Spatial position of the cranium in craniofacial establishment of identity

A total of 90 images of male cranes from different groups of horizontal profiling (120 +/- 10 degrees, 140 +/- 10 degrees, 160 +/- 10 degrees) were studied in experiment with the coordinate-controlled complex for turning angle (from the position full face to the right and left by 14 degrees with spacing 2 degrees). Each image was marked according to the system of 18 control points. Precision of the angle fixation was 0.1 degrees. Calculation of the angles of horizontal profiling and turning of the crane were made according to a specially devised program INPUTTING. Statistic processing of the computer data base made it possible to calculate precision rates of marking points and regression equation describing the pattern of cranial control points in turning. It is shown that presicion of the turning angle estimation with reference to horizontal profiling is much higher than without it. Basing on the data obtained a method of zeroing of coordinates of the control points (placing in position for correct scaling of the objects of craniofacial identification) was devised. The method allows greater reliability of the results of personal craniofacial identification.     

Structural alterations in the human brain in chronic alcohol intoxication

Different brain samples were studied in 23 male alcoholics and 14 healthy victims who had died of heart and major vessels wounds to determine histological changes in the brain and quantitative relations between its tissue structures. The complex of histological, stereo- and morphometric examinations has shown that the brain of alcoholics is characterized by sclerosis and hyalinosis of the vascular branches as well as dystrophic, atrophic and hypertrophic changes of neurocytes. Moreover, there were calcinated petrificates and the cysts, foci of demyelination, diffuse microglyosis. These alterations are accompanied with impairment of quantitative correlations between tissue brain structures. The diameter of cerebral capillaries narrows with resultant lessening of vascular bed capacity and chronic brain ischemia. The concentration of nervous cells decreased because of their progressive atrophy and death while the indices of the glyal component development rose. The findings are important for tanatogenesis and postmortem forensic-medical diagnosis of alcoholism.     

Population data for Y-chromosome STR haplotypes from Piedmont (Italy)

Eight Y-chromosome STR loci (DYS19, DYS389-I, DYS389-II, DYS390, DYS391, DYS392, DYS393 and DYS385) were analysed in a sample of 236 unrelated males from four towns of Piedmont (Trino, Biella, Cavaglià, Postua). One hundred and fifty six different haplotypes were observed and 55 of them were not previously observed in the Y-STR Haplotype Reference Database (http://www.ystr.org/).     

The dynamic prediction of antisocial behavior among forensic psychiatric patients: a prospective field study

Staff ratings of 595 supervised forensic psychiatric patients on the Proximal Risk Factor Scale and the Problem Identification Checklist were completed monthly for an average of 33 months. During the follow-up, there were 265 incidents, 86 of which were violent. The average ratings, excluding those from the index month, differentiated patients who had incidents from those who did not. As well, the average ratings distinguished between individuals with and without incidents of a violent or sexual nature. There were significant increases in staff ratings in the months preceding the index incident month. Within-patient analyses showed that changes in dynamic risk scales comprising the best items for predicting incidents of any kind and violent or sexual incidents were strongly related to their respective outcomes and were significantly related to outcome in an independent sample. Changes in monthly staff ratings predict the imminent occurrence of antisocial and violent behaviors.     

Tilting at Offshore Windmills: Regulating Wind Farm Development Within the Renewable Energy Zone

Offshore wind farms are likely to be a key component of theUnited Kingdom’s strategy for renewable energy, and theEnergy Act 2004 introduced a new legislative framework for thedevelopment of wind farms beyond the limits of territorial seawithin designated renewable energy zones. The compatibilityof the new arrangements with international marine and environmentalobligations is analysed. Rights of navigation, the protectionof the marine environment and the decommissioning of turbinesat the end of their operational life all raise significant legalissues. The introduction of the Energy Act is an important developmentin assisting the construction and operation of offshore windfarms. But the law was passed at a time when a number of majorreviews had concluded that the legislative and policy frameworkfor the protection of the marine environment suffered severeweaknesses. It is unfortunate that the UK Government did notconsider the creation of an offshore renewable energy industryas an opportunity to develop and apply the sort of new marineconservation strategies and legal frameworks being advocatedas essential in this area.     

Allele frequencies for 15 autosomal STR loci and admixture estimates in Puerto Rican Americans

Allelic frequencies of 15 short tandem repeats (STR) markers (CSF1PO, FGA, THO1, TPOX, VWA, D3S11358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, D19S433 and D2S1338) were determined using the AmpFl STR Identifiler PCR Amplification Kit in Puerto Rican American individuals (N=205) from Massachusetts. The FGA, D18S51 and D2S1338 loci had a high power of discrimination (PD) with values of 0.967, 0.965 and 0.961, respectively. Significant deviations from the Hardy-Weinberg (HW) equilibrium were not detected. An important genetic contribution of Caucasian European (76.4%) was detected in Puerto Rican Americans. However, comparative analysis between Puerto Rican American and other neighboring populations from United States mainly with African and Caucasian Americans, revealed significant differences in the distribution of STR markers. Our results are important for future comparative genetic studies of different American ethnic groups, in particular a cultural group called Hispanic-Americans and should be helpful for forensic and paternity testing.     

Analytical approach to determine ticlopidine in post-mortem blood

A new and sensitive method to determine ticlopidine in whole human blood using proadifen as the internal standard (IS) is described. The analyte and IS were extracted by solid-phase extraction using Oasis HLB cartridges, and the extracts were analyzed by gas chromatography-electron impact ionisation-mass spectrometry (GC/EI-MS). Calibration curves were established daily in spiked blood samples using a non-linear calibration model, between 0.01 and 4.5 microg/mL. The correlation coefficients were higher than 0.995. Precision and accuracy fulfilled the internationally accepted criteria (coefficients of variation were less than 9%, and the measured concentrations were within +/-7% of the true value). Limits of detection and quantitation were respectively, 3 and 10 ng/mL. No interfering substances were detected by analysis of 10 blank blood samples of different origin. Mean recovery, calculated at three concentration levels, was 71%. Because of its simplicity and speed, the proposed method can be applied in the determination of this inhibitor of platelet aggregation in whole blood samples, and is suitable for application in toxicology routine analysis.     

Forensic evaluation of the QIAshredder/QIAamp DNA extraction procedure

The potential to recover genetic profiles from evidence samples has substantially increased since robust and sensitive amplification kits are commercially available. Nevertheless, even the best amplification kits cannot succeed when the extracted DNA is of poor quality. In this study we compared the efficiency of silica (QIAamp DNA Mini Kit), Chelex and Phenol-Chloroform (PC) based protocols to recover DNA from different categories of samples (blood and saliva on cotton swabs, muscles, cigarette butts, saliva on foods and epidermal cells on clothes). The efficiency of the QIAamp system was improved when samples were treated with QIAshredder homogenizing columns. Overall, conventional Chelex or PC protocols allowed to recover conclusive SGM Plus profiles for 61% of the samples considered in this study. Contrastingly, 82% of them were successfully genotyped after being treated with a combination of QIAshredder and QIAamp systems. Our results further suggested that the QIAshredder/QIAamp protocol was particularly helpful to analyze evidence samples with few DNA and/or that were collected on substrates containing PCR inhibitors.     

Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise

Recently, there has been much debate about what kinds of genetic markers should be implemented as new core loci that constitute national DNA databases. The choices lie between conventional STRs, ranging in size from 100 to 450 bp; mini-STRs, with amplicon sizes less than 200 bp; and single nucleotide polymorphisms (SNPs). There is general agreement by the European DNA Profiling Group (EDNAP) and the European Network of Forensic Science Institutes (ENFSI) that the reason to implement new markers is to increase the chance of amplifying highly degraded DNA rather than to increase the discriminating power of the current techniques. A collaborative study between nine European and US laboratories was organised under the auspices of EDNAP. Each laboratory was supplied with a SNP multiplex kit (Foren-SNPs) provided by the Forensic Science Service, two mini-STR kits provided by the National Institute of Standards and Technology (NIST) and a set of degraded DNA stains (blood and saliva). Laboratories tested all three multiplex kits, along with their own existing DNA profiling technique, on the same sets of degraded samples. Results were collated and analysed and, in general, mini-STR systems were shown to be the most effective. Accordingly, the EDNAP and ENFSI working groups have recommended that existing STR loci are reengineered to provide smaller amplicons, and the adoption of three new European core loci has been agreed.