Induction of hypoxia-inducible factor-1alpha in two kinds of rats asphyxiation death models

OBJECTIVE: To investigate the expression of hypoxia-inducible factor 1-alpha (HIF1-alpha) in the heart, lung, liver and kidney in rats died of two typical models of asphyxia. METHODS: Two asphyxia models were made and tissue samples of the dead rats were collected from different groups at various postmortem duration. The expression and the changes of HIF1-alpha in various tissues were examined by immunohistochemistry and image analysis techniques. Results Significant expression of HIF1-alpha was observed in the myocardial fibers, kidney cells, liver cells and lung cells in both asphyxia models, but not in the control group. The expression of HIF1-alpha in various tissues in the rat died of nitrogen gas breathing was found in the nuclei at 0 hour and the expression level decreased gradually thereafter. The HIF1-alpha expression level and duration in various tissues of the rat died of hanging were higher and longer than that of the former group, with a peak of the expression level observed 6 hours after death, and then started to decline in all tissues except the heart where the expression still showed an increase 24 hours after death. The control groups showed a steady expression in the cytoplasm but not in the nuclei. CONCLUSION: HIF1-alpha appears to be a valuable biomarker in the diagnosis of asphyxia within 24 hours after death.     

马克思主义哲学中国化研究的视野与方法

马克思主义哲学是被实践证明了的科学的世界观和方法论,马克思主义哲学中国化进行的好与不好,顺利或不顺利,直接关乎到马克思主义在中国能否实现真正的中国化———富有中国作风和中国气派,反映民族性和时代性。当前马克思主义哲学中国化过程中面临的种种困境和挑战,为一种新的研究视野与研究方法的提出提供了可能性。站在世界历史的视野,采用历史主义研究方法,马克思主义哲学中国化研究这种在视野与方法上的创新,有助于进一步推动马克思主义中国化。     

统一多民族国家的宪制建构——新中国成立初期民族区域自治制度的奠基历程

对于民族区域自治制度在我国的确立,《共同纲领》的宪政宣示尽管具有最为重要的合法性确认意义,但这一基本宪政制度的真正奠基,则有赖于民族识别、民族干部培养、少数民族地方民主改革等一系列相关政治实践。这其中,少数民族地方民主改革可视为我国民族区域自治制度奠基的关键,它直接决定了新中国的民族治理制度是否坚持和贯彻了社会主义方向这一民族区域自治制度的最实质所在。同时,新中国通过将民族区域自治制度作为我国民族治理的基本宪政制度,也实现了清末以来多民族大国的民族—国家建构的真正飞跃。     

Determination and validation of tetrodotoxin in human whole blood using hydrophilic interaction liquid chromatography-tandem mass spectroscopy and its application

A sensitive analytical method was developed for the quantitative determination of tetrodotoxin (TTX), a powerful sodium channel blocker, in human postmortem whole blood. The sample mixture was cleaned up using cation exchange SPE catridge after protein precipitation by methanol and then separated on a PC-HILIC (phosphorylcholine hydrophilic interaction liquid chromatography) column (150 mm × 2.0mm i.d., 5 μm) using a isocratic elution of 1% acetic acid and acetonitrile. The identification of TTX was performed on tandem mass spectrometry with electrospray ionization interface in positive ion mode. The retention time of voglibose (internal standard) and TTX was 5.1 and 6.0 min, respectively. TTX and internal standard (voglibose) were monitored and quantitated using the ion transitions: the respective precursor to product ion combinations, m/z 320/302 for TTX and m/z 268/92 for voglibose in the multiple reaction monitoring (MRM) mode. The recovery of TTX and voglibose was 61.4% and 62.8%, respectively and the good accuracy (97.7-103.9%), linearity (2-1200 ng/mL) and reproducibility were shown in this method. The limit of detection and limit of quantification were 0.32 ng/mL and 1.08 ng/mL, respectively. This method was applied in the case of three fishermen who were poisoned (including one death) by unknown fish on their boat in October 2010. In this case, the levels of TTX were 27.2, 30.0 and 29.7 ng/mL in heart blood, peripheral blood and serum of a victim, were 3.1 and 12.1 ng/mL in peripheral blood and 3.9 and 12.8 ng/mL in serum of two survivors, respectively.     

硫磺制模法提取雪地足迹的优势

目的比较硫磺制模法与石膏制模法提取雪地足迹的效果。方法分别用两种制模法实验,观察比较操作流程、制模时间、细节特征反映等方面。结果硫磺制模法在雪地足迹提取的速度、细节特征反映等方面优于石膏制模法。结论与传统的石膏制模法相比,硫磺制模法更加迅速、简便,在细节特征的反映更加清晰。     

内地西藏班(校)学生的跨文化适应

跨文化适应是指身处异文化环境中的个体或群体有意识、有倾向地调整自己的心理活动和行为方式,以便更好地融入异文化的过程,分为社会文化适应和心理适应两个维度。前者以学习适应、生活适应和交往适应为标志;后者以主观幸福感和心理健康为指标,内地西藏班(校)学生的跨文化适应状况是喜少忧多,在诸多方面适应不良。造成这种状况的根源是文化中断。从外部加强社会支持、缩短文化距离,消除歧视与偏见;从内部确立合理的评价与应对方式,增加与文化相关的知识技能,是应对跨文化适应不良的有效措施。     

Doing More in The Middle East

正China becomes increasingly involved in Middle East issue The international community once described China’s Middle East diplomatic policy using phrases such as"modera-tion,""detachment,"or"lack of imagination."But as its status as a global player grows,China is no longer merely a distant observer of events in the volatile region.