Elections,repression and authoritarian survival in post-transition Indonesia and the Philippines

Abstract

This paper compares post-transition Philippines and Indonesia, examining the ways in which authoritarian practices survive and are shaped by regime transition. It examines the transition process in each case, to identify the problems of management and control that regime elites set for themselves in the post-dictatorship period. It is argued that Philippine elites set out to disaggregate and domesticate an already mobilized opposition movement, while the Indonesian authorities strove to keep similar popular politics from mobilizing. The paper then considers how these political objectives find expression in the structuring of two important institutional fields – the electoral and policy making processes – concluding with an examination of how these considerations influence patterns of repression. In particular, the paper also investigates whether repression targets primarily proscribed modes of activity, or sets out to threaten and intimidate proscribed organizations and people. Differences in electoral and policy processes, as well as in patterns of repression, demonstrate the ways in which authoritarianism can survive regime transitions and can undermine the promise of democracy in the post-dictatorship period.     

Optimization of DNA extraction from low-yield and degraded samples using the BioRobot EZ1 and BioRobot M48

Robotic extraction of DNA from dilutions of blood and semen using either the BioRobots EZ1 or BioRobots M48 consistently produced lower recoveries than standard organic extractions of the same samples. In an effort to increase the efficiency of robotically extracted DNA, glycogen and carrier RNA were added following cell lysis. The addition of glycogen, postlysis, resulted in no improvement in DNA recovery with the BioRobot EZ1. However, when carrier RNA was added to the cell lysate of limited and degraded samples extracted on the EZ1 or the M48, DNA recoveries dramatically increased four- to 20-fold. DNA yields obtained by robotic extraction in the presence of carrier RNA were as high, or higher, as those obtained by organic extraction lacking carrier RNA, while experiments that utilized carrier RNA in both types of extractions showed increased sensitivity for both methods. Furthermore, carrier RNA substantially increased the recovery of fragmented DNA with the EZ1.