急性心肌缺血再灌FOS免疫组织化学实验研究

探寻FOS蛋白在心肌早期缺血再灌流损伤中变化规律,为心性猝死的诊断提供新方法。利用SD大鼠建立心肌缺血再灌流损伤模型,设立正常、缺血对照组与缺血再灌组。心脏标本经HE染色及免疫级化观察。结果发现,在冷冻切片上缺血20min再灌流30min,再灌流区心肌细胞核呈阳性着色。但在石蜡切片上,缺血30min再灌流30min后,再灌流区才有心肌细胞核（37．76％±9．66％）呈弱阳性着色,再灌流60min后核呈棕褐色阳性染色,120min后开始减弱（35.36％±3.16％）。正常和单纯缺血组心肌细胞核未见有阳性反应。HE染色无明显病理改变。结果提示,SABC－FOS免疫组织化学方法最早可揭示心肌缺血20min再灌流30min的损伤,FOS蛋白在再灌流后60－120min之间可能有一个高峰表达。此法对显示实验性心肌早期缺血再灌流损伤有重要的价值。有望用于心性猝死的诊断。     

甲醛固定及石蜡包埋组织的DNA分型及其应用

目的：建立甲醛固定及石蜡包埋组织ＤＮＡ的基因分型方法并使之应用于实际检案。方法：用Ｃｈｅｌｅｘ和有机溶剂提取法提取ＤＮＡ,以ＰＣＲ与反向探针杂交技术作ＰＭ和ＨＬＡ－ＤＱＡ１位点分型。结果：２６份包埋组织块有２４份能得到理想的分型结果。结论：本法可用于法医学个人识别和亲子鉴定     

肾上腺结核猝死的法医学鉴定

通过两例肾上腺结核猝死的案例观察发现：肾上腺和肾上腺以外器官的结核病变均表现为不典型的慢性陈旧性的病理学特征。提示在法医学鉴定中,对患过结核病和“不明死因”猝死尸体剖验时肾上腺的组织病理学检验尤为重要     

中国知识经济与区域发展布局新思维

文章以知识经济理论和新区域发展理论为指导,构造了知识经济和区域发展的杠杆模型,坚持以人为本和科学发展观,提出按中国经济的三元结构——“三元中国(信息中国,工业中国,农业中国)”划分考察国情,以“升岛成陆”战略发展中国知识经济和区域经济,从变革行政区划入手进行制度创新,加快知识化(信息化)、城市化、市场化,在根本上解决区域不平衡、失业、可持续发展等问题。     

Haemorrhages in head tissues during the asphyxiation process.

Two rates of carbon dioxide flow into the termination chamber were applied for the euthanasia of rats. The classical phases of asphyxia were observed in both groups of animals although their course was prolonged in rats exposed to the lower gas flow. The haemorrhages were assessed histologically in the following structures: the pericranium, compact bone and diplo? of the skull, sagittal suture, nape muscle and its attachment, and the dura.The haemorrhages appeared regularly in the sagittal suture in all cases from both experimental groups. The possible significance of this phenomenon for the forensic practice was pointed out.     

A Non-Essentialist Version of Legal Pluralism

The concept of legal pluralism has been touted by many socio-legal scholars as a key concept in the analysis of law. Yet, after almost twenty years of such claims, there has been little progress in the development of the concept. This article will argue that the underlying cause of this lack progress lies in the fact that promoters of the concept have relied upon function-based, essentialist concepts of law. It will describe the problems generated by such concepts and, following this general analysis, will review the versions of legal pluralism articulated by Boaventura de Sousa Santos and Gunther Teubner. The critique of their versions of legal pluralism will lead into the posing of a non-essentialist alternative which avoids the conceptual problems of prevailing versions of legal pluralism, and provides a better tool for purposes of research and analysis of the relationship between law and society.     

The theory of cardiac lesions from blunt chest injury

The main theories of myocardial lesions associated with a blunt chest injury proposed starting from the XIXth century till the present time are considered based on the overview of the literature data. It is shown that the theory of selective mechanical activation of ATP-dependent K+ channels is most promising for further investigations into the mechanisms of myocardial dysfunction resulting from blunt chest injuries. The authors emphasize the absence of the universally accepted theory explaining the mechanism behind traumatic cardiac troubles and its fatal outcome despite numerous studies of cardiac lesions in patients with a blunt chest injury. It dictates the necessity of further research, both clinical and experimental, for a deeper insight into the problem.