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Nondestructive Methods for Recovery of Biological Material from Human Teeth for DNA Extraction 下载免费PDF全文
Montserrat Hervella Ph.D. Maitane G. Iñiguez M.Sc. Neskuts Izagirre Ph.D. Alberto Anta Ph.D. Concepción de‐la‐Rúa Ph.D. 《Journal of forensic sciences》2015,60(1):136-141
The extraction of DNA from human skeletal remains applied to forensic, and evolutionary studies do not exclude risks, which are to be evaluated when working with unique specimens that could be damaged or even destroyed. In the present study were evaluated several nondestructive methods for recovering DNA instead of the most currently used pulverization method. Three different procedures to access inside the dental pieces (occlusal perforation, cervical perforation, and cervical cut) have been compared with the aim of recovering as many cell remains as possible to carry out a DNA extraction. Given the DNA quantitation results, a method was proposed that consists of a cervical cut to facilitate the access to the pulp cavity and a subsequent filing of the root canals down to the apex of the dental root. This methodology allows the recovery of both mitochondrial and nuclear DNA, with the minimum deterioration for the dental pieces. 相似文献
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Hans van de Ven 《Intelligence & National Security》2013,28(4):205-237
The arrest in May 1942 of Liao Chengzhi – a CCP spy chief as well as the son of a celebrated KMT elder assassinated in 1925 – is considered a Big Case in China. Using the original KMT case file on Liao Chengzhi, van de Ven describes how the KMT's secret service turned the Jiangxi Provincial Committee and then arrested leading members of the CCP in south China and thereby eliminated its organization there. The article further uses letters by prominent CCP and KMT personalities involved in the case to suggest the existence of a revolutionary aristocracy bound by personal relations and with an elite code of conduct. When the political will was present, members of this elite deployed their personal connections in the search for solutions to complex political problems. 相似文献
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Suzanne de Brunhoff 《Economy and Society》2013,42(4):421-430
The fundamental distinction between Ricardo and Marx is once more examined, chiefly with reference to the current spread, among some leftwing economists, of a neo-Ricardian school. So a crucial point seems to be the particular meaning, which is quite a-Ricardian, of the first section of the Capital. Anyhow, this section is often misunderstood, because of its difficulty, and its philosophical form. 相似文献