知识产权案件代理难点问题之我见

知识产权案件具有特殊性.在代理知识产权案件时,除了与一般的民事案件要经历相同的程序外,还有一些难点问题需要特别注意.知识产权案件专业性都比较强,接受委托要慎重;代理原告一方时作好起诉前的准备工作一定程度上解决了起诉方面临的难点问题;代理被告一方时作好应诉的准备解决了应诉的难题;知识产权案件庭审时紧围焦点,从容应对解决了开庭审理时的难点问题.     

A Y‐Chromosomal Haplotype with Two Short Tandem Repeat Mutations*

Abstract:  The male‐specific Y‐chromosomal short tandem repeat (STR) is a useful tool in forensic casework. The Y haplotype comprised of 16 loci, which is amplified simultaneously by AmpFlSTR^® Yfiler^TM PCR kit and provides strong exculpatory evidence in individual identification. We reported a rare Y‐STR profile with a null allele at the DYS448 locus and an off‐ladder allele at the DYS456 locus, when genotyping material from a vaginal swab in an alleged rape case. Sequence analysis revealed that the DYS448 null allele was a true type of null allele because of a total deletion of 11 upstream repeats and 9 bp of the N₄₂ region, and there were numerous primer binding site mutations as well. The amplicon of the DYS456 locus was a small 92‐bp fragment that was off‐ladder, and sequencing analysis showed that there were only 10 repeats (AGAT)₁₀. This Y chromosome haplotype that was comprised of two variations provided helpful evidence for personal identification.     

Determination of bromadiolone and brodifacoum in human blood using LC-ESI/MS/MS and its application in four superwarfarin poisoning cases

Superwarfarin poisoning is a growing health problem. A sensitive and reproducible LC-ESI/MS/MS (liquid chromatography electrospray ionization tandem mass spectrometry) method was developed and validated for the determination of bromadiolone and brodifacoum, the most commonly used superwarfarins, in human blood using warfarin-D5 as an internal standard. Bromadiolone and brodifacoum were extracted from whole blood samples by liquid-liquid extraction with ethyl acetate. Multiple-reaction monitoring (MRM) was used to detect bromadiolone and brodifacoum using precursor→product ion combinations of m/z 525→250 and 521→135, respectively. The calibration curves were linear (r(2)=0.9999) in the concentration range of 0.5-100.0ng/mL for bromadiolone and brodifacoum, with a lower limit of detection of 0.1 and 0.2ng/mL, respectively, in whole blood. This method detected trace levels of bromadiolone and brodifacoum in whole blood samples and can be used in the diagnosis of poisoned human beings.     

免疫磁珠技术和精子抗原研究进展与法医学应用

免疫磁珠技术在生物学领域应用广泛,通过制备精子特异性抗体,并与磁性微球偶联,可达到对精阴混合斑中精子和女性阴道上皮细胞分离。本文简述了免疫磁珠技术的研究进展及磁珠主要制备方法,同时结合精子表面抗原的研究进展,提出磁珠抗体法提取精子DNA的可能性,并展望了磁珠抗体结合技术在法医学应用前景。     

印记基因5个SNP分型及亲代来源的检测

目的 采用复合PCR-Snapshot联合甲基化敏感酶切技术,检测印记基因中5个SNP的甲基化状态、印记亲代来源及分型.方法 选择15例亲子鉴定已证实为亲生关系的家系样本,采用单碱基延伸复合检测技术,检测家系样本IGF2AS rs1003483、SNURF rs220028、SNURF rs4906939、DLGAP2 rs6558478、SIM2 rs737380等5个SNP分型,同时选用核酸内切酶(McrBC)和甲基化敏感的限制酶(msRE) HhaⅠ、HpaⅡ消化子代DNA,验证印记基因的亲代来源.结果 经用本文方法检测,证实rs1003483为父源印记;rs220028、rs4906939为母源印记;rs6558478及rs737380未在差异甲基化区,不能确定其印记亲代来源.结论 复合PCR-Snapshot联合甲基化敏感酶切技术简单、高效,在检测多个SNP分型的同时可确定亲代来源,可在相关研究和实践中选用.     

研究生教育转型发展背景下法律硕士教育改革思考

自2008年国家开始稳步推进研究生教育转型发展工作以来,我国专业学位研究生教育得到了跨越式发展。法律硕士研究生教育作为专业学位教育的重要组成部分,在国家宏观教育改革的大背景下,发展也十分迅速:逐步建立和完善了分层次、分类别培养的教育体系,培养规模增长迅速,教育试点改革工作顺利开展。但在高速发展的背后,人才培养质量却并不理想。研究生教育转型发展和专业学位发展不仅仅是培养规模的扩大,更重要的是全面提升人才培养质量,形成职业化导向的鲜明教育特色,并最终使之成为我国法律职业人才培养的主渠道。     

A Migrant’s Tale

正Documentary peeks into the lives and struggles of China’s migrant workers F or most of the 130 million Chinese migrants who make the annual pilgrimage from the country’s urban centers to their rural hometowns or interior cities for China’s Spring Festival holiday,the stories of their arduous journey home go