蛋白酶K-Chelex100法提取肋软骨DNA技术的优化

目的优化蛋白酶K的用量和消化时间,用于提取肋软骨DNA。方法 30份肋软骨样本各取大小为0.2cm×0.3cm×0.4cm的软骨块5份,分别编为A～E组。采用不同蛋白酶K用量和消化时间的Chelex100方法,提取5组各30份软骨块DNA,进行DNA定量、采用Sinofiler试剂盒扩增后进行电泳检测,采用非参数检验法比较检验成功率。结果 A～E组样本中采用加入2μL蛋白酶K并消化30min的D组方法成功率最高(96.7%),组间差异具有统计学意义。结论采用蛋白酶K-Chelex100方法,选择加入2μL蛋白酶K,消化30min可有效提高肋软骨DNA分型检验成功率。     

诱惑性侦查比较研究

诱惑性侦查违背了罪刑法定原则,违反了刑事诉讼的被动性和程序正义理念,并且通过诱惑性侦查所取得的证据是非法证据。因而,原则上应当禁止诱惑性侦查。但是,为了打击犯罪的需要,在例外情况下,可以允许有条件地使用诱惑性侦查手段。     

论我国司法官选任制度的完善

司法是社会正义的最后防线,司法正义的实现必须拥有一批高素质的司法官队伍。在构建我国司法官选任制度时,应当借鉴法治发达国家的有益经验和做法,提高司法官任职的最低学历条件,明确司法官的法律职业经验要求,完善国家司法考试制度,强调司法官的品行操守,建立司法官任职培训制度,完善司法官的选任机制和程序。     

泰国南疆地区暴力袭击活动的特点及成因分析

本文从泰国南疆地区暴力袭击活动的特点着手,对暴力袭击的手法、目标、时间、地点等因素进行归类分析,并与国际恐怖主义袭击的特点进行比较,从多角度探讨该地区暴力袭击持续多年并无法平息的原因。本文认为,泰国南疆地区持续动乱的原因非常复杂,不是单一因素作用的结果,它缘于民族分离主义运动,但却又受制于泰国国内政治、经济等因素。只要泰国的国内政治形态没有变化,国外势力没有进一步介入,南疆动乱仍将持续,但却不会产生从量变到质的变化。     

Spatial and Temporal Patterns of Terrorist Attacks by ETA 1970 to 2007

Rational choice perspectives maintain that seemingly irrational behavior on the part of terrorist organizations may nevertheless reflect strategic planning. In this paper we examine spatial and temporal patterns of terrorist attacks by the Spanish group ETA between 1970 and 2007. Our analysis is guided by a public announcement by ETA in 1978 that the group would shift from emphasizing attacks in the Basque territory to instead launch attacks more widely in the hopes of exhausting the Spanish government and forcing it to abandon the Basque territory. This announcement suggests that prior to the end of 1978 ETA attacks were based mostly on controlling territory in the Basque region that they hoped to rule; and after 1978 the organization decided to instead undertake a prolonged war of attrition. Accordingly, we argue that before the end of 1978 ETA was mostly perpetrating control attacks (attacking only within the Basque territories) and that the diffusion of attacks between provinces was mostly contagious (spreading contiguously). After the 1978 proclamation, we argue that the attack strategy shifted toward attrition (attacking in areas outside of the Basque territories) and that the attacks were more likely to diffuse hierarchically (spreading to more distant locations). As predicted, we find that after ETA moved toward a more attrition based attack strategy, subsequent attacks were significantly more likely to occur outside the Basque region and to target non-adjacent regions (consistent with hierarchical diffusion). We also find that hierarchical diffusion was more common when a longer time elapsed between attacks (a likely consequence of the fact that more distant attacks require more resources and planning) and that attacks against Madrid were unlikely to be followed immediately by more attacks on Madrid or surrounding provinces. After ETA announced a shift in policy, they maintained a highly dispersed attack strategy even during their period of decline. Using information about where and when prior attacks occurred could provide useful information for policy makers countering groups like ETA.     

广告语:短句中的法律复杂性

广告语涉及众多复杂的法律问题。广告语具有可版权性的特点,表达量并非独创性的决定性因素,相反,文字的简洁性恰好是独创性的表现;高度提炼后的表达,不能简单地归为思想的范畴。广告语及其组成片段具有识别功能,一旦获得第二含义,即具有保护的必要性。对广告语的侵犯关涉竞争秩序,运用一般条款进行商业伦理的判断,有利于保护广告语。     

技术措施保护的隐私权限制

版权人通过技术措施收集个人身份信息,跟踪监测作品使用行为,甚至进入电脑系统进行私力技术救济,给消费者的信息隐私带来严重的威胁。为此,我国应规定技术措施不得具有隐私侵害性,限制技术措施的信息收集范围和收集方式,赋予消费者规避侵害隐私的技术措施的权利,规范版权人对消费者信息的处理利用规则。     

论法院对基本权利的保护

为公民基本权利提供保护,是现代国家合法性的重要来源。普通法院在基本权利保护中发挥着重要作用。德国虽然设立了宪法法院,但保护公民基本权利的职责仍主要由其他法院承担。在没有设立宪法法院的国家,通过普通法院更是为基本权利提供司法保护的唯一途径。我国法院应当通过三种方式为基本权利提供司法保护:如果法律有保护基本权利的具体规定,法院应当直接适用法律;如果法律对基本权利的保护只作了抽象规定,法院应当对有关抽象立法进行合宪解释之后予以适用;如果法律没有作出保护基本权利的任何规定,法院可以直接适用宪法的基本权利条款。     

“解严”后政党角力下台湾新闻自由的进步与迷思

台湾新闻自由实践在其政党角力中扮演着工具角色。换言之,新闻自由从来就不是政党角力的目的,只是权力斗争的工具。多党纷争时期,在野党高举“新闻自由”旗帜,与国民党争夺媒体资源。政党轮替后,对峙的国民两党都是表面附和“新闻自由”,实际上却采用隐性、间接的方式操控媒体。政党竞争带来了广电媒体的解禁、新闻自由法律法规的修正、新闻自由观念的成熟等正面效应。但政党对媒体的变相操控却让新闻自由异化,“只问政党,不问事实”的蓝绿媒体难以承担“第四权”责任。     

GC Analysis of Black Gel Pen Ink Stored under Different Conditions

In many criminal and civil cases in China, the most commonly questioned documents are those written with gel pen ink. An important task for forensic document examiners is to identify whether two or more ink entries in one or more documents were written with the same ink type. The identification of the age of gel ink entries made poses an important and difficult problem for forensic document examiners. In this paper, the volatile components of gel ink were determined and the gel ink was classified by gas chromatography with a flame ionization detector. Calibration curves were created to express the relationship between the content of volatile gel ink components and the age of gel ink entries stored under natural and UV‐induced aging conditions. The correspondence between the natural and UV‐induced aging conditions was also established. The experimental results showed that GC was useful in the analysis of black gel ink and applicable for determining the relative age of gel ink entries under certain conditions.