全文获取类型
收费全文 | 2299篇 |
免费 | 76篇 |
国内免费 | 2篇 |
专业分类
各国政治 | 79篇 |
工人农民 | 71篇 |
世界政治 | 87篇 |
外交国际关系 | 108篇 |
法律 | 1212篇 |
中国共产党 | 98篇 |
中国政治 | 257篇 |
政治理论 | 160篇 |
综合类 | 305篇 |
出版年
2023年 | 5篇 |
2022年 | 14篇 |
2021年 | 31篇 |
2020年 | 54篇 |
2019年 | 31篇 |
2018年 | 36篇 |
2017年 | 39篇 |
2016年 | 42篇 |
2015年 | 34篇 |
2014年 | 54篇 |
2013年 | 127篇 |
2012年 | 78篇 |
2011年 | 125篇 |
2010年 | 135篇 |
2009年 | 113篇 |
2008年 | 147篇 |
2007年 | 126篇 |
2006年 | 143篇 |
2005年 | 130篇 |
2004年 | 81篇 |
2003年 | 77篇 |
2002年 | 73篇 |
2001年 | 136篇 |
2000年 | 86篇 |
1999年 | 85篇 |
1998年 | 42篇 |
1997年 | 11篇 |
1996年 | 9篇 |
1995年 | 12篇 |
1994年 | 9篇 |
1993年 | 10篇 |
1992年 | 22篇 |
1991年 | 19篇 |
1990年 | 40篇 |
1989年 | 27篇 |
1988年 | 30篇 |
1987年 | 24篇 |
1986年 | 15篇 |
1985年 | 14篇 |
1984年 | 11篇 |
1983年 | 12篇 |
1982年 | 11篇 |
1981年 | 10篇 |
1980年 | 7篇 |
1979年 | 12篇 |
1978年 | 7篇 |
1973年 | 2篇 |
1971年 | 4篇 |
1970年 | 3篇 |
1968年 | 2篇 |
排序方式: 共有2377条查询结果,搜索用时 15 毫秒
991.
Simultaneous detection of multiple STR loci on sex chromosomes for forensic testing of sex and identity. 总被引:2,自引:0,他引:2
Z Tun K Honda M Nakatome M Nakamura S Shimada Y Ogura H Kuroki M Yamazaki M Terada R Matoba 《Journal of forensic sciences》1999,44(4):772-777
The forensic usefulness of X and Y chromosomal STR loci has recently been demonstrated. One quadruplex-PCR, using 2 X- and 2 Y-STRs (STRX1/HPRTB and DYS390/ DYS393), and 2 duplex-PCRs, each using an X- and a Y-STR (ARA/DYS390 and ARA/DYS393), and detection of PCR products by using an automated DNA sequencer are reported herein. This approach allows us to determine not only the sex of the donor of a sample, but also the X- and/or Y-STR genotypes of the sample. A male biological specimen yields 4 amplified products in quadruplex-PCR and 2 amplified fragments in duplex-PCRs, whereas a female biological specimen yields only 2 amplified fragments of X-STR in quadruplex-PCR and one fragment, also of X-STR, in duplex-PCRs. Our study thus provides useful information for many activities in forensic practice, such as identity testing, paternity testing, especially of deficiency cases, compilation of population data, and sex determination of a biological sample from a single PCR. 相似文献
992.
Y Bunai A Nagai I Nakamura S Kanno S Yamada I Ohya 《The American journal of forensic medicine and pathology》1999,20(3):256-260
We describe an unusual case of fatal gas embolism, in which a man died by connecting an extension tube supplying oxygen to an indwelling catheter that was inserted into the left cephalic vein. 相似文献
993.
After amplification by polymerase chain reaction (PCR), the nucleotide sequences of a 452-bp section of the D-loop region of human mitochondrial DNA (mtDNA) were determined in 40 teeth extracted from patients living in Kanagawa prefecture, Japan. Dental DNA was extracted separately from the dental pulp and dentin (i.e., the attached pulp cells from the most superficial layer of the pulp cavity wall) of the same tooth. Comparison of the nucleotide sequences of the 452-bp region of the D-loop demonstrated that nucleotide substitutions and insertion/deletion events were identical in material from both sources. Thus, dentin produces equivalent results when the dental pulp of a tooth is unsuitable for mtDNA analysis. To establish the reliability of the screening procedure for the sequence analysis, we identified restriction sites for the enzymes KpnI and MnlI in the 452-bp region of the D-loop. Thirteen of 14 patterns of four polymorphisms analyzed using the mtDNA from the 40 tooth samples were identifiable by an initial screening procedure involving restriction fragment length polymorphism (RFLP) analysis. Combined use of sequence analysis and RFLP analysis proved extremely efficient in analyzing mtDNA polymorphisms, allowing identification of individuals. 相似文献
994.
995.
GC/MS研究海洛因代谢物在吸毒者体内的分布 总被引:5,自引:2,他引:3
应用GC/MS-SIM测定了海洛因代谢物吗啡在两例海洛因延缓死亡者的体内分布,并分析了收集到的7例案子的毛发(头发、腋毛和阴毛)中6-单乙酰吗啡和吗啡含量。生物检材和毛发经酸水解、提取、醋酸酐或双(三甲基硅基)三氟乙酰胺(BSTFA)衍生化,然后进行GC/MS-SIM分析。结果表明尿、胆汁和肝脏是判定死者是否曾使用海洛因的最佳检材;毛发分析与生物检材相比有其独特的优点,可提供数月甚至数年的用药情况。为公安司法机关打击吸毒、惩治犯罪提供更可靠、有效的证据。 相似文献
996.
意外摔跌致颅脑损伤21例尸体检验分析 总被引:2,自引:0,他引:2
通过对21例意外摔跌伤所致颅脑损伤的法医学检验资料进行统计分析,探讨意外摔跌伤的损伤特点、发生原因,以及与高处跌倒、交通事故及打斗中摔跌形成的损伤特点进行比较。结果表明意外摔跌饬有其独特的特点。 相似文献
997.
A reversed-HPLC method was established to determinate penicillins in whole blood. Thiamphenicol as internal standard was added to 0.5 ml blood. Proteins in blood were precipitated with acetonitrile, then the separate supernatant was directly injected onto the chromatography column. The range of five penicillins' linearities was 0.9918 to 0.9996, their range of relative recovery was 91.75% to 107.33%, their CV was 3.56% to 9.85%. Effects of different storage temperatures on the stability of analytes and interference of other antibiotics were also studied. 相似文献
998.
44 cases of adrenal hemorrhage (AH) were analyzed. The forensic autopsy showed that AH occurred mainly in male aged from 20 to 40 years old. The traumatic AH was more often seen in young men and the pathologic AH was mostly seen in elderly persons. AH were more likely found in bilateral adrenal corticomedullary junction. The pathological grade of AH was not related to the interval time of survival. The pathologic mechanism of AH was discussed as well. 相似文献
999.
The cortex and brainstem changes in death from head injury were observed. There were hemorrhage, edema and neuron necrosis in brain injury group, and also in disputed group of brainstem injury. 相似文献
1000.
The allele and genotype frequencies of 6 tetranucleotide STR loci were investigated in a sample of 132 unrelated individuals from Chinese Han population. The PCR products were analyzed on 6% denaturing PAGE and detected using fluorescently labeled primers in an automated 377 sequencer(PE). All loci meet Hardy-Weinberg equilibrium. There was no random association of alleles among the 6 loci. The allele frequencies were compared with other population databases. Except locus vWA31A, the observed heterozygosity at other 5 loci was significantly lower than that reported in Caucasian and Black population studies. The calculated DP = 0.99999, PE = 0.9708, pM = 1.059 x 10(-5). The allelic frequency data can be used in forensic identification and paternity testing. 相似文献