猪囊虫抗原基因TS21的真核表达

构建了猪囊虫抗原基因TS2 1的VR10 2 0真核表达载体 ,以菌落原位杂交法筛选获得正确插入的重组质粒 ,用RNA印迹 (Northernblotting)和ELISA检测插入片段的转录及其翻译表达产物。结果 ,VTS2 1在真核细胞中能够转录TS2 1基因的mRNA ;兔抗猪囊虫超免疫血清可识别其蛋白表达产物 ,表明VTS2 1能正确表达猪囊虫抗原蛋白     

论我国地方性公共物品的超地方化问题——以瑞安“就学阳光政策”的困境为例

我国区域经济发展的不平衡带来的人口流动增加,致使地方性公共物品在供给和需求两个方面出现超地方化问题。瑞安的“就学阳光政策”的困境就是这一问题的典型例证。解决地方公共物品的超地方化问题各级各地政府负有重要的责任和义务,寻求消解的对策应该考虑由中央统一供给,增加对公共物品的投入;改变公共物品的供给方式,寻求制度创新;加强地方间的合作,推进地区经济均衡发展;以及通过民营化方式增加公共物品供给等。     

努力营造良好的创新环境 不断提升公务员的创新能力

创新是一个国家不断发展,在国际竞争中取得主动地位的重要决定因素。南宁市在加快北部湾经济区开放开发的大好时机中,需要创造更好的投资环境,提供更多的公共服务。面对发展的机遇和困难,实现工作的新突破,各级政府及其公务员必须多途径地大力提升自身的创新能力。     

论心理测试方法在侦查中的选择和运用

国内外流行的三大心理测试方法是准绳问题测试法、紧张峰测试法和犯罪情景测试法。侦查阶段运用心理测试的方法要区分不同的案件情况对这三种测试方法作机动灵活的选择和运用 ,从而为侦查破案提供科学依据。     

我国物业管理立法问题探讨

近年来,我国物业管理迅速发展,尽管我国已有相关物业管理法律、法规、规章,但难以适应物业管理发展的需要。因此,我们弄清我国物业管理立法中存在的不足之处,探索完善立法问题,具有重要的现实意义。     

Economic Development and the Implementation of Village Elections in Rural China

Based on survey data from rural Fujian Province of China, this paper shows that economic development, especially village collective revenue, plays an important role in village elections. With economic development and industrialization, collective revenues will increase and differentiated interests will develop among villagers. Village elections are institutional channels for villagers to articulate their interests. The research findings show that it is the collective revenue and the relative living standard of the village compared with other townships within the same county or district that arouse villagers' interests for participation and candidates' motives for campaigning, and thus enhance the implementation of elections. The paper concludes that village-level economic development is crucial for the implementation of competitive elections. With economic development, village committees will control more collective revenues, thus increasing the stakes villagers have in elections. This will result in greater participation by villagers in elections, and in more competitive elections. Increased competitiveness of elections will facilitate changes in the institutions regarding village committee elections.     

WTO与中国税收立法的改革

1994年,我国进行了一次深刻的税收法制改革,从此以后一个新的税法体系开始运转,并在一段时期内对我国经济和社会的发展进程起到了积极的作用。然而,1994年税制改革仅仅只是朝着重构我国税法体系这个漫长的道路上迈出了最初的一步。为了与我国经济体制转型和政府财政行政管理方式变革齐步并进,适应我国加入WTO的需要,我国迫切需要进行新一轮的税法体系的改革来调整市场经济的发展。本文针对我国税收立法存在的问题,试图提出改革的建议。     

《民法典》背景下知识产权惩罚性赔偿制度的路径选择——从国家治理现代化的视角切入

《中华人民共和国民法典》在知识产权领域全面引入了惩罚性赔偿制度。而针对之前商标领域的实证研究却发现,因为在直接公共规制与间接公共规制两种手段之间的选择,知识产权保护与消费维权的情况并不相同,惩罚性赔偿制度在知识产权领域不能得到广泛适用。从国家治理现代化的视角出发,将惩罚性赔偿进行类型化研究并细分为报应性损害赔偿和威慑性损害赔偿两种,并主张由一元选择模式转为二元选择模式,可以回应关于追求报应正义还是矫正正义的原点争论。而借鉴国外经验,并运用新比较经济学中的制度可能性边界模型对知识产权惩罚性赔偿制度的类型化进行阐释,能够说明知识产权惩罚性赔偿制度对直接、间接两种规制手段的依赖关系,进而形成审理不同类型知识产权惩罚性赔偿案件的裁判逻辑,且实现在知识产权保护中的利益平衡。     

法官职业化是全面推进依法治国的重要条件

党的十八届四中全会通过的《中共中央关于全面推进依法治国若干重大问题的决定》提出:建设高素质法治专门队伍,推进法治专门队伍正规化、专业化、职业化。法官是全面推进依法治国的主要实施者,加强法官职业化建设,改革完善法官职业准入制度、法官管理和职业保障制度,提高法官职业化程度,从而形成一支高素质法官队伍,对于实现建设社会主义法治国家目标,有着极其重要的意义。     

A New Anti‐forensic Scheme—Hiding the Single JPEG Compression Trace for Digital Image

To prevent image forgeries, a number of forensic techniques for digital image have been developed that can detect an image's origin, trace its processing history, and can also locate the position of tampering. Especially, the statistical footprint left by JPEG compression operation can be a valuable source of information for the forensic analyst, and some image forensic algorithm have been raised based on the image statistics in the DCT domain. Recently, it has been shown that footprints can be removed by adding a suitable anti‐forensic dithering signal to the image in the DCT domain, this results in invalid for some image forensic algorithms. In this paper, a novel anti‐forensic algorithm is proposed, which is capable of concealing the quantization artifacts that left in the single JPEG compressed image. In the scheme, a chaos‐based dither is added to an image's DCT coefficients to remove such artifacts. Effectiveness of both the scheme and the loss of image quality are evaluated through the experiments. The simulation results show that the proposed anti‐forensic scheme can verify the reliability of the JPEG forensic tools.