Determination of MDMA, MDA, MDEA and MBDB in oral fluid using high performance liquid chromatography with native fluorescence detection

This paper describes the analytical methodology for the determination of MDMA, MDA, MDEA and MBDB in oral fluid. After a liquid–liquid extraction, the analysis was carried out by high performance liquid chromatography (HPLC), with fluorescence detection. The detector wavelength was fixed at 285 nm for excitation and 320 nm for emission. The mobile phase, a mixture of phosphate buffer (pH = 5) and acetonitrile (75:25), and the column, Kromasil 100 C8 5 μm 250 mm × 4.6 mm, allowed good separation of the compounds in an isocratic mode in only 10 min. The method was validated and showed good limits of detection (2 ng/mL) and quantitation (10 ng/mL) for all the amphetamine derivatives. No interfering substances were detected. A stability study of these compounds in oral fluid stored at three different temperatures (−18, 4 and 20 °C) over 10 weeks was conducted, showing a time-dependent degradation of the four compounds.     

Can Business Development Services practitioners learn from theories of innovation and services marketing?

Business Development Services (BDS) programmes have become big business for international donors and NGOs. Focusing on small enterprises in developing countries, the current BDS approach assumes that the development of commercial markets is the key to success. Yet many of these programmes continue to have a limited impact. A review of modern theories of innovation and services marketing management suggests that this may be because current BDS support practice reflects a rather limited understanding of how new markets actually develop. Drawing on the insights that these theories offer, the authors suggest that BDS practice should develop a more evolutionary approach, recognising that service innovations develop through active, on-going interaction between suppliers and customers. The article concludes with practical policy guidelines and a discussion about tools that could help BDS to adopt this more successful approach.     

The zinc test as an alternative for acid phosphatase spot tests in the primary identification of seminal traces

The value of the acid phosphatase spot test in the primary visualization and identification of seminal traces is hampered by the sensitiveness of the enzyme to biodegradation. An alternative spot test is proposed, based on the high concentration of the more stable zinc metal in seminal plasma. The proposed zinc spot test is simple and suitable for on site investigation. Although the sensitivity in fresh stains is lower than that of the acid phosphatase spot test, this is largely compensated by the lower sensitiveness to biodegradation. The specificity for semen is higher than that of the acid phosphatase spot test. In vaginal swabs it was nevertheless seen, that samples should be taken within 24 h after alleged sexual assault to give reliable results.     

Evaluation of microbial soil identity in forensic science

Microbiologic analysis of different soil samples was undertaken to assess the value of this method for forensic purposes. Three groups of micro-organisms were isolated on selective media: bacteria, fungi, and actinomycetes. Comparison of the morphology of the colonies led to the conclusion of non identity of the soil samples under examination. The method was applied to soil traces on articles of clothing, trousers, shoes, and stockings. It was found that the soil did not originate from the suspected scene of a crime. Examination of the microbiologic soil composition does not provide an absolute certainty of identity, but it is highly reliable.     

Hydrolysis of clonazepam, flunitrazepam and nitrazepam by hydrochloric acid. Identification of some additional products

Several by-products in the hydrochloric acid hydrolysis of clonazepam, flunitrazepam and nitrazepam are isolated by a combination of TLC and HPLC. With mass and NMR spectroscopic methods the structures of the by-products are established. Some indications are given for possible modes of formation of the by-products.