重组纤维连接蛋白EDA-EDB融合蛋白的分子克隆

目的构建重组pET28a-EDA-EDB质粒,制备重组纤维连接蛋白EDA-EDB融合蛋白。方法采用基因重组技术,将EDA、EDB基因片段连接,再将该重组基因插入pET28a表达载体,表达重组融合EDA-EDB蛋白后,利用6×His/Ni-NTA纯化系统进行纯化,免疫印迹法检测。结果成功连接EDA、EDB基因片段,重组pET28a-EDA-EDB质粒;在大肠杆菌BL21(DE3)中高度表达重组蛋白,获得较纯的重组蛋白,免疫印迹法证实为FN的组分。结论EDA-EDB重组蛋白能采用pET系统在大肠杆菌中表达,并可通过6×His/Ni-NTA纯化系统进行纯化而获得免疫原。     

建设社会主义和谐文化的若干原则

建设社会主义和谐文化是构建社会主义和谐社会的重要组成部分。我们所要建设的社会主义和谐社会,应该是民主法治、公平正义、诚信友爱、充满活力、安定有序、人与自然和谐相处的社会。建设社会主义和谐文化作为一项基础性工程,时间长,任务重,难度大,需要通盘考虑。为适应这一要求,在社会主义和谐文化建设中必须正确认识和着力把握好五个原则。     

增强自主创新能力 构建创新型城市

城市是经济社会发展的重要载体,构建创新型城市对于增强自主创新能力、转变经济增长方式、促进城乡统筹发展、推进城市自身进步、顺应世界城市发展趋势都具有十分重大的意义。创新型城市的构建要素可归结为产业创新、基础设施创新、制度创新、环境和文化创新、人才创新五个方面。而建设创新型城市,需要提升创新思维,弘扬创新文化;加强制度建设,完善创新机制;加速科技进步,提高创新能力;加强互动合作,注重成果转化;实施人才战略,打造人才高地。     

侦查阶段犯罪嫌疑人的权利保护

沉默权制度的引进对于犯罪嫌疑人的权利保护是积极的.然而,目前我国在侦查实践中仍严重存在刑讯逼供的现象.为切实有效地保障犯罪嫌疑人的权利不受侵犯,必须从制度上加以完善,从规则上加以明确,并通过加强法制宣传教育,增强侦查人员的法制观念,树立依法侦查、文明取证的侦查观.     

An end to a rumour

Mardin, is a city in the southeastern part of Turkey where people from different cultures and religions have been living together peacefully for centuries. The province hosted many valuable historical constructions representing different civilizations. Kasimiye Medresse, one of the most important educational centers of its times, has a sacred value for people in Mardin. The reason is that the stain on the wall of Kasimiye Medresse is considered to be Sultan Kasim's blood. Our study aims to analyze if the stain in question is blood. Serological tests are performed by using "Kastle-Meyer" and "Luminol" reactives on the scrapped samples taken from stained and unstained parts of the wall. At the end of the analysis, the stain is turned out to be a dye made of herbal roots ending the rumour of centuries.     

The identification of a dismembered human body: a multidisciplinary approach

A criminal case was directed to a multidisciplinary forensic team for identification, concerning a victim whose head, having two gunshot wounds, had been separated by a sharp instrument and was recovered 6 months later. The purpose of this research was to determine the sex and age of the victim for human identification. Primarily, macroscopic examination of the skull, tooth, and DNA analysis was conducted for sex determination. A rough assessment of age was made from the skull based on anthropological findings, however a more definitive result of age estimation was determined utilizing dental morphology. The dental data showed an age range of 32-37 from the mineral examination and the formulation of microscopic measurements. The results obtained from the skull and dental analysis matched with the physical characteristics of the victim's body, the known personal data of this person, and with the superposition of the photos gathered by a formal request. Besides, the result of DNA profiling of the victim showed male gender and direct relationship with the victim's presumed wife and daughter. Generally, research on human identification consists of sex and age determination. The sex characteristics can be precisely proved from DNA tests. However, age can be estimated by skeletal, and dental analysis. In this case the performed sex and age analysis lead the research to the selective matching of the missing person's identity.     

血中碳氧血红蛋白饱和度测定影响因素的研究

目的 考察血中碳氧血红蛋白饱和度(HbCO％)测定的影响因素,为其结果评定和所需样品保存条件提供实验依据。方法 利用三种分光光度法,测定30d内不同条件下保存的CO阳性血的HbCO％的变化。结果 还原双波长法、双波长法测定结果比较稳定,单波长法抗干扰能力较差;尸检所取血样的保存条件包括温度、保存时间及与空气接触程度对HbCO％的测定均有影响,其中温度影响较为显著。结论 利用还原双波长法与双波长法,并结合光谱扫描观察峰形变化可得到比较可靠的结果。30d内4℃条件下,密闭容器中血样接触少量空气不影响其HbCO％的测定。     

韩国行政规制基本法的制定与实施

韩国行政规制基本法的制定与实施@李秀峰$中国青年政治学院!北京100089 @陈晔$中国青年政治学院!北京100089~~     

外资收购"债转股"的若干法律问题思考

当前,债转股已成为国企改革的一项重大措施,外资收购"债转股"不失为一项可取的出路.但是,由于我国在公司法、外资法等法律中存在一定问题和矛盾,以及欠缺反垄断立法等方面的原因,以及外资收购行为中主体安排的缺陷,将对外资顺利收购"债转股"及收购后企业待遇、有关的国家监管等方面产生一定法律上的困惑和问题,有待相关机关对相应的法律进行修改、整和,并加紧进行相关产业监管立法,以尽早解决这些法律问题.