomenology of high-risk behavior.Research interests included adolescent high-risk behavior and ethnic and religious identity development. Deceased June 1992.     

The importance of geopolitics in firms' international location decisions: The Polish case

Despite connections and common traits between geopolitics and International Business based on geography and location, literature on this matter is scarce. This study aims to contribute to this literature gap, by assessing the importance of Poland's geopolitical factors in FDI location decisions. By applying a hybrid methodology which combines qualitative and quantitative analyses we conclude that there is a connection between Poland's geopolitical factors (stable, variable and interactional) and the volume, origin and distribution of the FDI received.     

European Legal Method in Denmark and Sweden—Using Social Science Theory and Methodology to Describe the Implementation of EU Law

This article presents data from a study in which national bureaucrats working in the fields of taxation and food law in Sweden and Denmark are asked which legal sources and methods of interpretation they use when implementing EU law. The purpose is to contribute to the discussion about European legal method by using social science methodology. National agencies and authorities in the fields of taxation and food law face a ‘multilayered’ or ‘multiprincipal’ reality in which there is room for policy choices. The answers given by the interviewees speak of a plurality of legal sources, a situation where bureaucrats are becoming reluctant lawmakers instructing others on how EU law is to be applied and where bureaucrats find it necessary to found their decisions on what colleagues within the authority or from other Member States have said about how EU law should be applied.     

Introduction to Symposium on Keith J. Bybee's All Judges Are Political—Except When They Are Not

This introduction to the symposium on Keith J. Bybee's All Judges Are Political—Except When They Are Not provides a brief synopsis of the book's main argument and identifies some of the unsettled questions generated by the author's innovative treatment of courtesy, hypocrisy, and law.     

Forensic botany: usability of bryophyte material in forensic studies

Two experiments were performed to test the relevance of bryophyte (Plantae, Bryophyta) material for forensic studies. The first experiment was conducted to reveal if, and how well, plant fragments attach to footwear in general. In the test, 16 persons walked outdoors wearing rubber boots or hiking boots. After 24h of use outdoors the boots were carefully cleaned, and all plant fragments were collected. Afterwards, all plant material was examined to identify the species. In the second experiment, fresh material of nine bryophyte species was kept in a shed in adverse conditions for 18 months, after which DNA was extracted and subjected to genotyping to test the quality of the material. Both experiments give support for the usability of bryophyte material in forensic studies. The bryophyte fragments become attached to shoes, where they remain even after the wearer walks on a dry road for several hours. Bryophyte DNA stays intact, allowing DNA profiling after lengthy periods following detachment from the original plant source. Based on these experiments, and considering the fact that many bryophytes are clonal plants, we propose that bryophytes are among the most usable plants to provide botanical evidence for forensic investigations.     

Euthanasia in the new born： doubts and interrogations

The debates on euthanasia are quite common nowadays, but few refer to euthanasia in newborns. But the day-to-day medical activities pose a lot of questions about the medical treatments of premature or newborn infants （with severe malformations which were not detected during pregnancy）. What is the criminal liability of the doctor in case of omission of treatment in newborns with severe birth defects？ The work will focus on an analysis of crimes of abortion, the omission of medical treatment and murder and injuries. At last I will try to identify the main trends to find a solution to the different situations-such as injury of the fetus in utero, the omission of treatment of newborns with severe malformations and the omission of basic health care of infants with minor malformations.     

SNPs in paternity investigation: The simple future

Based on the 52 SNP-plex developed by the SNPforID Consortium, we designed two 10-plex to study single nucleotide polymorphisms (SNPs) for human identification and to establish its usefulness in paternity casework. This 20 autosomal SNP set was studied in 56 paternity investigation cases from South Portuguese resident population, also analyzed with 17 Short Tandem Repeats (STRs). Results obtained with both methodologies were consistent with each other, except for one case where the alleged father could not be excluded by SNPs. No mutation was found in the SNP loci, whereas a mismatch in STRs was detected. The use of SNPs as a complement to the analysis of autosomal STRs in paternity casework can result in paternity index and paternity probability values equivalent or higher than those obtained with more STR loci, but with lower costs. This study shows that instead of using additional STR loci, the analysis of 20 autosomal SNPs, as a complement technique to standard methodologies, is an appealing alternative in paternity investigation cases.     

MtSNP typing before mtDNA sequencing: Why do it?

Analysis of control mitochondrial DNA (mtDNA) hypervariable regions is sometimes the only available method to study hair evidence in forensic casework although being a laborious technique. Nowadays there is a huge interest in new genetic markers such as single nucleotide polymorphisms (SNPs) to type degraded forensic samples. For that purpose, a 10-Plex mitochondrial SNP for haplogroup typing, chosen from several SNP studies and useful to study the most common populations in our laboratory was applied in forensic casework. Hair shafts from three forensic cases with different ethnic backgrounds were studied with mtDNA sequencing and compared with mitochondrial SNPs (mtSNPs) study. Coding mtSNP typing prior to sequencing can allow for a rapid screening in forensic casework, which is emphasized in the first two cases. Moreover, in cases in which mtDNA sequencing fails, mtSNPs can still be detected. This 10 SNP loci multiplex provides a less expensive and simpler method for mitochondrial typing compared to control region mtDNA sequencing, especially when used as a fast screening method.