立法保护合法竞争加速市场经济体制的建立

<正> 一、立法保护竞争的必要性、迫切性、可能性中国共产党的第十四次代表大会,确定了中国今后将建立起社会主义市场经济体制,这是一个宏伟的战略目标,符合亿万人民的心愿,是客观历史发展的必然。从世界范围看,市场是能把各种产品、生产要素的买者和卖者连结起来的交换机制,是实现社会资源配置的基本手段。市场经济制度就是一种能保证这种交换机制、资源配置手段得以正常、顺利运作的经济制度。这种经济制度最基本的特征之一就是开展市场竞争。竞争机制是一切形式的商品经济最重要的运行机制之一。它要求市场参与者在进入市场和进行商     

活体鉴定400例放射学特点分析──兼论法医临床学与放射学的关系

为探讨法医临床学鉴定与放射学的关系，本文对４００例活体鉴定就放射学检查的有关问题进行回顾性研究。结果表明：２６６例进行了放射学检查（占６６．５％），其中Ｘ线１７６例（６６．２％），ＣＴ４３例（１６．１％），两者均有的４７例（１７．７％）。放射学检查部位中，Ｘ线以四肢为多，ＣＴ以颅脑为多。误诊率为９．０２％，放射学检查与致伤原因之间有一定关系，轻微伤中不必要的ＣＴ检查多见。并就放射学误诊的法律后果及不必要的放射学检查问题进行了讨论。本文从法医临床学的角度对放射学进行了评价，指出在活体鉴定中可以应用放射学知识协助证明损伤、推断损伤形成机制、个人识别及研究损伤时间。因此法医学鉴定人必须有放射学理论和技术方面的知识。作者还试图提出法医临床放射学。     

灌服毒鼠强诱导大鼠细胞DNA的损伤研究

目的研究毒鼠强体内染毒后,毒鼠强对大鼠脑细胞、心肌细胞、淋巴细胞DNA的损伤作用。方法选择健康Sprague-Dawley大白鼠20只,分成5组,每组4只,采用灌胃方法使大鼠毒鼠强体内染毒,按0.2,0.1,0.05,0.01mg·kg-1制作大鼠毒鼠强中毒模型,并以灌服生理盐水的健康大鼠为对照,分离实验大鼠的淋巴细胞、心肌细胞和脑细胞,用彗星电泳的方法测定不同浓度毒鼠强中毒后的细胞DNA损伤。结果0.2,0.1,0.05,0.01mg·kg-1剂量组的毒鼠强均可引起大鼠的淋巴细胞、心肌细胞和脑细胞DNA损伤,均与对照组差异有显著性(P<0.01)。结论毒鼠强诱导体内细胞DNA损伤可能是毒鼠强毒性作用机制之一。     

行为加重犯研究

行为加重犯是一个崭新的概念.文章通过对加重犯基本构成及加重因素的分析研究,提出所有的加重因素都可以被称为"情节".情节加重犯的概念要分广义和狭义两种.在广义的情节加重犯中应当剥离出行为加重犯,成为独立的加重犯形式,并与结果加重犯、数额加重犯、狭义的情节加重犯一起形成加重犯完整的理论体系.     

Genetic data of 9 STR loci from Henan Province (central China)

Allele frequencies of the nine short tandem repeats (STR) loci D8S1179, D21S11, D18S51, D5S818, D3S1358, D7S820, vWA, FGA (AmpFlSTR Profiler Plus) were determined in a population sample of unrelated individuals living in central China.     

Analysis of 15 STR loci in Chinese population from Sichuan in West China

Allele frequencies for 15 STR loci found in PowerPlex 16 System kit were determined in a sample of 200 unrelated individuals living in Sichuan area in west China. The values of observed heterozygosity (Ho), power of discrimination (PD), probability of paternity exclusion (PE) and polymorphism information content (PIC) were calculated. All loci were in accordance with Hardy-Weinberg equilibrium (p<0.05). The obtained frequency distributions were compared with other previously reported population data.     

Chelex-100法提取滤纸血痕DNA影响因素的比较

目的改进滤纸血痕DNA提取方法,建立更简便、廉价,适合当前DNA建库需要的提取方法。方法将752份滤纸血痕分成四组,分别按照四种不同的Chelex-100法进行DNA提取并进行比较研究;63份新鲜血痕分别按照两种方法提取并进行对比研究。结果对于陈旧滤纸血痕,四种提取方法的检测成功率无显著差异(P>0.05);对于新鲜血痕,两种提取方法的检测成功率有显著差异(P<0.05)。结论对于建库陈旧滤纸血痕样本的DNA提取可采用不加纯水处理,直接加入Chelex-100的方法进行。     

液相色谱-串联质谱法检测血液中的美西律

目的建立测定血液中美西律(mexiletine)的液相色谱-串联质谱联用法(LC-MS/MS)。方法采用简便的乙腈蛋白沉淀法对血液进行预处理,应用Allure PFP Propyl液相柱分离,用电喷雾正离子模式离子化,多反应监测模式对美西律进行分析。结果美西律与内标纳洛酮分离良好,在0.02～10.00μg/mL内线性关系良好,相关系数为0.9999,回归方程为y=0.0283x-0.0151,日内与日间精密度的RSD均小于15%,最低检测限为0.01μg/mL。结论建立的LC-MS/MS方法简单、灵敏、可靠,可同时适用于美西律临床药物监测和法医毒物分析的需要。     

不同死亡方式大鼠心肌组织中ATP、ADP和AMP含量比较

目的比较不同死亡方式对大鼠心肌组织能量物质变化的影响,为判断不同死亡方式及死亡时间提供依据。方法180只大鼠随机分为三组,以不同方式(失血、窒息、断颈)处死,高效液相色谱法检测死后0、0.5、1、1.5、2、3、4、6、8、12、18和24h大鼠心肌组织中的ATP、ADP、AMP含量。结果三组不同死亡方式大鼠心肌组织的ATP、ADP、AMP的含量在大部分时间点做组间比较有明显差异(P<0.05);每组不同死亡方式的大鼠心肌组织中ATP、AMP的含量随时间的变化有明显的差异(P<0.01),而各组中的ADP在死亡一定时间后无明显差异。结论不同死亡方式的大鼠心肌组织中能量物质死后变化可以用作对死亡方式及某种方式不同死亡时间做出判断的依据。     

Induction of hypoxia-inducible factor-1alpha in two kinds of rats asphyxiation death models

OBJECTIVE: To investigate the expression of hypoxia-inducible factor 1-alpha (HIF1-alpha) in the heart, lung, liver and kidney in rats died of two typical models of asphyxia. METHODS: Two asphyxia models were made and tissue samples of the dead rats were collected from different groups at various postmortem duration. The expression and the changes of HIF1-alpha in various tissues were examined by immunohistochemistry and image analysis techniques. Results Significant expression of HIF1-alpha was observed in the myocardial fibers, kidney cells, liver cells and lung cells in both asphyxia models, but not in the control group. The expression of HIF1-alpha in various tissues in the rat died of nitrogen gas breathing was found in the nuclei at 0 hour and the expression level decreased gradually thereafter. The HIF1-alpha expression level and duration in various tissues of the rat died of hanging were higher and longer than that of the former group, with a peak of the expression level observed 6 hours after death, and then started to decline in all tissues except the heart where the expression still showed an increase 24 hours after death. The control groups showed a steady expression in the cytoplasm but not in the nuclei. CONCLUSION: HIF1-alpha appears to be a valuable biomarker in the diagnosis of asphyxia within 24 hours after death.