多重PCR检测FFv三个基因座在景颇族人群中的遗传多态性

短串联重复序列（STR）是由几个碱基对作为核心单位串联重复形成的一类DNA序列,作者将3个STR基因座在同一反应体系中进行互不干扰的复合扩增,采用高分辨率的聚丙烯酰胺凝胶电泳分离、银染法显影技术,对云南省景颇族的F13A01,FESFPS和vWA等3个基因座等位基因的基因频率进行了调查,获得了满意的结果,显示了广阔的应用前景。F13A01基因座观察到8个等位基因、13个基因型;FESFPS基因应观察到7个等位基因、18个基因型;vWA基因应观察到7个等位基因、21个基因型。     

HLA血型亲权鉴定系统分析软件的开发及应用

应用FoxPro2．5系统开发了HLA亲权鉴定软件,程序运行中有汉字提示,使用方便。对63例HLA亲权鉴定案例进行复核计算,所得结果与人工计算结果一致,证明程序计算结果可靠,可在实际检案中应用。     

甲醛固定组织中DNA提取与扩增技术的初步研究

目的：探索从甲醛固定组织中提取并扩增DNA的方法。方法：采用高pH缓冲液,结合有机溶剂法抽提DNA,选择扩增片段较短的DQa位点作为检测位点。结果：甲醛固定组织中的DNA降解程度随浸泡时间的延长而加重,但浸泡1、5、10个月的3份心脏组织均获得了240bp的扩增产物。     

Analysis of 226 cases of psychosis involved in lawsuit

226 cases of psychosis involved in lawsuit were investigated. The problem of forensic identification was discussed. Some suggestions were given to raise the quality of the forensic identification in psychosis.     

A survey of the social and psychological factors of the heroin dependence

The survey studied 100 dependence about their age, marriage, occupation, the way of heroin taking, history of heroin taking, and the reason of the first heroin taking. The data showed that most dependence were young or middle aged men, or people unable to graduate from junior middle school, self-employed laborers and unemployed. Dependence of cadres and people with better education were increasing. The heroin injection is becoming popular.     

Book reviews

He Bochuan, China on the Edge: The Crisis of Ecology and Development, Berkeley: Pacific View Press, 1991.

Hu Ping, Zai lixiangyu xianshi zhijian (Between Ideal and Reality). Hong Kong: Tianyuan shuwu, 1990.

Hu Ping, Gei wo yige zhidian (Give me a Fulcrum). Taiwan: Lianjing chuban Gongshi, 1988.

Shi Bo, Waimenggu duli neimu (The inside story of Outer Mongolia's independence) (Beijing: Renmin Zhonguo chubanshe, 1993), 479pp, illustrations, maps.

Michael David Kwan, Broken portraits: Personal encounters with Chinese Students, San Francisco: China Books & Periodicals, 1990.

Liang An Guanxi yu Zhongguo Qiantu: Xueshu Yantaohui Lunwenchi (The Taiwan‐Mainland Relationship and the Future of China: Proceedings of a Scholarly Conference), Taipei, Taiwan: Minchu Jijinhui (Democracy foundation), 1992. 390 pages.     

HLA-DRB1位点的PCR-SSP分型在亲子鉴定中的应用研究

应用PCR－SSP（PCRamplificationwithsequencespecificprimer）方法将HLAⅡ类DRB1位点基因分型应用于亲权鉴定。对42例亲子鉴定案例进行分析研究的结果表明,本方法简单、快速、结果可靠,且具有较高的非父排除概率（66．3％）,不仅适用于法医学亲手鉴定和个人识别,亦可应用于移植配型、HLA相关疾病及人类遗传学研究。     

Study of the high polymorphism STR locus-FGA in Chinese population

Population genetic study of the complicated STR locus-FGA was performed by the method of PCR in Han population samples. A total of 21 different alleles including 7 interalleles and 63 different genotypes were observed in 349 unrelated individuals. The observed genotype discrimination showed no significant deviation from Hardy-Weinberg equilibrium and 51 family studies showed no mutation. According to the results obtained in this study(DP = 0.9612, PE = 0.7007, PIC = 0.8333), this system can be used as an useful means in forensic identification of both criminal and paternity case.     

Polymorphism analysis of D19S253 locus in Guangzhou area population

Frequency data for STR system D19S253 were obtained from 105 unrelated individuals of Guangzhong population. PCR products were detected by horizontal native polyacrylamide gel electrophoresis and a total of 9 alleles were identified by side-by-side comparison with a sequenced allelic ladder prepared by ourselves. The observed genotype distribution conformed with Hardy-Weinberg equilibrium. The high information content found in this system(heterozygosity rate was 0.8353, the mean exclusion chance was 0.7499, the discrimination power was 0.9211, the polymorphism information content was 0.8203) indicated it is a useful means in forensic routine casework both in criminal and paternity cases.