Direct amplification of STRs from blood or buccal cell samples

Forensic databasing laboratories routinely analyze blood or buccal cell samples deposited on FTA^® paper. Prior to PCR amplification of the STRs, the FTA^® samples must undergo multi-step sample purification protocols to remove the PCR inhibitors present within the sample and from the FTA^® paper. The multi-step sample purification protocols are laborious, time-consuming and increase the potential for sample cross-contamination.To eliminate the need for DNA purification, we conducted studies to optimize the PCR buffer and thermal cycling parameters to allow for direct amplification of STRs from blood or buccal samples on FTA^® paper. We evaluated the effect of various factors on the DNA profile including: FTA^® disc size, blood sample load variation, and buffer formulation. The new STR assay enables the direct amplification of DNA from single source samples on FTA^® discs without sample purification. The new STR assay improves the workflow by eliminating tedious steps and minimizing sample handling. Furthermore, the new STR assay reduces cost by eliminating the need for purification reagents and expensive robots.     

我国收入分配差距快速扩大问题探析

收入分配差距过大,不利于经济发展与消费增长,影响民族团结,动摇党的执政基础,增加改革的成本,引发信仰危机.政府须加快税法建设,调节高收入;加快工资制度改革,扩大中等收入阶层;加快法律制度建设,取缔非法收入;加快取消束缚农民的制度,加大对农民、农业、农村的支持力度.加快调整财政支出结构,增加社会保障支出;加快市场法制化规则化,完善市场体系等,以缩小收入分配差距,促进社会和谐.     

论已确认事实的预决力

已确认事实的预决力既不同于裁判的既判力,也有别于"争点效"和"争点排除规则",其是我国民事诉讼法确立的一项具有独特内涵的制度。然而,由于我国民事诉讼法未明确预决力的条件和范围,从而致使司法实践中法院的做法各不相同,这种状况一方面有损法院判决的一致性、权威性,也与确立预决力制度的初衷相违背,因此,民事诉讼法的修改应明确预决力的条件和范围。实践中,法院应根据已确认事实的不同具体认定其不同的预决力。     

The suicide mortality rates between 1997-1998 and 2000-2001 in Nantou County of Taiwan following the earthquake of September 21 in 1999.

The paper aims to show the different suicide mortality rate between 1997-1998 and 2000-2001 in Nantou County of Taiwan with earthquake on September 21 (often referred to as the "9-21 earthquake") in 1999. In additional, it also identifies the preventive strategies for the high-risk suicide population. The age-standardized mortality rates for suicide in Nantou County were calculated for the years 1997-1998 and 2000-2001. The suicide standardized mortality ratio (SMR for townships in Nantou were calculated by "type of township" as the standard rate). There is a statistically significant difference in male suicide rates for the years prior to the earthquake (1997-1998) when pooled and compared to the suicide rates for the years after the earthquake (2000-2001). The rate for four age groups (under 25, 25-44, 45-64, 65 and above) all increased, yet all but one (the group of age 45-64) was not statistically significant. The male SMR has slightly increased after the 9-21 earthquake. Yet there are no statistical significances in most townships, except in Kaohsiung and Puli after the 9-21 earthquake. The Kaohsiung SMRs were 1.36 (95% CI: 0.54-2.80) before the earthquake (1997-1998) and 2.01 (95% CI: 1.04-3.52) after the earthquake (2000-2001). The SMRs before and after the earthquake in the Puli Township were 1.51 (95% CI: 0.95-2.29) to 1.56 (95% CI: 1.03-2.27). This study suggests that monitoring high-risk population, especially males or 45-64 years of age who experienced the highest statistically significant suicide rate in this study. The study provides support for providing both the psychological restoration program and, to the extent feasible, financial support for the unemployed as useful public health strategies for suicide prevention in Taiwan.     

鼠抗人转铁蛋白单链抗体基因的构建

目的为了得到可经载体连接表达、并只由抗体可变区构成的单链抗体基因而进行本实验.方法经扩增得到的鼠抗人转铁蛋白抗体轻、重链可变区基因经凝胶电泳分离、离心柱纯化及定量,同能柔性折叠的一段短连接肽基因一起进行扩增拼接反应,而后在含限切酶位点的引物指导下扩增引入限切酶SfiI和NotI的酶切位点识别序列,产物以电泳鉴定、纯化和定量,再分别用SfiI和NotI酶切消化,最后再柱纯化.结果得到了携带可与载体连接的粘性末端的鼠抗人转铁蛋白单链抗体基因.结论两个不同的基因通过两端含互补序列的第三个基因片断可只经过扩增而无需DNA连接酶的作用而连接起来.对于抗体基因可由此而产生新的轻重链基因的组合,有可能产生性能更优异的抗体.     

华蟾素配伍丝裂霉素体外抑瘤作用

目的：从体外药敏实验的角度观察华蟾素注射液配伍丝裂霉素对不同肝癌细胞抑瘤效果和对正常肝细胞的毒性反应。方法：通过四甲基偶氮唑盐比色法观察低浓度华蟾素注射液、丝裂霉素及两配伍使用时对人肝癌细胞系SMMC-721、BEL—7402细胞及正常肝细胞系L-02细胞的生长抑制作用。结果：对人肝癌细胞的生长抑制作用，华蟾素及丝裂霉素两配伍使用较两单独使用显增强；丝裂霉素对正常肝细胞具有一定生长抑制作用，而华蟾素注射液则无此作用，与单独使用丝裂霉素比较，两的配伍使用对正常肝细胞的生长抑制作用未见明显增加。结论：华蟾素和丝裂霉素配伍使用可显提高对人肝癌细胞的杀伤作用，同时未增加对正常肝细胞的生长抑制作用，提示华蟾素和丝裂霉素配伍使用在肝癌治疗中具有很好的临床应用前景。     

数学学习困难儿童的认知加工机制研究

与数学学习优秀儿童相比,数学学习困难儿童的语音加工速度、短时记忆、中央执行功能以及整体工作记忆能力方面都存在明显不足,但只有工作记忆能力不足才能明确解释数学学习困难,即数学学习困难与工作记忆能力下降有密切关系.进一步分析发现,由工作记忆能力下降引起的数学学习困难与特定的工作记忆能力不足有关,即数学学习困难几乎完全是由于数字工作记忆能力下降引起,而与视空间工作记忆能力下降无关,这种由数字工作记忆下降而引起数学学习困难的更深层次原因可能在于较差的语音加工速度与中央执行功能二者的共同影响.     

使大学更像大学——现代大学与政府关系的一项重要原则

大学教育作为传播和创造人类明的事业其精神和品格无疑是高贵且神圣的然而这种作为大学特征的高贵性和神圣性，在我们现实的大学中却显得淡薄且逐渐被疏远，而与此精神相悖的功利主义，庸俗风气则日见昌盛，这无疑显现了大学中存在着深重的精神危机，要使大学显现出自身就有的高贵性和神圣性，就就该让大学像个大学，而大学更像大学的基本途径，就是对大学与政府之间行政性隶属关系的解构和致力于大学地自主办学的新制度创造。     

试论现代汉语核心句的句义结构类型

作为语法研究的一个有顶组成部分，句义结构的类型研究必须要遵循语形语义相结合的基本原则，本根据区别性语义特征将现代汉语基础表义结构分为属性，方们，领属，识别，感知，自身动作，涉客动作等七小类，并采用谓词逻辑对各小类基础表义结构的内部语义配置式进行描写，指出现代汉语句子的语义成分是一个逐层概括的层级系统，现代汉语核心锯的语义结构有五种基本类型。     

三代领导核心与黄河的治理开发

治理黄河是毛泽东牵肠挂肚的一件大事,他曾四次考察黄河.邓小平十分重视黄河的治理与开发,增加了黄河下游防洪工程费用,兴建小浪底水库.江泽民对黄河的治理与开发问题始终关注着,先后七次考察了黄河.