三种哺乳动物朊蛋白基因的克隆与分析

以外周血液的总DNA为模板,运用PCR方法克隆了汉族人、秦川黄牛和甘肃土种绵羊的Prnp基因,运用分子生物学软件对这些序列进行了分析比较。结果表明,获得的3种哺乳动物的Prnp基因均位于1个单一的外显子内,与GenBank中登录的相应序列具有高度同源性,达99.0%。牛与绵羊Prnp基因同源性为97.3%,推导氨基酸序列同源性为96.5%。人Prnp基因与黄牛和绵羊Prnp基因的同源性分别为86.7%和87.1%,其氨基酸序列的同源性均为90.0%。3种Prnp基因均有富含G-C的重复区,编码的PrP蛋白均由氨基端的信号肽、中间的成熟蛋白和羧基端的GPⅠ锚结合位点组成。基因型分析表明,秦川黄牛和甘肃土种绵羊可能存在感染海绵状脑病的风险。     

农村实用人才教育培训中存在的问题及对策

在大力推进社会主义新农村建设的今天,农村实用人才的教育培训问题显得尤为重要。本文通过对吉林省农村实用人才教育培训现状的分析,找出存在的一些问题,并提出了具体的对策建议。     

企业改制中资产评估的法律问题探析

我国目前资产评估法律环境有待完善,资产评估相关法律体系还没有建立起来。企业改制中资产评估主要遵守的是《国有资产评估管理办法》,不能满足资产评估业发展的需要。评估法律规范实质上是法律规范在评估中的具体应用,即评估法律规范是指由国家立法机构或国家行政机关依法制定的,是评估人员必须实施的行为规则,其最终目标是调整评估法律关系。     

Toxicological analysis of a fatal baclofen (Lioresal) ingestion

A fatality following ingestion of the drug baclofen (Lioresal) is described. Baclofen was identified in urine by gas chromatography/mass spectrometry. After derivatization with trinitrobenzene sulfonic acid, baclofen was quantitated in serum and urine by high-performance liquid chromatography. The concentration of baclofen was 17 mg/L in serum and 760 mg/L in urine collected approximately 12 h after the overdose. To our knowledge, this is only the second reported fatality involving a baclofen overdose. The previous case did not include quantitation of baclofen in any biological fluid.     

建国后17年文艺工作者的“组织化”及其评价

建国后，党对艺工作的思想、政治领导遂与组织领导结为一体，组织领导成了思想与政治领导的物质载体或曰制度保障。由之，思想与政治领导也就不自觉地转化成了行政领导关系。尽管这一领导方法在当时极有效率，但是，却在相当程度上违背了艺工作的“双百”方针。     

Plasma versus bone marrow desipramine: a comparative study

Correlation between plasma and bone marrow tricyclic antidepressants has not been studied before. Two groups of rabbits were given 10 and 20 mg of desipramine/kg body weight, respectively. Desipramine was administered to the animals once daily by mouth for 5 days. On the fifth day the animals were sacrificed and blood and bone marrow samples were collected and analyzed using a high performance liquid chromatographic (HPLC) method. Data showed that a correlation exists between bone marrow and blood desipramine. The bone marrow desipramine concentration increased as its blood levels increased. The average ratio of bone marrow to blood desipramine +/- S.D. (standard deviation) in both dosage groups was 37.2 +/- 4.46 with a range of 30.99-44.82. This investigation is promising and shows that bone marrow could be used as an alternative tissue in the absence of a suitable blood sample.