旧城改造与古都风貌保护—以东京为例

以东京为例,分析了其在"旧城改造"与"古都风貌保护"两方面是如何协调进行的,进而给大家以启示.     

论贩毒案件侦查中如何实现“控制下交付”——西部地区毒品犯罪对全国禁毒工作的影响研究之一

“控制下交付”已成为国际间和各国侦查毒品犯罪案件的重要方法。只有从毒品犯罪活动情报线索的发现入手 ,树立长期经营的思想 ,加强侦查工作的保密意识、精心设计侦查中的各个环节 ,才能保证成功实现“控制下的交付” ,稳、准、狠地打击毒品犯罪 ,完成禁贩缉毒任务。     

入世后开发区外企劳动关系值得注意的几个问题

入世后劳动关系问题成为国际劳工问题的一个突出问题 ,在劳动就业、劳资冲突以及劳资合作等方面呈现出了新的发展趋势 ,工会组织应认真研究和把握外企劳动关系的变化和发展趋势。     

语文兴趣教学初探

兴趣教学是有效完成语文课堂教学任务的重要手段,有其理论基础。兴趣教学有调节、激励、亲和等功能。语文教学中教师应努力探索和运用兴趣教学法,调动学生的学习积极性,让学生主动参与教学,发挥教学主体的作用,师生共同享受“快乐学习”。     

Validation of chest X-ray comparisons for unknown decedent identification

Comparing skeletal structures between antemortem and postmortem chest radiographs is widely used by forensic specialists from many disciplines to positively identify unknown decedents. However, validity assessments of this method have been fairly limited. This study had three objectives: 1) to quantify the reliability of ante- and postmortem chest radiograph comparison for decedent identification; 2) to identify useful radiologic features supporting decedent identification; and 3) to recognize sources of error in decedent identification related to use of comparative radiographs. A forensic pathologist, a forensic anthropologist, and two radiologists participated in the study. Our results showed that chest radiograph comparisons proved reliable, if basic decedent information was provided, and antemortem and postmortem radiographs were adequately positioned and exposed. A "morphological approach" using normal anatomical structures for comparison may provide the most efficient method for accurate identification.     

Environmental gas displacement: three accidental deaths in the workplace

The authors describe three accidental deaths resulting from occupational hazards involving environmental gas alterations. One involved the displacement of oxygen caused by leakage of liquid nitrogen during the installation of a magnetic resonance imaging system. Two involved elevated environmental carbon dioxide concentrations: dry ice sublimation in a walk-in refrigerator in a research laboratory, and activation of a carbon dioxide fire alarm-extinguisher system by a woman locked in a bank vault. The autopsy findings, scene investigations, and certifications of these deaths, as related to the mechanisms of death, are discussed.     

Recent developments in medicine and law

This article addresses important developments in the field of medicine and law during the period of September 2000 through August 2001. It does not discuss every reported case or piece of legislation but surveys some of the more significant developments in the medicine and law arena.     

重组纤维连接蛋白EDA-EDB融合蛋白的分子克隆

目的构建重组pET28a-EDA-EDB质粒,制备重组纤维连接蛋白EDA-EDB融合蛋白。方法采用基因重组技术,将EDA、EDB基因片段连接,再将该重组基因插入pET28a表达载体,表达重组融合EDA-EDB蛋白后,利用6×His/Ni-NTA纯化系统进行纯化,免疫印迹法检测。结果成功连接EDA、EDB基因片段,重组pET28a-EDA-EDB质粒;在大肠杆菌BL21(DE3)中高度表达重组蛋白,获得较纯的重组蛋白,免疫印迹法证实为FN的组分。结论EDA-EDB重组蛋白能采用pET系统在大肠杆菌中表达,并可通过6×His/Ni-NTA纯化系统进行纯化而获得免疫原。     

柱切换HPLC法分析尿中吗啡和O^6—单乙酰吗啡的研究

目的建立用柱切换HPLC技术分析尿中吗啡和06-单乙酰吗啡的方法.方法尿样用硼砂缓冲液(pH9.2)稀释后进入预处理柱(YWG-C18,33mm×5.0mm,10μm),用H2O洗去杂质,再用CH3OHH2O(6040)将被分析组分洗脱进入分析柱(Lichrospher(R)100CN,125mm×4.0mm,5μm),分析流动相为CH3OH磷酸盐缓冲液(pH6.86)=2278.紫外检测器波长为286nm.结果尿中吗啡和06--单乙酰吗啡的线性范围分别为50～1 600n/ml和100～1 600n/ml.吗啡和O6--单乙酰吗啡的精密度均小于4%.吗啡和O6-单乙酰吗啡的检测限均为40n/ml.结论用CSHPLC测定尿中吗啡和O6-单乙酰吗啡,方法准确、灵敏、快速、简便.