口蹄疫病毒VP2基因的真核表达及其产物抗原性的检测

利用PCR技术,扩增出口蹄疫病毒(FMDV)VP2基因,并克隆到杆状病毒转移载体pBlueBacHis2A上。用重组质粒pB-VP2与重组病毒同时转染sf9昆虫细胞,获得了重组病毒。经过蚀斑筛选纯化后,感染sf9细胞,表达VP2融合蛋白,分子质量为33 ku左右。以牛抗O型FMDV血清为第一抗体,通过Western-blotting和Dot-ELISA鉴定,说明VP2基因在真核表达系统中获得正确表达,且可以与牛抗O型FMDV血清发生特异性反应。     

网络游戏外挂程序法律政策问题研究系列之三——外挂程序相关法律关系分析

本文着重讨论网络游戏（以下简称“网游”）开发商与外挂开发商之间、网游运营商与外挂开发商之间、网游运营商与玩家之间、国家与外挂开发商之间这四个方面的法律关系。由于外挂开发商与外挂经销商往往是同一个主体，所以对外挂经销商传播外挂的行为不单独进行讨论。     

Teenage suicide in King County, Washington. I. Rates of suicide for 26 years

The population-adjusted rate of teenage suicide in King County, Washington, was reviewed for the 26 years from 1959 to 1984. For the entire period, the rate did not change significantly. An abrupt change did occur during the middle of this period, but, for reasons discussed in the text, this may well represent a change in procedures and philosophy of death certification rather than a real change in rate. Reasons for the apparent discrepancy with national statistics are discussed.     

面向东南亚企业并购技术系统整合风险分析

随着中国—东盟自由贸易区正式启动,越来越多的中国企业看到东盟的无限商机,如何“走出去”在东南亚市场获得发展,是目前国内很多企业思考的问题。由于缺乏海外经营人才和经验,对东南亚市场行情及发展趋势也了解不足等因素,不少企业选择的是采取兼并当地原有企业的投资方式进行投资,比如海尔集团东南亚子公司大多是采取并购方式。这种方式与在东道国建设新厂或采用独资方式相比有许多优势:首先,可以缩短项目建设周期,减少资本支出,迅速扩大企业生产和经营规模;其次,可以直接占有被兼并企业原有市场,并利用其销售系统;再次,可以获得企业发展…     

推动农民生产生活方式根本性转变有效保护林草植被资源和生态环境

目前,我国生态环境局部改善、总体恶化趋势尚未根本扭转,其中一个重要原因就是当地农民不合理的生产生活方式没有真正转变,许多林草资源尚未得到合理开发利用和有效保护。因此,必须加快建设资源节约型、环境友好型社会,推动农民生产生活方式根本性转变,有效保护林草植被资源和生态环境。     

Spatial variation of refractive index in a pane of float glass.

In the statistical interpretation of forensic glass evidence it is standard practice to make the assumption of homogeneity of the refractive index (RI) of the source glass, or of localized homogeneity. However, the work of Locke and Hayes showed that, for toughened windscreen glass, this assumption might not be true. This work is well cited, but there appears to have been little follow-on published research. Furthermore, the toughening process is something known to affect the refractive index, and is a process that float glass does not undergo. Float glass is a major component of casework in New Zealand and for that reason it would be interesting to know whether the findings of Locke and Hayes apply when dealing with float glass. In this paper we describe an experiment similar to that of Locke and Hayes, systematically examining the variation of RI in a pane of float window glass. It was found that, although there were no systematic differences in refractive index, there were observable differences across the pane.     

A statistical approach to drug sampling: a case study.

In many countries it is left to the discretion of the court to accept or reject conclusions based on sampling procedures as applied to the total drug exhibit. As an alternative to this subjective approach, a statistical basis is presented using binomial and hypergeometric distributions to determine a lower limit for the proportion of units in a population which contains a drug, at a given confidence level. A method for calculating the total weight of a drug present in a population within a given confidence interval is also presented. In the event of no failures (all units sampled contain a drug), a sample size of six or seven units is generally sufficient to state that a proportion of at least 0.70 of the population contains a drug at a confidence level of at least 90%. When failures do occur in the sample, point estimation is used as the basis for selecting the appropriate sample size.