HLA基因分型技术的进展与展望

研究人的主要组织相容性复合物HLA(humanleucocyteantigen,人类白细胞抗原)的结构和功能有助于认识生命现象的本质以及疾病的致病机理,因此长期以来一直是生命科学研究的重点之一。随着分子生物学技术的迅速发展,HLA分型已经由传统的血清学水平向DNA水平过渡,并取得了很好的效果。本文对目前各种HLA基因分型方法的原理和优缺点结合自己的应用经验做了评价,并简要介绍了在HLA基因分型研究中出现的新的动态。     

家兔急性胰腺炎死后不同时间眼玻璃体液淀粉酶变化

目的探讨急性胰腺炎死后不同时间眼玻璃体液淀粉酶变化。方法用1%牛胆酸钠诱导家兔急性胰腺炎动物模型建立,于死后不同时间和正常对照组,观察其淀粉酶变化。结果家兔急性胰腺炎死后72h眼玻璃体液淀粉酶含量(x)与死亡时间(y)存在相关关系,并导出其二项式回归方程为y=8.7420 0.7699x-0.0083x2(R2=92.62792,F=14.89734,P=0.001)。可作为推定早期损伤时间的参考指标。结论家兔急性胰腺炎死后不同时间眼玻璃体液淀粉酶改变为法医早期死亡时间推断提供了灵敏客观的实验依据。     

Forensic identification of dyes extracted from textile fibers by liquid chromatography mass spectrometry (LC-MS)

LC-MS is used for the identification of dyes extracted from textile fibers and the utility of the method for forensic trace analysis is demonstrated. The technique is shown to provide a high degree of chemical structural information, making dye identification highly specific in comparison to optical and/or chromatographic methods of dye analysis. A UV-visible absorbance detector, placed in series before the MS detector, facilitates monitoring the elution of dyes in the presence of other non-dye components extracted from colored textile fibers. In this way, dye identification becomes practical, even when a dye standard is not available for comparison. A set of 22 reference dyestuffs and 10 dyes extracted from textile fibers were analyzed to demonstrate the utility of the method. Six of the extracted dyes corresponded to dyes also contained in the set of 22 reference dyestuffs. Reference dyestuffs were not available for four of the extracted dyes. Triethylamine (TEA) was shown to increase the electrospray ionization-mass spectrometry (ESI-MS) response of dyes containing multiple sulfonated groups.     

Characterization and haplotype analysis of 10 novel Y-STR loci in Chinese Han population

In this study, we analyzed allelic sequences of 10 novel Y-specific STR loci, DYS454, DYS510, DYS513, DYS520, DYS542, DYS544, DYS552, DYS561, DYS587 and DYS593, surveyed the distribution of haplotypes in a Chinese Han population. Extracted DNA was amplified with PCR, followed by a horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system. Purified alleles were sequenced on DNA sequencer (ABI Model 377) to verify the number of motif repeats. The number of alleles observed at each locus ranged from 3 to 8, yielding 102 haplotypes in 103 unrelated males samples. The allele diversity values for each locus ranged from 0.2099 (DYS544) to 0.7523 (DYS552). The haplotype diversity using all these loci was 0.9998. Our study revealed that they were valuable Y-specific markers for forensic applications.     

论邓小平的小康社会思想

邓小平对小康水平、小康状态、小康生活、小康之家、小康社会、小康的中国等概念的论述,蕴含着中国特色社会主义经济、政治、文化全面发展的思想,为全面建设小康社会和实现社会主义现代化奠定了坚实的理论基础。党的十六大提出的"全面建设小康社会"的奋斗目标和行动纲领,是对邓小平小康社会思想的丰富和发展。     

“网络援助”系统工程的可行性探讨

首次提出了"网络援助"概念.网络援助系统的出现,可以以第三方介入的形式来帮助计算机应用人员缓解信息技术快速发展带来的压力,提高计算机应用人员的应用能力,从而缩小"技术鸿沟",秉持"普遍性服务"和"公平近用"的理念,挖掘信息技术的应用和发展潜力,以人为本,推动信息技术的普及.