猪链球菌2型多重PCR快速检测方法的建立

根据猪链球菌16 S rDNA基因的种特异性基因序列和CPS基因的2型(1或/和2型)型特异基因序列设计了4条引物,建立了快速检测猪链球菌2型(1或/和2型)的多重PCR方法。利用保存的猪链球菌2型菌株和其他相关标准菌株作为参考菌株对该方法进行了敏感性和特异性试验。结果显示,所建立的多重PCR方法特异、敏感,对组织中的猪链球菌2型(1或/和2型)的最低检出水平为30 CFU/mL。用所建立的多重PCR方法对采自江西、北京、珠海、天津、四川等省市的猪扁桃体样品进行了检测,并通过细菌分离和玻片凝集试验对其检测结果进行了验证,结果显示,检出阳性符合率为100%,证实建立的多重PCR方法可用于猪链球菌2型的快速检测。     

牛分枝杆菌重组MPT83蛋白间接ELISA方法的建立

以纯化的牛分枝杆菌重组MPT83蛋白为包被抗原,建立了检测牛分枝杆菌抗体的间接ELISA方法。确定了间接ELISA各组分的最适反应条件:抗原包被浓度为1μg/mL,酶标二抗稀释度为1∶1600,血清稀释度为1∶60,抗原和血清、血清和二抗均在37℃反应30 min,底物在37℃显色15 min,D655 nm阴性、阳性临界值为0.5。经阻断试验、交叉试验、重复性试验,表明该方法特异性强、重复性好。用该方法对18份结核菌素试验阳性牛血清和36份结核菌素试验阴性牛血清进行检测,结果显示,阳性血清的符合率为27.8%,阴性血清的符合率为91.7%。     

农村公共文化民生建设的理论原则与实践途径——以吉林省为例

保障和改善文化民生不仅是满足人民群众精神文化生活需求的现实任务,也是维护和保障公民文化基本权利实现的具体体现。这对政府健全公共文化服务体系提出了更高的要求。为满足这一要求,政府在改进提供公共服务方式,加强公共文化民生建设过程中要遵循公平适度、需求导向、社会协作的三条原则。在公共文化民生建设过程中,要推进队伍建设,为文化发展储备优秀人才;推进制度建设,为文化发展提供制度保证;推进社会建设,为文化发展提供发展载体。     

论新农村公共服务供给方式的选择

在市场因素作用不断增强、农民对公共服务需求量日益增长和需求日趋多样化的背景下,我国的农村公共服务供给方式应进行市场化改革。由于政府供给和市场供给方式在效率方面各有优缺点,而且不同地区农村居民对公共服务的需求存在差异,应根据公共服务的类别和性质选择适合的供给方式,才能更好地满足新农村建设时期农民对公共服务的需求。     

Taiwan’s investment policy in mainland China a domestic perspective

The interaction of politics and economics has complicated Taiwan’s investment in mainland China. Do we need to put some pressure on Taiwan’s investors and ask them to follow the “Jie Je Yong Ren” policy? Or can we just let them go, according to the principle of market liberalization? It is indeed very difficult to find an absolute solution. Again, it is also not easy to identify which perspective, state or market, is right or wrong. Although the conflictual situation between the government and the entrepreneurs will continue in the future, at least some of the myths relevant to this political and economic interaction across the Strait must be cleared up. Hopefully, this paper might provide some implications for both political decision-makers and enterpreneurs in Taiwan.     

Discrimination of phenethylamine regioisomers and structural analogues by Raman spectroscopy

In this study, the Raman spectra of 21 phenethylamines were obtained using far‐red excitation (785 nm). The distinguishing ability of Raman for phenethylamines, especially for phenethylamine regioisomers and structural analogues, was investigated. Here, the evaluation of a cross section of Raman spectra demonstrated that all types of phenethylamines were distinguishable, even for certain structural analogues with high spectrum similarity. Raman exhibited high distinguishing ability for phenethylamine regioisomers that differ in the substitution position of halogen, methoxy, alkyl, or other substituted groups; as well as for structural analogues containing different groups, such as furanyl, 2,3‐dihydrofuranyl, halogen, and alkyl substituted at the same position. The Raman spectra for homologues with differences in only a methyl group were found to be highly similar; however, their spectra demonstrated small but detectable differences. Four analogue mixtures and 59 seized samples were also analyzed to study the practical use of the Raman method in forensic field. 95% of the seized samples were correctly identified, which significantly validated the ability of Raman method in identifying the correct isomers. Accordingly, this study provides a non‐destructive, high‐throughput and minimal sample preparation technique for the discrimination of phenethylamines.     

论国际投资争议的“强制性仲裁”

传统理论与实践都认为，自愿性是仲裁的基础和核心。但随着国际社会与国际交往的发展。在国际投资领域已经开始出现了强制性仲裁。国际投资领域中的强制性仲裁的出现，对于国际商事仲裁的发展具有一定的影响，是国际商事仲裁发展的新动向，而且随着发达国家通过投资条约及其他国际条约日益加强对投资者的保护。以及发展中国家日益加强对外国投资的吸引力度，强制性仲裁可能会有更大的发展。其所涉及范围也可能从投资领域扩及到其他领域。     

论我国国家所有权立法及其模式选择——一种法和经济学分析的思路

根据我国物权立法和产权改革的实践 ,以及法和经济学的理论和方法 ,国家所有权在主体、客体、行使和立法模式选择几个方面都有其特性 ,在我国市场经济条件下的物权法及其相关所有权立法中存在着重要价值、地位和作用     

论新时期的理想信念教育

理想信念教育应和灌输科学的思想理论,三讲教育,爱国主义教育,世界观、人生观、价值观教育,坚持学习与实践,拓宽教育渠道,更新教育方法等各个方面结合起来,使新时期的理想教育更有实效.