法国税法研究生教育对我国的启示

法学高等教育的人才培养目标是什么?是应用实践型的工匠,还是专注学术的大师?在实践中,我们往往将培养"法学大师"作为追求目标,而对"法学工匠"的教育嗤之以鼻.笔者认为:大师和工匠在推动中国法治建设的道路上同等重要,两者均具有独立存在的价值,他们在角色扮演中发挥的互动作用,为中国的法治进程起到了推波助澜的作用.大师不应以渊博深厚的法学功底而蔑视工匠,工匠也不应以精湛的诉讼技巧而轻视大师.     

日韩两国文化的比较研究

日韩两国拥有相近的习俗和文化,但又各具鲜明特色.本文分析了日韩两国文化的共性与个性,并剖析了两国文化差异的根源.     

围绕创新实现工会工作新突破

在创新中求活力、谋发展,是工会组织的优良作风。随着我国经济成分多元化、分配关系多样化、劳动关系契约化、劳动就业市场化等市场经济体制特征的日益显现,工会工作必须从观念上、制度上、重点工作上以及工作方法上实现创新,以适应形势发展的需要,更好地维护职工的切身利益。为构建以人为本、和谐文明的社会主义社会发挥积极作用。     

合同法中的附随义务探讨

附随义务是指合同关系发展过程中及合同关系终止后的一定时期,依诚实信用原则当事人所应负担的给付义务以外的义务。附随义务的理论基础来源于诚实信用原则。确立附随义务有利于平衡各方利益关系、强化对债权人的保护、维护社会秩序稳定及完善合同法立法与理论。附随义务内容随合同关系发展而有不同的体现。基于附随义务发生阶段的不同,违反附随义务的法律后果亦不同。     

浅论我国高等职业法律教育的几个基本问题

目前,我国高等职业法律专科院校的紧迫问题是生存问题,解决这一问题的路径就是在积累办学经验、提高办学水平和改善办学条件的基础上提升办学层次,由专科教育过渡到本科教育层次。本文在分析高等职业法律教育特征的基础之上,认为我国高等职业法律教育应主要定位在本科教育和法律硕士教育层次,大专层次法律教育随着我国高等法学教育的进一步发展,可以逐渐淡出法律职业教育市场。     

Simultaneous Determination of 13 Anticoagulant Rodenticidesin Human Blood by Liquid Chromatography–Tandem Mass Spectrometry and its Application in Three Poisoning Cases

Anticoagulant rodenticides are widely used for rodent control around the world. A rapid and sensitive method was developed and validated for the simultaneous determination of 13 anticoagulant rodenticides (coumafuryl, pindone, valone, warfarin, coumatetralyl, coumachlor, diphacinone, dicumarol, chlorophacinone, bromadiolone, difenacoum, flocoumafen, and brodifacoum) in human blood by liquid chromatography–tandem mass spectrometry. After liquid–liquid extraction, the anticoagulant rodenticides were separated on an Eclipse Plus C18 column. Linearities were observed for each analyte in blood ranging from 0.5 to 50 ng/mL, with correlation coefficients over 0.99. The limits of detection ranged from 0.01 to 0.2 ng/mL, and the limits of quantification were 0.5 ng/mL for all analytes. The intraday and interday precisions were <15%, and accuracies ranged from 80.3% to 111.0%. This validated method with high sensitivity has been applied in three anticoagulant rodenticide poisoning cases and has been used successfully in monitoring blood concentrations for months.     

Validation of Reduced Reagent Volumes in the Implementation of the Quantifiler® Trio Quantification Kit

The Quantifiler^® Trio Quantification Kit has been developed to quantify the total amount of amplifiable and human male DNA in samples and to estimate the extent of DNA degradation. To minimize the cost of DNA quantification, we evaluated kit performance using a reduced volume of reagents (1/10‐volume) using DNA samples of varying types and concentrations. Our results demonstrated concordance between the manufacturer's method and the low‐volume method for DNA quantification, DNA degradation index estimation, and human male DNA quantification. We confirmed the practical utility of the low‐volume method with 109 casework samples by evaluating short tandem repeat (STR) profiling success with respect to DNA quantity and quality. We also defined a cutoff value for DNA quantity to ensure reliable STR results. Using a reduced volume of reagents, 10 times more reactions per kit are possible; accordingly, this method reduces the cost of DNA quantification, while maintaining performance.     

从苏共教训看执政能力建设的重要

上世纪90年代初,世界上第一个社 会主义国家苏联,在一夜之间国家解体, 政权垮台。苏共,这个具有88年历史的大 党,在它拥有20万党员的时候,领导人民 夺取政权,赢得俄国十月革命的胜利,同 时在被帝国主义的重重包围下,迅速发展 壮大;在它拥有200万党员的时候,领导 人民打败了德国法西斯,捍卫了苏维埃政 权;到1991年,在它已经发展到将近 2000万党员的时候,却以令人难以置信 的速度和方式丧失了政权。     

检察官客观义务

检察官的客观义务是大陆法系检察官定位的基本特征,也是检察官具有司法官属性的必然体现和要求。检察官的客观义务原则,越来越得到世界各国刑事诉讼和司法制度的确认,体现为一种发展趋势。从该原则的发展中,我们能够更清楚地把握检察权在现代法治中的发展走向。     

液相色谱-串联质谱法测定血液和尿液中秋水仙碱

目的建立检测血液和尿液中秋水仙碱的液相色谱-串联质谱法。方法0.5mL血液或尿液以丁丙诺啡为内标,经pH9.2硼酸盐缓冲溶液碱化后,用乙酸乙酯进行提取,在ZORBAX SB-C18液相柱(150mm×2.1mm×5μm)上以V(甲醇)∶V(20mmol/L乙酸铵和0.1%甲酸缓冲溶液)=80∶20为流动相,流速为0.2mL/min,采用电喷雾正离子模式离子化、多反应监测模式检测秋水仙碱,内标法定量。结果血液、尿液中秋水仙碱与内标丁丙诺啡色谱分离良好,秋水仙碱在0.1~50 ng/mL内均具有良好的线性,相关系数>0.9990,最低检出限为0.05ng/mL,方法回收率为94%~116%,日内与日间精密度(RSD)均小于8.5%。结论所建LC-MS-MS方法灵敏度高、操作简便、快速、准确,适用于血液及尿液等生物检材中痕量秋水仙碱成分的检测。