Toward maintaining canine training aid integrity: Visualizing contamination and testing storage materials for a non-hazardous canine training aid

Contamination of canine training aids is a pervasive issue that may lead to incorrect canine discrimination of target odors. It is therefore important to properly store training materials to maintain their integrity and efficiency. First, this study demonstrated the potential for contamination using GloGerm™ as a proxy for odor/particulate transfer. Then, eight types of containers were evaluated to determine (1) the ability to prevent odor permeation and (2) the likelihood of maintaining the ab/adsorbed odor. Lastly, a longitudinal study evaluated how the permeation of the target odor changed over time. Analysis occurred using a direct analysis in real-time mass spectrometer (DART-MS) to detect triacetone triperoxide (TATP) from the non-hazardous canine training aid known as the polymer odor capture-and-release (POCR) system. Results showed that Mylar and Opsak bags were most effective for short-term storage, maintaining low levels of ab/adsorption. Over time, the amount of TATP permeating through the primary containers and collecting in a secondary container (i.e., outer packaging) increased at 1 week and decreased thereafter (up to 4 months). The amount of TATP collecting in the primary containers, however, increased up to 1 month and decreased thereafter.     

Policy Response to Declining Birth Rate in Japan: Formation of a “Gender-Equal” Society

Japanese women have been assigned to the private role of caretaker, but the Japanese government has made prominent efforts in constructing a “gender-equal” society during the past decade. This policy development has come under the context of falling birth rate. The Basic Law for a Gender-Equal Society and the measures taken by the government so far still fall short from enforcing gender equality and do not affirm equality as a human right. Since the pursuit of gender equality is a means to boost the birth rate, when there is a contradiction between these two goals, the former will be conceded.

Field-portable detection of fentanyl and its analogs: A review

The need to detect fentanyl and its analogs in the field is an important capability to help prevent unintentional exposure or overdose on these substances, which may result in death. Many portable methods historically used in the field by first responders and other field users to detect and identify other chemical substances, such as hazardous materials, have been applied to the detection and identification of these synthetic opioids. This paper describes field portable spectroscopic methods used for the detection and identification of fentanyl and its analogs. The methods described are automated colorimetric tests including lateral flow assays; vibrational spectroscopy (mid-infrared and Raman); gas chromatography–mass spectrometry; ion mobility spectrometry, and high-pressure mass spectrometry. In each case the background and key details of these technologies are outlined, followed by a discussion of the application of the technology in the field. Attention is paid to the analysis of complex mixtures and limits of detection, including the required spectral databases and algorithms used to interrogate these types of samples. There is also an emphasis on providing actionable information to the (likely) non-scientist operators of these instruments in the field.     

Rapid enantiomeric analysis of zopiclone in serum by supercritical fluid chromatography–tandem mass spectrometry

Zopiclone (ZOP) is a hypnotic drug prescribed to treat insomnia. Due to the chiral nature of ZOP, the psychologically active S-form and inactive R-form need to be determined enantiomerically in a forensic drug analysis. In the present study, a supercritical fluid chromatography (SFC) method was designed with a faster analysis ability than that of previously reported techniques. The SFC–tandem mass spectrometry (SFC–MS/MS) method was optimized using a column with a chiral polysaccharide stationary phase (Trefoil CEL2). ZOP was extracted from pooled human serum using solid-phase extraction (Oasis HLB) and analyzed. The developed SFC–MS/MS method achieved the baseline separation of S-ZOP and R-ZOP within 2 min. The fit-for-purpose method validation indicated that the optimized solid-phase extraction achieved near complete recovery and approximately 70% of the matrix effect. Both the retention time and peak area showed sufficient precision. The lower and upper limits of quantification (LOQ) were 5.7 × 10⁻² ng/mL and 25 ng/mL for R-ZOP, and 5.2 × 10⁻² ng/mL and 25 ng/mL for S-ZOP. The calibration line was linear in the range from lower LOQ to upper LOQ. The stability test indicated that ZOP in serum stored in a refrigerator (4°C) degraded and about 55% remained in 31 days. The quick analysis of the SFC–MS/MS method makes it a valid option for the enantiomeric analysis of ZOP.     

Explaining Australia–Japan security cooperation and its prospects: ‘the interests that bind?’

In this paper, we assess the drivers of, and prospects for, further security cooperation in the Australia–Japan bilateral relationship. We argue that while balance of threat and capability gaps may restrain the pace of deepening Australian-Japan security relations, these gaps are diminishing and are, in any case, secondary to the specific shared interests we posit as the primary drivers of Australia–Japan security cooperation. These specific interests, however, are shaped not only by a commitment in both countries to liberal-based principles and freedoms at home and in inter-state relations, but also by a common awareness of the importance of continued US primacy to the ongoing maintenance of those interests. Thus, in the absence of any fundamental change in how Australia and Japan understand their respective interests and the US role in the region, Donald Trump's recent and unexpected election as US president notwithstanding, we conclude that the case for further security cooperation – via additional cross-bracing of their respective US alliance relationships – will almost certainly remain compelling for both countries.     

Great East Japan Earthquake: Lessons Learned for Japan's Defense Posture

Experience in the Great East Japan Earthquake (GEJE) by the Japan Self-Defense Forces (JSDF) was unprecedented in many ways. In addition to being the largest mobilization in its history, it was the first time that the JSDF operated under the Joint Task Force (JTF); it was also the first time that the JSDF was faced with a complex disaster that included both a large-scale natural disaster and the worst nuclear accident in Japan's history. The JSDF took away a number of “lessons learned” from this experience. These “lessons learned” by and large validate the “dynamic defense force” concept in the December 2010 National Defense Program Guidelines. Whether they can capitalize on the GELE experience and move forward is up to the JSDF and MOD leadership.     

From immigrants to stayers: Micro-demography of a historical lineage in Sichuan,China

This paper studies the demographic profiles of Chinese domestic immigrants during the Qing era, using the genealogy of one lineage in Sichuan, Yunyang Tu-shi Zupu. The genealogy records individual life history beginning with the immigrant ancestors to Sichuan. This study mainly uses the vital dates recorded in each member's individual entry to the genealogical table, analysing change in demographic indices over time and their causal relationship with individual/family characteristics. The micro-demographic analyses show that the fertility and nuptiality were initially high but on the decline over time while the mortality might have followed the opposite path. This result indicates that the founding generations of the lineage enjoyed a relatively rich life but living standard gradually deteriorated as population pressure on food production became strong. The differential of the initial and terminal generations can be regarded as that of immigrants and stayers. The demographic trend reported in the study shows the assimilation process of immigrant lineages to natives. The higher population growth rate of immigrants derived in this argument may help to mitigate the controversy of the high population growth during the Qing and the low fertility estimates obtained from sedentary populations in the previous studies.     

Radiographic age estimation based on degenerative changes of vertebrae

Age estimation is an important component of decedent identification. When assessing adult remains, anthropologists frequently use gross examination of skeletal elements, such as clavicles, ribs, and pubic symphyses. For fleshed bodies, this requires the removal of these elements and maceration prior to analysis. A new method was developed using radiographic imaging to estimate age from degenerative changes of the lower thoracic and upper lumbar vertebrae. This technique will complement anthropological age estimation methods in young and middle-aged adults and may serve as a stand-alone method for older individuals. Digital radiographs from 240 medical examiner cases were evaluated. The sample included 120 females and 120 males between the ages of 18 and 101 years. A 3-phased scoring system was used for the target vertebrae. Transition analysis was conducted on binned average scores and a Bayesian approach was used to assign age intervals. At the 90% credible interval, individuals in Bin 1 were under 36 years of age while those in Bin 3 were over 47 years of age. Individuals in Bin 2 showed too much age variation to be informative. No significant differences were found between males and females. These findings will be especially useful in the age estimation of older adults and may eliminate the need for skeletal sampling in medicolegal cases where advanced degenerative changes are radiographically observed in the lower thoracic and/or upper lumbar vertebrae. This method was developed for use on fleshed individuals but may also be applicable to skeletonized remains.