A practical method for the accurate determination of methemoglobin in blood containing carboxyhemoglobin

Kinetics of the oxidation of carboxyhemoglobin (HbCO) by potassium ferricyanide was studied photometrically in a weakly acid solution. An increase in the absorbance at 630 nm reached a maximum within 10 min when over a 100-fold excess of ferricyanide to hemoglobin iron was used. A slight decrease in the absorbance was observed after completion of the reaction when over a 500-fold excess of the reagent was used. In the presence of 0.4% Sterox SE, the absorbance began to decrease without complete oxidation. From these findings, a simple, rapid and accurate method for the determination of methemoglobin (Met-Hb) in blood was devised. The method was compared with two other methods, using 11 blood samples containing various amounts of HbCO, and proved to be suitable for blood containing elevated HbCO as well as for ordinary blood.     

Brazil as ‘Southern donor’: beyond hierarchy and national interests in development cooperation?

This article analyses Brazil's growing role in external development assistance. During Lula da Silva's presidency, cooperation with developing countries grew dramatically. While the official position is that Brazilian development assistance is moved not by national economic or political interests, but by international ‘solidarity’, and does not reproduce the North–South traditional aid relations, we suggest that it is not completely divorced from national, sub-national or sectoral interests and cannot be viewed apart from Brazil's broader foreign policy objectives. Brazil does pursue political, economic and commercial interests and, concomitantly, has made a positive difference in the recipient countries. However, more empirical research and field investigation are needed to better gauge the impact of Brazil's assistance initiatives and their contributions to South–South cooperation more broadly. During Lula's terms (2003–2010), Brazil could be classified as a ‘Southern donor’, which expresses the country's own novelties, and tensions, of simultaneously being a donor and a developing country.     

Lessons Learned from Autopsying an Unidentified Body with Iodine‐125 Seeds Implanted for Prostate Brachytherapy

We report here lessons learned from an autopsy case involving radioactive materials. We performed an autopsy of an unidentified mummified man with no available medical history whom from imaging findings we suspected had received radioactive seed implants for prostate brachytherapy. We returned the excised prostate and seeds to the body. A few days later, the body was identified by DNA matching and cremated. According to the man's medical record, he had undergone iodine‐125 seeds implantation for prostate cancer 11 months earlier. We should have removed the radioactive seeds from the body to prevent radiation exposure to the bereaved family and/or environmental pollution due to cremation. Surprisingly, one seed was found in the stored prostate specimen. Forensic experts should be cognizant of the risk of both radiation exposure in the autopsy room and environmental pollution. We must remain abreast of the latest advances in medicine.     

The Impact of Discontinuation of the Medical Examiner System: Cases of Drowning in the Bathtub at Home

The incidence of death by drowning greatly varies among different prefectures in Japan, mainly due to climate difference. However, there could be other factors affecting the incidence of deaths besides climate, for example, differences in regional death investigation systems. Here, we aimed to elucidate other such factors affecting the mortality data of drowning in the bathtub, especially the effects of discontinuing the medical examiner system. Police data in Kyoto and ambulatory care information in Yokohama were used. Data on cases of elderly individuals found dying or dead in the bathtub at home in winter 2014–2015 were obtained. The following data were collected for each case: age, gender, presence/absence of ambulatory transport, performance of autopsy, and cause of death. The autopsy and drowning rates in Kyoto were 0%, whereas both values in Yokohama were significantly higher at 93.1% and 89.4%, respectively (the denominator of each of the rates is the total number of elderly (aged 65 or over) individuals found dying or dead in the bathtub at home in each city during each winter). Despite no significant difference of incidence of total bath-related death, the proportion of drowning-related deaths was overwhelmingly higher in Yokohama than in Kyoto. The difference can be attributed to the difference in autopsy rates between the two cities, mainly caused by the presence/absence of a medical examiner system. Therefore, we should pay careful attention to future changes in autopsy/drowning rates in Yokohama, and ascertain whether the change might be continuously influenced by the abolishment of this system.     

Security Dependence and Asymmetric Aggressive Bargaining: North Korea's Policy toward the Two Superpowers

Abstract

This article addresses why small powers initiate aggressive bargaining with great power allies and adversaries despite the risk of provocation. Although the cause of such behavior is usually attributed to the regime type or the “irrationality” of an aggressive small power, this article explores how a system-level factor affects incentives for a small power to conduct aggressive bargaining. In so doing, I develop a theory of asymmetric aggressive bargaining, which shows that a small power's high security dependence upon its ally or adversary makes its use of aggressive bargaining rational. The empirical analysis suggests that the proposed theory effectively explains changes in North Korea's policies toward the United States and the Soviet Union after the Korean War.     

An Approach to Measuring Subnational Administrative Autonomy in Education

Efforts to measure decentralization have focused on political or fiscal autonomy, but not on administrative autonomy. Focusing on the education sector, we measured de facto subnational administrative autonomy across 64 countries/economies, via the domains of human resource management, salary management, budget management, and service content design. The findings reveal that subnational autonomy can differ substantially across those domains and within countries, and that the use of a single country-level indicator for administrative decentralization is inadequate. Furthermore, we show that administrative decentralization is as significant a factor as federal status in evaluating the degree to which a country/economy is decentralized or centralized.     

Stalinist Public or Communitarian Project? Housing Organisations and Self-Managed Canteens in Moscow's Frunze Raion

In 1929, when rationing was introduced widely across the Soviet Union, urban residents and housing organisations started to organise their own canteens at the residence level by collecting their ration books. These were called ‘self-managed canteens’ (samodeyatel'nye stolovye). Although this canteen project did not expand as expected during the food crisis of the early 1930s, the project was more successful in Moscow's Frunze raion. This was especially true of canteen No. 171, reportedly established at the initiative of housewives, which continued to operate until 1940 with a steady growth in gross sales, and which started to allocate part of its profits to local community activities. This unique case exemplifies a communitarian project by residents which might lead to a reconsideration of the ‘public’ under the Stalinist regime.     

Hyperuricemia in rats during acute carbon monoxide poisoning

The effect of carbon monoxide (CO) inhalation on plasma levels of uric acid and hypoxanthine in rats was investigated. Exposure to 3% CO caused respiratory arrest within about 2 minutes. The plasma uric acid level of CO-treated rats increased to 157% above that of ether-treated rats. When rats were exposed to 1% or 0.8% CO, the exposure periods until the onset of respiratory arrest were prolonged, and plasma uric acid levels at respiratory arrest were further elevated. Plasma uric acid levels at respiratory arrest increased with prolongation of the exposure periods. Under our experimental conditions, hypoxanthine or xanthine was not detected in plasma of CO-treated rats. These results are discussed in relation to the hyperuricemia in hemorrhagic shock or hypoxemia: CO-induced hyperuricemia can be attributed to the stimulated degradation of adenine nucleotides under tissue anoxia, and thus could be an excellent parameter of tissue anoxia.     

Immunoassay for methamphetamine with a new antibody

p- and o-Aminomethamphetamine were synthesized as haptens to be coupled with carrier protein at the benzene ring of methamphetamine. Immunogens were prepared by the glutaraldehyde method or the MBS (N-(m-maleimidobenzoyloxy)succinimide) type cross-linking reagent method. In particular, immunization with p-aminomethamphetamine-bovine serum albumin (BSA) conjugate prepared by the glutaraldehyde method gave an anti-methamphetamine antiserum having a low cross-reactivity with methylephedrine. With the antiserum, three kinds of immunoassays for methamphetamine were established. An enzyme immunoassay (EIA) and an enzyme-linked immunosorbent assay (ELISA) were developed with alkaline phosphatase (ALP) as a label enzyme. The amount of antibody bound ALP conjugate was determined by its activity in dephosphorylating p-nitrophenyl phosphate in EIA and nicotinamide adenine dinucleotide phosphate (NADP+) in ELISA. The range of methamphetamine measurable by ELISA was 0.025-0.5 ng/well and its sensitivity was superior to that of EIA (0.3-300 ng/tube). A latex agglutination inhibition reaction test (LAIRT) was also developed for the mass screening method of urine samples. The sensitivity of this method for methamphetamine was 0.1 micrograms/ml urine.