心脏传导系统的一种新型染色法

本文建立了一种研究心脏传导系统和心血管形态学的特殊染色技术（改良的Ｍａｓｓｏｎ’ｓ三色法十Ｖｅｒｈｏｅｆｆ’ｓ弹力染色法），能在同一玻片上将起搏细胞、工作心肌、胶原纤维及弹力纤维区分开来，且操作简捷，色彩对比鲜明。     

Views of Male University Students About Social Gender Roles; An Example From East of Turkey

Social gender roles refer to the roles that are traditionally associated with women and men. Social gender role includes the personal attributes and behaviors which are culturally assigned to women and men. This study was conducted to determine the views of male students at Caucasian University Health School on social gender roles at work, social life, marriage, and family life. The study was conducted on male students studying at Kars Health School, Nursing and Health Officers Department during 2007–2008 academic year. The students were given 24 statements relating to work life, social life, marriage, and family life to determine their views on social gender roles. Results indicated that 30.2% of the male students stated that women could work in a paid job, 56.9% believed in equality of women and men, 44.8% approved honor killing, 40.5% said the girls should receive education as far as they can go, 54.3% said the role of the women was to “provide moral support to their husband and children”, 37.1% stated that husbands could beat their wives under certain circumstances, 52.6% said they witnessed violence in their family at some stage of their lives, 51.7% said the women’s environment should be decided by the spouses together, 25% said the women should engage in sexual intercourse with their spouses even if they did not want to. Men who thought the role of the women was to do housework/giving birth to children/looking after the elderly members of the house, and who approved honor killing and disapproved working of their spouses, and who did not believe in equality of women and men, were found to support violence to women by men. Moreover, the students who witnessed violence at some stage of their lives supported this view as well. It was considered that the students should be educated on the definition of violence and situations involving violence, and directed to consultant services.     

应用RNA分析技术推断现场血痕形成时间

目的检测血痕中β-actin mRNA和18S rRNA在人体死后8～15d期间的表达残留,为推断血痕形成时间寻找新的客观依据。方法在上述时段内每天抽提血痕总RNA,利用实时定量RT-PCR技术监测18S rRNA和β-actin mRNA的扩增状况并对产物进行定量分析,通过检测18S rRNA与β-actin mRNA含量在各个时间点的变化趋势来推测血痕形成时间。结果死后8～15 d时段内18S rRNA与β-actinmRNA量的比值呈明显升高趋势,显示出两种不同类型RNA降解的时间差异性。结论一定时段内18SrRNA与β-actin mRNA量的相对变化,可作为推测血痕形成时间的参考指标。     

SMCY性别特异融合抗原的克隆表达及其抗体制备

目的利用蛋白检测的快速性优势,研究不同性别在SMCY抗原氨基酸序列的差异,筛选出特异性氨基酸序列,并克隆表达性别特异融合抗原,制备相应抗体,建立一种快速鉴别法医物证性别的方法。方法通过对人SMCY和SMCX进行序列分析,发现了三段差异片段,采用搭桥PCR方法获得差异片段全长基因,连接入p ET-28a载体进行原核表达,用Ni柱纯化后的性别特异融合抗原免疫制备多克隆抗体,用ELISA法和western blot检测SMCY多抗与抗原的反应特异性,制作胶体金试纸条检测样本。结果筛选出具有性别特异性的氨基酸序列,获得SMCY性别特异融合抗原,成功制备出多克隆抗体及胶体金试纸条。结论获得SMCY性别特异融合抗原具有很好的抗原活性,制备的多克隆抗体可以与抗原特异性地结合,用于性别鉴定。     

福尔马林固定组织SNP与STR检测的比较

目的检测经长期福尔马林固定的组织降解情况,并比较组织中SNP与STR的检出率。方法本文对24例经福尔马林固定、-20℃保存5年的组织样本,采用Quantifiler?Trio DNA定量试剂盒检测样本DNA的降解系数及浓度,运用55-SNPs SNa Pshot复合分型体系和Power Plex?21试剂盒分别进行SNP与STR检测。结果大部分样本降解系数在1~8,发生不同程度的降解。与未降解样本相比,SNP分型完全一致,检出率为100%;其中8例样本STR分型存在33个等位基因丢失,降解系数均大于2.6,且75.8%的等位基因片段长度大于300bp。当样本检测出16个STR基因座时,似然率与54个SNP相当。当样本检出大于17个STR时,似然率大于54个SNP。STR基因座片段长度与等位基因检出率之间呈负相关。除2例样本降解系数较小却发生等位基因丢失外,其余样本降解系数与等位基因检出率之间呈负相关。结论经福尔马林长期固定的组织DNA易降解,检测SNP明显优于STR,但需要更多的SNP以提高个体识别能力。     

日本与东盟科技合作研究

日本既是当今世界第二大经济大国,也是一个科技强国。由于人口众多、国土狭小、资源匮乏,长期以来日本走的是一条面向全球发展经济的道路。加上日本地理上邻近东南亚,相互往来历史悠久,双方在社会、政治、经济和文化等领域保持着密切的关系,因而战后日本一直十分重视对东南亚地区的市场开拓。早在1977年,日本首相福田纠夫在首届日本—东盟首脑会议结束后,就提出了要与东盟发展“对等的伙伴关系”和“心心相印的信任关系”。以此为契机,日本在经济、社会和文化等众多领域与东盟进行合作并提供了大量的政府开发援助(ODA)①,并且随着其产业技…     

温州汉族人群D7S2846、D19S400和D18S535基因座的遗传多态性研究

目的研究D7S2846、D19S400和D18S535位点在温州汉族人群的遗传多态性。方法EDTA抗凝血样采自194名无血缘关系温州地区汉族个体,用chelex-100法提取DNA,PCR扩增,聚丙烯酰胺凝胶电泳,银染显色分析。结果D7S2846观察到6个等位基因及15种基因型;D19S400观察到10个等位基因及36种基因型;D18S535观察到8个等位基因及26种基因型。各基因座的杂合度(H)分别为:0.644、0.724、0.772;个人识别能力(Dp):0.854、0.940、0.938。结论三个STR基因座具有较高杂合度,等位基因分布符合Hardy-Weinberg平衡,在法医学应用和群体遗传学研究中有较高的价值。     

中国援越抗美支援部队出国40周年回眸

在越南战争期间,我国政府应越南政府的请求,先后派出32万多工程和防空部队到越南北方,并无偿提供了大量的武器装备和其他作战物资,协助越南军民抗击美国侵略。中国支援部队用生命和鲜血对越南人民的抗美战争作出重大贡献。     

诚实证人证言或然性探析

证人证言具有较强的证明力,具有直接证明力。诚实证人的证言,往往被采信的概率比较大。在司法实践中,由此而形成的错捕、错判、错杀的冤、错案不在少数,有的甚至可以说是触目惊心。司法办案人员必须科学地认识诚实证人证言,树立正确的取证观,客观、全面地收集和核查诚实证人证言,确保公正司法、严格执法,依法保障公民的合法权利。     

蒙古政坛的黑马--新任总理米·恩赫包勒德

2006年1月25日,蒙古国家大呼拉尔(议会)全体会议经投票表决,蒙古人民革命党(简称人革党)主席米·恩赫包勒德以83.3%的支持率获准出任蒙古国新政府总理.28日,恩赫包勒德提名的17名阁员中有16名得到了议会任命,新政府的组阁基本完成.至此,因蒙古联合政府解散而引发的政治风波暂告平息.