以DFE为导向的环境教育课程设计研究——以《生态公园模型设计》课程为例

环境教育是提升环境行动能力的重要途径,对推进生态文明建设有重要意义。目前环境教育虽已有长足进步,但实际开展中仍存在内容散落、学科领域狭窄、实践性不足等问题。在分析中国环境教育现状与问题基础上,从环境教育综合性、实践性的特点以及引导学生主动参与解决环境问题的角度,建构了以DFE导向的初中环境教育课程模型,并以上海市初中为例进行《生态公园模型设计》课程案例设计与实践。学生在校内外教师共同组成的跨学科教学团队指导下,以设计者角色参与立足真实环境问题的DFE工程设计过程,采用校外实地调查和校内设计制作相结合的实践活动形式,帮助学生获得解决环境问题的能力,树立热爱环境的情怀,理解“人与自然是生命共同体”理念。     

抗禽脑脊髓炎病毒单克隆抗体的制备及鉴定

利用提纯的禽脑脊髓炎病毒(AEV)VR株CEF适应毒制备抗原,免疫BALB/c小鼠,应用杂交瘤技术以间接ELISA筛选获得1株分泌抗AEV(VR)单克隆抗体的杂交瘤细胞.对制备的细胞上清及腹水进行单克隆抗体特性鉴定,经染色体计数、免疫球蛋白类及亚类测定、特异性试验、稳定性试验、SDS-PAGE电泳,表明该杂交瘤细胞染色体数目介于90-110条,该单克隆抗体亚类为IgG2a,分子质量为142 ku,ELISA测定细胞上清效价为11×104,腹水效价为11×106,与其它病原无交叉反应,抗体分泌稳定.     

生物检材中吗啡类生物碱的LC-MS／MS分析

目的针对滥用药物分析鉴定实践中亟待解决的问题,开展LC-MS/MS分析生物检材中吗啡类生物碱的应用研究。方法满足不同的鉴定需要,分别建立血液、尿液、唾液和头发等生物检材的样品前处理方法,确定同时分析海洛因、单乙酰吗啡、吗啡、可待因、乙酰可待因、二氢可待因酮和氢吗啡酮等吗啡类生物碱的LC-MS/MS方法。将方法应用于实际案例。结果所建立的方法对吗啡类生物碱分离良好。尿液稀释法、尿液提取法和头发中吗啡的最低检测限(LOD)分别为10ng/mL、0.01ng/mL和0.01ng/mg。结论所建立的方法简便、快速、特异性强、灵敏度高。目标物中加入二氢可待因酮和氢吗啡酮扩大了方法的实用范围。     

侦破全国首例高考试卷被盗案的几点反思

今年四川省南部县发生了全国首例高考试卷被盗案。该案的成功告破,再次显示了 四川省公安机关的强大战斗力。同时也有一些经验教训值得反思。     

Analysis of penicillins in whole blood by reversed high-performance liquid chromatography

A reversed-HPLC method was established to determinate penicillins in whole blood. Thiamphenicol as internal standard was added to 0.5 ml blood. Proteins in blood were precipitated with acetonitrile, then the separate supernatant was directly injected onto the chromatography column. The range of five penicillins' linearities was 0.9918 to 0.9996, their range of relative recovery was 91.75% to 107.33%, their CV was 3.56% to 9.85%. Effects of different storage temperatures on the stability of analytes and interference of other antibiotics were also studied.     

The history of heroin abuse by assaying 6-monoacetylmorphine and morphine in human hair

The purpose of this study is to conclude the history of heroin abuse by assaying 6-monoacetylmorphine (MAM) and morphine (MOR) in human hair. The hair of heroin abuse was labeled and segmented, then washed and cut into fragments. After hydrolyses and extraction, 6-MAM and MOR in human hair were determined by GC/MS-SIM with selected ion monitoring. Results of the segmented hair were analyzed. It provided useful information about the history of heroin abuse (hair growth rate 1-1.5 cm/mon).     

知情权在我国的确认与实现刍议--写在中国记者节确立之日

知情权是一项为当今国际社会普遍认同的基本人权和民主权利。我国从宪法和法律上明确承认和肯定知情权是健全民主政治、清除腐败和保护与扩大公民权利的需要。知情权的充分实现需以政府和新闻界的足够尊重、公民的表达自由以及知的文化价值观念和追求作为必要条件。     

试析中国处理中印边境武装冲突的斗争艺术

１９６２年的中印边境自卫反击战，作为军事、政治、外交斗争的综合较量，体现了中国共产党人非凡的政治胆识和高超的斗争艺术。研究６０年代中国政府为捍卫祖国领土完整、打击地区扩张主义、解决边界冲突与矛盾所采取的原则立场，无疑有助于中印边界问题的最终解决。 中印边界全长２千公里，分东、中、西段，历史上从来没有正式划定过，是中印关系中存在的历史遗留问题。１９世纪中叶以前，双方根据历史行政管辖所及，形成了传统习惯线，两国地图均按传统习惯线标画边界。然而，在英国统治了印度后，中印边界被打上了殖民烙印。在东段，英…     

南疆重镇喀什

<正>Your journey starts here.In fact Kashgar may be both point of departure and last stop in beginning to understand Xinjiang.Like small flakes of an ancient mirror embedded in a pillow,today’s Kashgar Prefecture in far western China reflects past times when it was a central station for bartering,the precious goods trade,cultural and religious infusion,and wealth accumulation.Abutting the Taklamakan Desert,Kashgar through the     

Breaking point? An analysis of fatal stab wounds to the torso in Ireland between 2011 and 2018, examining the extent of rib fractures

This study examines the pattern of rib injuries occurring in cases of fatal torso stab wounds in Ireland between 2011 and 2018. It has been suggested by previous studies that rib fractures are not commonly sustained in stab wounds to the torso. We wanted to ascertain whether this was the case, as our data suggested that rib fractures were frequent, and where a rib is fractured there is a higher chance of organ injury and death, making this an important area of study. One hundred and forty seven cases of fatal stab wounds from an eight-year period were retrospectively reviewed. Fatal stab wounds to other body areas, were excluded; leaving a total of 104 cases with stab wounds to the torso. We found that 69.2% of cases had rib injuries, a figure significantly higher than previously reported. Our data suggests that stab wounds to the torso often fracture ribs, putting the underlying organs at increased risk of injury and perhaps contributing to fatality. The amount of force needed to cause a rib fracture can be difficult to quantify and indeed from the high percentage of rib fractures sustained in our data it appears that the ribs may be fractured regardless of the amount of force used; this is borne out by the finding that self-inflicted injuries also caused rib fractures. Our study shows that other factors, such as anatomical positioning and wound depth may have a greater bearing than force in terms of whether a rib fracture is sustained.