Correlation of methamphetamine results and concentrations between head, axillary, and pubic hair

This study was designed to compare the qualitative results and concentrations of methamphetamine (MA) and its metabolite amphetamine (AP) in head hair and hair collected from different parts of the body (axillae and pubis). Hair from subjects (N = 14) suspected MA users was simultaneously collected. Hair preparation involved washing step, fine cutting, overnight extraction, derivatization by the trifluoroacetic anhydride, and gas chromatography/mass spectrometry (GC/MS) using selective ion monitoring. In this study, we found a good correlation of the qualitative results for MA between head hair and hair on other parts of the body, but there were some differences in concentrations of MA and AP. Namely, the concentrations of MA and AP were higher in axillary and pubic hair than in head hair.     

Population data for the AmpFl STR Identifiler PCR Amplification Kit in China Han in Jilin Province, China

Allele frequencies for the fifteen short tandem repeats (STR) loci D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, D19S433, THO1, D16S539, CSF1PO, TPOX, D2S1338 (AmpFl STR Identifiler PCR Amplification Kit, Applied Biosystems) were determined in a population sample of unrelated China Han in Jilin Province, China. Statistical analyses were performed using Powerstats version 1.2 and GEP-ISFGWG. The results showed that all the loci met Hardy-Weinberg expectation.     

The prevalence of MDMA/MDA in both hair and urine in drug users

The prevalence and age distribution of 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyamphetamine (MDA) in hair samples by gas chromatography/mass spectrometry (GC/MS) were studied. The recoveries obtained from hair were 97% and 99% for MDMA and MDA, respectively. The inter- and intra-assay precision and accuracy were determined. Out of 791 hair samples, 44 (5.6 %) contained MDMA and/or MDA. Out of these 44 subjects, urinalyses from 35 were negative for both MDMA and MDA, while only 9 were positive. We also evaluated concentrations of MDMA and MDA, and the metabolite-to-parent drug ratios. This study showed that the abuse of MDMA or MDA was found principally among young adults and male abusers. We found the epidemiology of ecstasy users in Korea between March 2002 and April 2003.     

Study on the application of parent-of-origin specific DNA methylation markers to forensic genetics

In paternity test, especially in motherless cases, the allele inherited from father (obligatory gene, OG) often cannot be determined. The paternity exclusion probability (PE) of a genetic marker is reduced considerably. Therefore, it is necessary to develop a new technique, by which the parental origin of alleles can be determined without genealogical analysis. In this paper, we explored the possibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles, choosing the imprinted single nucleotide polymorphism (SNP) locus rs220028 (A/G) as a model system. We typed the SNP by mutagenically separated PCR (MS-PCR). The frequencies of alleles were A = 0.5085, G = 0.4915; the unbiased heterozygosity was 0.5020. In order to discriminate between the maternal allele and paternal allele, post-digestion MS-PCR, a novel PCR based methylation analysis and SNP typing technique was developed and performed on 18 heterozygous children, and the methylated maternal allele was detected specifically. As a pilot study on the use of epigenetic markers in forensic genetics, our results demonstrated the feasibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles.     

论城市牛皮癣的综合治理

"城市牛皮癣"不仅影响了城市环境的美观整洁,严重污染了市容市貌,也使一些制贩假证件、假文凭、私刻假印章、兜售假发票等不法活动日益猖獗,扰乱了正常的社会秩序,侵害了国家、集体的公共利益和有关单位的合法权益。要彻底根除"城市牛皮癣",还都市靓丽形象,必须标本兼治,综合运用行政、法律、舆论、教育等手段,动员城管、公安、电信、市民等一齐动手,举全社会之力,打一场围剿"城市牛皮癣"的"人民战争"。     

甲基安非他命（冰毒）诱导基因表达变化研究进展

甲基安非他命进入机体后,其最根本的变化是引起了成瘾者许多基因转录和表达改变。这些基因包括与神经元损害有关的基因、与日节律有关的基因、与行为异常有关的基因及其它一些无法分类的基因。它们基因转录和表达水平增高或者降低引起了甲基安非他命成瘾者各种临床表现。研究这些基因表达可以为法医学鉴定提供依据。     

试论俄罗斯不批准《京都议定书》的原因和法律后果——以《气候变化框架公约》第九次缔约方会议为例

在<气候变化框架公约>第九次缔约方会议上,由于俄罗斯仍然拒绝批准<京都议定书>,致使议定书仍不能生效.俄罗斯拒绝批准议定书的主要原因是本国的"经济效益"重于温室效应.虽然俄罗斯不批准议定书的行为,未构成国际不当行为,从而无须承担国际责任,但该行为在很大程度上与国际法的诚意原则的基本精神相抵触,是国际社会所公认的国际背信行为.     

劳资合作：入世后我国企业劳资关系的发展方向

入世将引起我国劳资关系的深刻变化,劳资关系的剧烈冲突将成为不争的现实。美国通用汽车公司实施的劳资合同战略为我国企业协调劳资关系提供了成功的经验。遵循“以职工为本”的管理思想,通过劳资双方的互动来加强劳资合作,应该成为我国企业协调和改善劳资关系的方向。