Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

In this report, we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle, and to provide information for follow-up studies on neutrophils to estimate wound age. Rat injury model was established, and skeletal muscle samples were obtained from the control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as at 1, 3, 5, and 15 d post-injury (n = 5 per group). The expression of nuclei and neutrophils was detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. A total of 20 injury site areas of 0.25 mm² (0.5 mm × 0.5 mm) were then randomly selected at all time points. A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative numbers. Knowledge of five professional medical workers were considered the gold standard to measure the false positive rate (FPR), false negative rate (FNR), sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) curves. As a result, with a staining area of neutrophils from 8 µm² to 15 µm², the FPR was 4.28%–12.14%, the FNR was 12.42%–64.08%, the sensitivity was 35.92%–87.58%, the specificity was 87.86%–95.72%, the Youden index was 0.316–0.754, the accuracy was 82.80%–88.30%, and the AUC was 0.771–0.826. The AUC was largest when the cut-off value of the staining area was 12 µm². Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil infiltration. Based on the sensitivity and specificity, neutrophils can be accurately identified during regeneration of contused skeletal muscle. The TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further study. Furthermore, it will provide evidence for forensic pathology cases.     

Comingin From the Cold

<正>Having fought each other to a standstill over the Ukrainian crisis,the United States and Russia seek a path to peaceful coexistence On June 25,Russian President Vladimir Putin gave U.S.President Barack Obama a telephone call.This was the first direct talk between the two heads of state since February,and was described by NBC News as     

朝鲜族乡村社会的集中村建设及其社会共同体的质变

城镇化进程将导致传统村落共同体的解体与巨大社会变迁.引导乡村社区的整合与重构,摸索乡村社会可持续发展模式是必要的.朝鲜族乡村社会在城市化进程中经历了巨大社会变迁.为了克服社会变迁带来的阵痛,朝鲜族社会自发组织起集中村建设运动,通过集中村的形式实现就地城镇化的道路.尊重经济结构形式与社会共同体形式之间关系的规律,成为城镇化背景下乡村社区重构的关键因素.集中村建设经验对于处于社会转型期的中国乡村社会,具有启示性的意义.     

金融犯罪刑事司法政策评述

随着金融市场的发展,犯罪防控的形势日益严峻,金融犯罪刑事司法政策一直致力于回应金融市场当前面临的现实发展状态及问题。但是政策效果受到立法制约和影响,且与金融监管联系紧密。为了寻求金融犯罪刑事司法政策的完善路径,首先需要理解金融犯罪司法规制的能力范围,了解金融的特性,理解刑法的局限。在此基础上,有针对性地落实宽严相济刑事政策,并且发挥金融犯罪刑事司法政策的独立价值。     

19世纪中晚期朴学的延续与流变

19世纪中后期,即在道成以来,朴学仍具有相当的活力,甚至同光年间,其流风余韵一直未断,取得了不亚于乾嘉时期的学术成就.考据与义理会通、考据与经世贯通成为此期朴学流变的显著特征.     

保护、惩治与预防——我国少年司法制度变迁七十年(1949—2019)

新中国成立以来,以保护青少年权益为核心目的的少年司法制度经历了从无到有、从初步架构到日趋完善的阶段。其中,以《未成年人保护法》《预防未成年人犯罪法》为代表的专门立法文件,确立了我国教育为主、惩治为辅的少年司法原则,同时设置了具体的未成年人特别司法程序和审判要求,以契合少年司法制度的整体价值取向。少年法庭、少年检察机构的相继设立与发展,为实现上述司法干预措施提供了组织保障。但现有的依托于刑事案件的单一处理模式具有一定的局限性,未来应当在区分式的独立少年法体系中,将多元的保护处分、分级处遇程序和关注于事先预防的临界预防程序纳入现有的司法干预体系中,以教代刑,以更好地实现对青少年不良行为及触法行为的矫治和预防。     

“红船精神”之时代价值论析

红船精神所蕴含的首创精神、奋斗精神和奉献精神,已经形成了一种永恒的文化品格。在中国革命、建设与改革的不同阶段,红船精神具有不同的价值。新时代,红船精神的价值体现在四个方面:红船精神是激发创新精神的思想源泉,是坚定理想信念的鲜明旗帜,是增强文化自信的精神力量,是激励奋进不息的生活态度的现实动力。     

网络意识形态安全面临的挑战和应对措施研究

在互联网+时代,网络意识形态安全在维护政权稳定、确保国家安全和凝聚中华民族伟大力量、认可社会主义意识形态中都发挥着非常重要的作用。伴随着互联网的迅猛发展,网络意识形态安全面临前所未有的挑战。切实做好维护网络意识形态安全工作,引导网络主流意识形态,是党和国家面临的新课题。     

试论我国洗钱犯罪侦查工作中的难点及对策

洗钱犯罪是我国新时期面临的重大犯罪威胁之一,其行为所具有的隐蔽性、复杂性、专业性,以及与上游犯罪紧密联系的特征等使该类犯罪的侦查工作面临严峻的困难和挑战。从侦查学角度,探讨我国洗钱犯罪侦查工作的对策,进一步完善反洗钱的立法工作,以保证我国反洗钱工作的顺利开展。