《民法典·婚姻家庭编》之我见

尽管 2 0 0 1年《婚姻法》(修正案 )取得了可喜的进步 ,但作为一个阶段性、过渡性的立法措施 ,其制度性的缺失以及内容的失之过简 ,难于操作 ,使其仍然有很大的修改空间。民法的法典化为婚姻法的第二步修订提供了极好的机会。完善婚姻家庭法就应当按照法律规范的科学性、前瞻性、实用性 ,全面、系统地对婚姻家庭关系的各项制度作出明确具体的规定 ,而非该制度的纲要性规范。     

犊牛前脂肪细胞的培养及其增殖与分化模型的建立

选择犊牛小肠网膜、附睾脂肪垫、腹部皮下脂肪组织材料 ,应用原代消化细胞培养法培养细胞。结果 ,由小肠网膜培养出了成分均一、增殖旺盛、分化率高的梭形细胞。经形态学动态变化观察、生长曲线测定、油红O脂肪染色及对胰岛素反应的测定 ,证明为功能活跃的前脂肪细胞 ,并在体外重现了其增殖、肥大的全过程。结果显示 ,小肠网膜脂肪组织存在着功能活跃的可调控的前脂肪细胞     

以人为本将新型生育文化融入企业文化

企业文化是以人为中心,以文化引导为手段,以激发职工的自觉行为为目的的一种企业经营管理思想,坚持以人为本,关注职工健康,不断满足职工日益增长的计生需求。     

公安机关“三基”工程建设的认识与思考

基层基础工作是整个公安工作的根基,是推动公安事业长远发展的基石。当前公安机关通过一年多的"三基"工程建设,取得了突出的成效,但由于公安基层工作还比较薄弱,离"三基"工程建设既定的目标还有较大的差距,要继续深入扎实的推进公安机关基层基础建设,必然要对制约公安机关基层基础建设的各种问题进行全面深入分析,并提出科学合理的对策。     

阿拉山口口岸走私珍贵动物及制品案件特点与对策

阿拉山口走私珍贵动物及制品案件多发生在国际列车旅检现场。具有内外勾结、走私数量大、规模大、形成蚂蚁搬 家式的完整走私链等特点。在侦办案件中要立足口岸,深入海关业务一线;情报先导,密织情报网;注重破大案,打团伙,抓 逃犯;加强国际合作,形成打击走私的强大合力。     

人体颅脑贯通枪创后心肌和肺的超微结构观察

本文报告6例人体颅脑贯通枪创后2小时所取心肌和肺的超微结构变化。6例心肌及肺的超微结构基本相似。心肌最明显的变化是:Z 带排列不规则,灶性肌原迁维溶解,线粒体肿胀、基质电子密度降低和嵴消失,间质水肿。肺组织的明显变化是肺泡隔和肺间质增宽伴电子密度降低,肺泡隔毛细血管内中性粒细胞聚集,肺间质或肺泡腔内中性粒细胞浸润。本文对主要病理变化的意义和发生原理进行了讨论,认为肺间质水肿与神经源性肺水肿有关,由于伤后经过时间长短不同或有无颅内高压形成,而出现间质性水肿或肺泡内水肿。心肌的变化与缺氧有关。     

丙酸钠和丙酮酸钠对体外培养牛肝细胞内丙酮酸羟化酶基因mRNA水平的影响

在成功构建牛肝细胞培养模型和PC基因DNA竞争模板的基础上,采用竞争RT-PCR方法检测了丙酸钠和丙酮酸钠对体外培养新生牛单层肝细胞PC基因mRNA丰度的影响。结果,随着丙酸钠浓度的升高,PC基因mRNA水平呈上升趋势,PC基因mRNA对丙酸钠的耐受范围较广;随着丙酮酸钠浓度的升高PC基因mRNA水平呈先上升后下降的趋势。结果表明,肝细胞内PC基因mRNA的表达水平受丙酮酸钠浓度的调控,在一定范围内丙酮酸钠对PC基因mRNA的转录具有促进作用,而浓度过高时则起抑制作用。     

Rural Southern China Takes on a New Image

THE year 2006 sees the launch of the Chinese government's campaign to "build a new socialist countryside." After more than 20 years of reform and opening-up, the focus is now on China's huge rural population. The     

Application of HLA-DRB1 genotyping by oligonucleotide micro-array technology in forensic medicine

The human leukocyte antigen (HLA) system is known to be the most complex polymorphic system in the human genome. Among all of the HLA loci, HLA-DRB1 has the second largest number of alleles. The purpose of this study is to develop an oligonucleotide micro-array based HLA-DRB1 typing system for use in forensic identification, anthropology, tissue transplantation, and other genetic research fields. The system was developed by analyzing the HLA-DRB1 (DRB1) genotypes in 1198 unrelated healthy Chinese Han individuals originating from various parts of China and residing in Shanghai, China. METHOD: Polymerase chain reaction (PCR) coupled with the oligonucleotide micro-array technology was used to detect and type HLA-DRB1 alleles of the sample individuals. The reliability, sensitivity, consistency and specificity were evaluated for use in forensic identification. Furthermore, a meta-analysis was carried out by comparing the allele frequencies of the HLA-DRB1 locus with those of other Chinese Han groups, Chinese minorities and other ethnic populations. RESULTS: All the DNA samples yielded a 273 bp amplification product, with no other amplification products in this length range. The minimum quantity of DNA detected by this method is 15 ng in a PCR reaction system of 25 microl. The population studied appeared to be not in Hardy-Weinberg equilibrium. Observed heterozygosity (Ho), expected heterozygosity (He), expected probability of exclusion (PE), polymorphic information content (PIC), and discrimination power (DP) of the HLA-DRB1 locus from the Shanghai Han ethnic group were evaluated to be 0.8022, 0.8870, 0.7741, 0.8771, 0.9750, respectively. A total of 25 HLA-DRB1 alleles were identified. HLA-DRB1*09XX, *04XX, *12XX and *15XX were the most frequent DRB1 alleles, which were observed in 58.76% of the sample. One hundred and sixteen genotypes were found. The five most frequent genotypes were: *04XX/*04XX (0.0626), *09XX/*09XX (0.0593), *04XX/*09XX (0.0551), *09XX/*15XX (0.0384) and *08XX/*12XX (0.0351). The meta-analysis showed that there were uniquely distributed features of DRB1 alleles among various ethnic populations and among the studied population groups from various regions with the same ethnic origin. CONCLUSIONS: An HLA-DRB1 genotyping system has been developed and established based on the oligonucleotide micro-array technology. The HLA-DRB1 typing of the Han population in Shanghai has revealed a relatively high heterogeneity. Information obtained in this study will be useful for medical and forensic applications as well as in anthropology research. Large-scale micro-array detection is highly accurate and reliable for DNA-based HLA-DRB1 genotyping. These results suggest that HLA-DRB1 DNA polymorphisms and the database of the Shanghai Han group have useful applications in processing forensic casework (as personal identification, paternity test), tracing population migration and genetic diagnosis.     

中国船员涉外人身伤亡救济模式研究

近年来多发的船员涉外人身伤亡案件体现了船员权益保护问题的紧迫性。船员涉外人身伤亡主要分为地点涉外和主体涉外两种,均可以船员国籍和船舶国籍为连接点适用中国法。目前我国调整船员劳动和劳务关系的条文散见于多部法律法规和司法解释,本文以最新船员法规、劳动法规、社会保险法规和2006海事劳工公约为依据,清理诸法头绪,结合海员外派制度,分析得出中国船员涉外人身伤亡主要有工伤保险、保赔保险、商业保险及损害赔偿诉讼四种救济模式,并详细研究各种模式的利弊。