金黄色葡萄球菌疫苗候选分子StbA主要抗原表位区的表达及重组蛋白抗血清的制备

通过生物信息学软件分析金黄色葡萄球菌转铁蛋白结合蛋白(StbA)的主要抗原表位区,PCR扩增StbA主要抗原表位区的编码序列;将该序列插入表达载体pGEX-4T-1中,测序验证后转化大肠杆菌BL21(DE3)进行IPTG诱导表达。结果表明,重组菌能表达出分子质量约为54 000u的重组融合蛋白。表达产物经亲和层析纯化后可获得较高纯度的目的蛋白。Western-blot检测证实该重组蛋白能与乳腺炎患病奶牛恢复期血清发生阳性反应。ELISA结果显示,重组蛋白免疫新西兰大白兔能产生高效价的抗血清,提示StbA主要抗原表位区能刺激机体产生强大的免疫应答,StbA是一个有潜力的保护性抗原候选分子。     

在司法实践中检讨中国的商事留置权

针对海事法院经常受理的港口货物仓储合同、打捞合同、海上货物运输合同中经常出现的债权人错误行使留置权的案件,对我国现行有效商事留置权相关法律进行体系研究,并对临近其他成文法国家和地区的相关法律规定进行比较法研究,分析我国商事留置权法律制度构架之缺陷,并试图提出解决方案,以期丰满该制度,使商事留置权之行使清晰明确.     

A Noninvasive and Speculative Method of Visualizing Latent Fingerprint Deposits on Thermal Paper

Latent fingerprint deposits on thermal paper have been visualized noninvasively at visible wavelengths when illuminated with a UV‐A light source (peak 365 nm). A higher intensity UV source (250 W/m² at 0.38 m) gave superior fingerprint visibility when compared with a 60 W/m² (at 0.4 m) source. Removing the visible (blue) component of the light source emission did not adversely affect the visibility of the fingerprint. Sample fingerprints from 100 donors, when examined 24 h after deposition, produced identifiable fingerprints from nearly 34% of fingerprint deposits. A mechanism for the observed visibility is proposed based on low emission of visible wavelengths from areas of thermal paper coincident with the fingerprint deposit, when illuminated with UV. This is likely due to a weak color change in the thermal paper dye arising from protonated amino acid components of the sweat. This effect was not observed on nonthermal paper.     

Caustic Ingestion—A Forensic Overview

The ingestion of corrosive substances may produce severe burns to the upper aerodigestive tract and stomach, particularly if the pH is greater than 12 or less than two. There is a biphasic age grouping with adult cases most often involving self‐harm and pediatric cases accidental ingestion. Three cases are reported to demonstrate characteristic features following the ingestion of potassium hydroxide, glacial acetic acid and Lysol^®, respectively. All deaths were due to the effects of caustic burns to the upper aerodigestive tract, esophagus and stomach with perforation and/or hemorrhage. The extent of injuries in these cases depends on the nature, amount, and concentration of the agent and on the exposure time. A point to note at autopsy is that tissue damage may also occur from postmortem exposure. Typical injuries involve perioral, limb, and trunk burns, with extensive aerodigestive liquefactive/coagulative necrosis causing hemorrhage and perforation.     

Response Assessment of Thermal Papers from Four Continents to Fingerprint Development by Heat

Latent fingerprint deposits on thermal paper sourced from the U.S., China, the U.K., and Australia have been visualized by heating. U.S. and Chinese sourced paper produced two distinct types of fingerprint development. In one type (type 1), the paper dye colors where the deposit is present (as previously reported) and in the other type (type 2) the ‘inverse’ of this gives paper coloring only in areas not coincident with the deposit. Both development types gave identifiable fingerprints, the majority fading within 24 h of heating. Fingerprint development from U.K. and Australian sourced paper was exclusively type 1 and resistant to fading. Temperatures for fingerprint visualization were higher for U.S. paper (64–71°C) and Chinese paper (75–95°C) than for U.K. and Australian sourced paper (43–50°C). Particularly for Chinese sourced paper, these temperatures were within a few degrees of the normal paper color temperature. A mechanism for type 2 fingerprint development is proposed.     

论英语教学评估中反馈的作用

评估是英语教学的组成部分,反馈是评估不可或缺的重要环节,但在英语教学评估中没有得到应有的重视。应加强评估者对反馈重要性的认识,充分发挥反馈在评估和英语教学中的积极作用,促进学生语言技能的提高。     

It Takes a Coalition: The Community Impacts of Collaboration

Members of Congress frequently collaborate on policy initiatives, reaching out to colleagues in both parties to find common ground on solutions for the problems faced by their constituents. Using a novel dataset of over 30,000 “Dear Colleague” letters sent by members of the 111th Congress, I use the collaborative relationships that exist in the earliest stages of the policy process to measure the social influence of legislators. I demonstrate that districts represented by members of Congress who are better connected to their colleagues receive a greater share of federal grant money. I argue that this is because collaborative legislators are well‐positioned to be influential allies to strategic bureaucrats who want to maintain a broad base of support for their programs in Congress. Thus, devoting resources to building relationships with their colleagues is one way members of Congress can more effectively support the communities they represent.     

法国投资者适当性制度研究

法国投资者适当性制度主要体现在法国转化欧盟《金融工具市场指令》(MiFID)及其实施指令而经修正的法国金融市场管理局总条例(AMF General Regulation)中,并辅以《货币及金融法典》(Code Monétaire et Financier)。新形势下,法国投资者适当性制度面临新的考验和挑战。欧盟适时推出新的指令,旨在更好地提升投资者保护水平。