讯问方法探析

侦查讯问是公安机关调查取证的一个重要过程和专门业务活动,它也是刑事侦查的重要手段。本文通过分析不同犯罪对象的心理特征和不同的讯问方法,概括出实践性强的几种讯问方法的特点,从而提高侦查活动的效率。     

万山特区新农村建设面临的问题及对策

贫困落后地区把握好历史机遇克服困难,奋力推进社会主义新农村建设的根本出路在于争取国家政策倾斜,加强农村教育,提高农民素质;改善优化环境;加强农村基层组织建设,完善村级管理。     

讯问方法探析

侦查讯问是公安机关调查取证的一个重要过程和专门业务活动,它也是刑事侦查的重要手段。本文通过分析不同犯罪对象的心理特征和不同的讯问方法,概括出实践性强的几种讯问方法的特点,从而提高侦查活动的效率。     

建国以来中国共产党文化发展战略的演进与创新

建国以来,中国共产党文化发展战略的演进经历了战略奠基、战略布局、战略拓展、战略明晰四个阶段。在文化发展战略60年演进中,中国共产党对文化发展的规律性认识越来越深刻,对文化发展战略的诉求越来越自觉,对文化发展战略的思考越来越具有全球视野,对文化发展战略体系主要内容的认识越来越清晰。党的十七大以来,我国文化发展战略以提高国家文化软实力为新指向,这是中国共产党对新时期推动文化大发展大繁荣的创新成果。     

中国当今社会矛盾的特点与解决途径

自20世纪90年代以来,也就是近一二十年,中国处于社会矛盾的高发期,也是一些非对抗性矛盾演化为对抗性矛盾的突发期.有一个时期,犯罪率很高,大案、要案居高不下,青少年犯罪十年翻番.     

论欧盟法中跨国企业集团破产的管辖权

跨国企业集团是由在各国成立的不同法律实体以某种形式的控制权或所有权相互连结而形成的经济组织。在欧盟破产法中,跨国企业集团破产管辖权可以分成两个层次:一是基于债务人主要利益中心地的管辖;二是基于债务人营业所所在地的管辖。     

微小隐孢子虫和安氏隐孢子虫SYBR Green real time PCR检测方法的建立

根据GenBank中登录的隐孢子虫小亚基rRNA基因序列设计1对引物,并以标准基因组DNA为模板,初步建立了检测乳牛微小隐孢子虫和安氏隐孢子虫的SYBR Green real time PCR方法,并对乳牛微小隐孢子虫和安氏隐孢子虫阳性样品和上海40份乳牛粪便进行了检测.结果表明,此次建立的real timePCR对微小隐孢子虫和安氏隐孢子虫均能扩增出曲线,且其他寄生虫(鸡贝氏隐孢子虫、刚地弓形虫、犬新孢子虫)和大肠杆菌均未检测到;标准基因组DNA的检测阈值达到5个拷贝,牛粪中卵囊的最低检测量为每克粪便5个卵囊,乳牛粪便阳性率为15%(6/40).表明,建立的荧光定量PCR快速、特异、敏感,可用于乳牛隐孢子虫病的流行病学调查.     

Sudden Unexpected Death Due to Left Subclavian Artery‐esophageal Fistula Caused by Fish Bone

A 53‐year‐old woman was admitted to the hospital due to unexpected dizziness and died the following morning. To investigate the cause of death, a forensic autopsy along with histological examination was performed 3 days after her death. The major findings of the autopsy were that a fish bone had pierced the left subclavian artery after perforating the esophagus with 680 mL of blood in the stomach and bloody and tarry contents were present in the intestines, and the cause of death was confirmed to be subsequent hemorrhagic shock. Unfortunately, none of her family realized that she had eaten a fish 4 days before the tragedy until the fish bone was found. The present case is rare and instructive. The histopathological findings of left subclavian artery‐esophageal fistula induced by a fish bone can be used as a reference in forensic practice.     

Application of MALDI‐TOF MS for Estimating the Postmortem Interval in Rat Muscle Samples

Estimating the postmortem interval (PMI) is very important in the forensic sciences. Although many approaches have been used for estimating the PMI, accurate PMI calculations are still difficult. In this study, four Sprague Dawley (SD) rats were sacrificed by suffocation, and muscle samples were collected by dissection at various time intervals (0, 48, 96, and 144 h) after death. All samples were probed using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) to obtain molecular images and data for principal component analysis (PCA). The results showed that the peaks at m/z 1511, 1543, 1564, 1586 clearly decreased in intensity from 0 to 144 h postmortem and that the time groups were separated from each other on the PCA score plot. The prediction model showed high recognition capability (95.93%) and cross‐validation (83.72%). Our work suggests that MALDI‐TOF MS can be used to determine the PMI.     

应用膝关节MRI T_2加权脂肪抑制像推断活体年龄

目的探索膝关节MRI T2加权脂肪抑制像(T2-FS)在法医年龄推断中的应用价值,建立安全可靠的四川汉族年龄推断方法。方法收集膝关节MRI 324例(男性170例,女性154例),年龄范围为10~30岁。采用西门子1.5T磁共振仪摄片,MRI参数:T2-FS TSE序列,矢状位。将股骨远端、胫骨近端骨骺发育分为6个等级并赋分,分析各指标性别差异及年龄相关性,建立年龄推断回归模型。结果男女性股骨远端、胫骨近端MRI等级与年龄呈正相关性,股骨远端与年龄的相关性男性0.687,女性0.661;胫骨近端与年龄的相关性男性0.684,女性0.488。股骨远端骨骺完全闭合,骺线消失的最小年龄男性18.42岁,女性19.36岁;胫骨近端男性骺线消失的最小年龄为16.93岁,女性为14.68岁。股骨远端等级1~3、5、6和胫骨近端等级1~3、5年龄分布性别差异不显著。分别建立男女年龄推断模型,男性年龄推断平均绝对误差(MAD)=2.90岁;女性年龄推断MAD=3.30岁。结论膝关节MRI T2-FS影像可作为活体年龄推断的指标之一,股骨远端等级6可作为判断18岁的指标之一。