从“血荒”现象看《献血法》在实施中的问题与完善

我国《献血法》在实施中存在一些问题：“血荒”现象在很多地方存在;目前的还血政策不能很好地激励公民献血的积极性;法律对医疗机构紧急用血规定不完善,“见死不救”,“救人违法”的矛盾在现实中还存在;部分临床用血的血液来源存在安全隐患。应当教育、动员、招募一支固定无偿献血者队伍,适当放宽献血的条件。提高血站的管理水平和服务质量、加强无偿献血及免费用血系统的网络化建设、适当扩大医疗机构在紧急用血时的权限。     

少数民族地区产业园区土地开发的企业化模式设计——以贵阳金石产业园为例

企业化经营是未来产业园区的发展趋势,园区土地的开发主体也逐渐由政府转为企业。在回顾现有国内现有产业园区土地开发模式研究的基础上,以贵州省贵阳市金石产业园为个案探讨,从而为西部产业园区土地开发模式的选择提供理论与实践支持,也为实现失地农民特别是少数民族聚居地的可持续发展提供新的思路。     

Comparison of feature selection and data fusion of Fourier transform infrared and Raman spectroscopy for identifying watercolor ink

The identification of different kinds of watercolor inks is an important work in the field of forensic science. Four different kinds of watercolor ink Spectroscopy data fusion strategies (Fourier Transform Infrared spectroscopy and Raman spectroscopy) combined with a non-linear classification model (Extreme Learning Machine) were used to identify the brand of watercolor inks. The study chose Competitive Adaptive Reweighted Sampling (CARS), Random Frog (RF), Variable Combination Population Analysis-Genetic Algorithm (VCPA-GA), and Variable Combination Population Analysis-Iteratively Retains Informative Variables (VCPA-IRIV) to extract characteristic variables for mid-level data fusion. The Cuckoo Search (CS) algorithm is used to optimize the extreme learning machine classification model. The results showed that the classification capacity of the mid-level fusion spectra model was more satisfactory than that of single Infrared spectroscopy or Raman spectroscopy. The CS-ELM models based on infrared spectroscopy used to recognize the watercolor ink according to brands (ZHENCAI, DELI, CHENGUANG, and STAEDTLER) obtained an accuracy of 66.67% in the test set using all spectral datasets. The accuracy of CS-ELM models based on Raman spectroscopy was 67.39%. The characteristic wavelength selection algorithms effectively improved the accuracy of the CS-ELM models. The classification accuracy of the mid-level spectroscopy fusion model combined with the VCPA-IRIV algorithm was 100%. The data fusion method increased effectively spectral information. The method could satisfactorily identify different brands of watercolor inks and support the preservation of artifacts, paintings, and forensic document examination.     

弓形虫烯醇化酶2基因的原核表达与鉴定

为深入研究弓形虫烯醇化酶的生物学功能,根据已发表的eno2基因序列设计引物,通过RTPCR方法扩增弓形虫RH株的eno2基因。将eno2克隆到载体pET-28a(+)中后,重组质粒转化大肠杆菌BL21(DE3)感受态细胞,IPTG诱导表达并优化表达条件,SDS-PAGE分析表达情况,并通过Western-blot和间接免疫荧光试验(IFA)鉴定其免疫活性。结果显示,eno2基因全长1 353bp,编码444个氨基酸,与GenBank中登录的弓形虫烯醇化酶基因的核苷酸序列及其推导的氨基酸序列的同源性均高达99.8%,与其他物种的烯醇化酶的同源性较低。在pH7.5、28℃和IPTG终浓度0.1mmol/L条件下,重组蛋白ENO2得到高效可溶性表达,分子质量约为55ku。Western-blot结果显示,重组蛋白能被感染弓形虫的犬阳性血清识别,说明ENO2具有较好的反应原性,可以作为血清学诊断抗原。IFA表明,NO2在弓形虫的细胞质和细胞核中均有分布。结果表明,本研究成功获得了可溶性、具有良好免疫活性的弓形虫重组蛋白ENO2,为深入研究其生物学功能奠定了基础。     

急性乙醇中毒和外伤在蛛网膜下腔出血致死机制中的作用

目的研究人脑在急性乙醇中毒状态下tPA、MMP-2、MMP-9及AEG-1表达水平与乙醇浓度的关系,分析乙醇与外伤在蛛网膜下腔出血死亡机制中的作用。方法选取15例实际案例,提取脑组织进行组织病理学检查、心血进行乙醇浓度检测,用免疫组织化学法观察人脑干、大脑、小脑tPA、MMP-2、MMP-9及AEG-1表达水平。结果急性乙醇中毒外伤组和急性乙醇中毒组的脑组织肿胀。除小脑tPA外,tPA、MMP-2、MMP-9及AEG-1表达水平在饮酒者较未饮酒者在脑干、大脑、小脑均有升高(P0.05),MMP-2、MMP-9和AEG-1表达水平与心血乙醇浓度存在正相关(P0.05,r0.6)。结论急性乙醇中毒状态下人脑tPA、MMP-2、MMP-9、AEG-1表达较未饮酒者均显著增强,MMP-2、MMP-9及AEG-1表达水平与乙醇浓度存在相关性,乙醇对脑细胞有毒性作用。     

论我国《物权法》上的商事留置权

我国《物权法》第231条规定了商事留置权制度,其成立要件在权利义务主体、债权的发生和动产的占有等方面与民事留置权相比有所不同。商事留置权可以适用善意取得制度。在去法典化的时代,我国采用民商合一的立法例有其合理性,商事留置权制度较好地协调了民商合一的立法例与商法的特殊需求,其立法理念和立法技术值得发扬。     

Fiscal slack or environmental pressures: which matters more for technological innovation assimilation? A configurational approach

Abstract

Prior studies suggest that fiscal slack may positively affect technological innovation in the public sector but yield inconsistency between theoretical expectations and empirical findings. To address this research gap, this study develops a configurational explanation combining fiscal slack and environmental pressures by employing fs-QCA in the context of Chinese provincial governments’ online public service provision. The findings reveal that the role of fiscal slack is environmentally dependent on explaining technological innovation and that environmental pressures seem to be more important. That is, fiscal slack is likely irrelevant to a high degree of technological innovation assimilation, while the demanding environments of multiple environmental pressures matter. Relative fiscal scarcity may be somewhat relevant to a low degree of technological innovation assimilation only when combined with a more relaxed environment. Several theoretical and practical implications are discussed.     

ICF脑卒中核心分类组合功能的图形建模研究

目的依据《国际功能、残疾和健康分类(ICF)》脑卒中核心组合,探讨脑卒中相关功能类目之间的关系,绘制ICF脑卒中核心分类组合的功能地形图,为脑卒中的伤残评定提供科学理论依据。方法收集106例脑卒中被鉴定人样本,采用"最小的绝对缩减和变量选择算子",研究脑卒中ICF核心组合功能的59项类目变量之间的条件依存关系,并用图形建模分析类目之间的内在联系,绘制功能地形图。结果 59项类目之间的相互联系构成了功能地形图,b340、b735、b175和b152因关联性较高而居于地形图的中心位置。结论脑卒中功能地形图可以揭示ICF功能类目之间复杂的关系结构及关联程度。可以为应用ICF进行脑卒中的伤残评定提供科学理论依据。     

基于路径依赖的制造业产业升级研究——以广东东莞为例

利用先发优势,凭借比较优势,东莞制造业发展迅速。产业规模扩大带来的报酬递增,使越来越多的企业从事劳动密集型产品加工生产,形成了东莞制造业大批产业集群的现象,东莞已成为全球著名的国际性加工制造业基地,东莞制造业的出口加工型"路径依赖"特征十分明显。进入新世纪后,先发优势削弱、成本优势递减、国际竞争压力加大,使东莞制造业面临着产业升级的压力。本文试图对东莞制造业如何走出传统路径依赖,实现结构优化升级作些研究。     

侵犯知识产权犯罪若干问题研究

本文以研究知识产权刑法保护的内容及意义为基本切入点,主要阐述了世界主要国家知识产权刑法保护的内容和范围以及我国刑法保护知识产权的立法内容和范围。并根据全球化背景下知识产权犯罪态势变迁的现状,总结归纳了目前知识产权刑法保护所面临的挑战。