HLA-DRB1位点的PCR-SSP分型在亲子鉴定中的应用研究

应用PCR－SSP（PCRamplificationwithsequencespecificprimer）方法将HLAⅡ类DRB1位点基因分型应用于亲权鉴定。对42例亲子鉴定案例进行分析研究的结果表明,本方法简单、快速、结果可靠,且具有较高的非父排除概率（66．3％）,不仅适用于法医学亲手鉴定和个人识别,亦可应用于移植配型、HLA相关疾病及人类遗传学研究。     

Study of the high polymorphism STR locus-FGA in Chinese population

Population genetic study of the complicated STR locus-FGA was performed by the method of PCR in Han population samples. A total of 21 different alleles including 7 interalleles and 63 different genotypes were observed in 349 unrelated individuals. The observed genotype discrimination showed no significant deviation from Hardy-Weinberg equilibrium and 51 family studies showed no mutation. According to the results obtained in this study(DP = 0.9612, PE = 0.7007, PIC = 0.8333), this system can be used as an useful means in forensic identification of both criminal and paternity case.     

Polymorphism analysis of D19S253 locus in Guangzhou area population

Frequency data for STR system D19S253 were obtained from 105 unrelated individuals of Guangzhong population. PCR products were detected by horizontal native polyacrylamide gel electrophoresis and a total of 9 alleles were identified by side-by-side comparison with a sequenced allelic ladder prepared by ourselves. The observed genotype distribution conformed with Hardy-Weinberg equilibrium. The high information content found in this system(heterozygosity rate was 0.8353, the mean exclusion chance was 0.7499, the discrimination power was 0.9211, the polymorphism information content was 0.8203) indicated it is a useful means in forensic routine casework both in criminal and paternity cases.     

Cardic death and apoptosis of myocardium

TdT-mediated Dutp nick end labeling (TUNEL) method was applied to detect apoptosis of myocardium of corpses dying from cardiac death. For arteriosclerotic coronary heart death, diffused apoptosis was observed around the border zones of histologically infarcted myocardium, and in the epithelial and smooth muscle of arteries. Few apoptosis cells were present for hypertensive heart death, and none for corpses dying from falling and hanging. Myocardium apoptosis may provide a new target for heart failure.     

Population study of the CSF1PO,TPOX and TH01 STR polymorphisms in east China

Allele and genotype frequencies of four short tandem repeat loci were determined in a population sample from east China by using PCR and fluorescence techniques. After denaturing PAGE, 9 alleles were identified for CSF1PO, 8 alleles for TPOX, and 7 alleles for TH01. All loci met Hardy-Weinberg expectations. In addition, the theoretical values of these loci for paternity and forensic casework were evaluated.     

武汉地区汉族人群CSF1PO座位多态性分析

应用Ｙｏｓｈｉｄａ设计的ＣＳＦ１ＰＯ新引物对武汉地区３１２例汉族个体作了分型调查,获得了武汉地区汉族人ＣＳＦ１ＰＯ座位的基因频率资料。与Ｈａｍｍｏｎｄ等提出的传统引物扩增效果相比较,本文所选用的引物更适用于法医检案,尤其对严重降解ＤＮＡ材料的分型效果有明显的优越性     

未成年女性性认知能力量化的初步研究

探讨未成年女性性认知能力的量化评定方法,编制未成年女性性认知能力问卷,对未成年女性进行初步试测,结果反映在法定奸幼年龄标准以下的未成年女性中,存在因年龄而异的对性的生理卫生知识、社会法律意识、伦理道德等方面认知能力的相应缺乏,结论：此问卷对未成年女性性认知能力的测试结果与她们的年龄之间具有高度相关性,值得作为标准化定量评定方法进行深入研究