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301.
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303.
Identification of a D8S1179 primer binding site mutation and the validation of a primer designed to recover null alleles 总被引:6,自引:0,他引:6
Leibelt C Budowle B Collins P Daoudi Y Moretti T Nunn G Reeder D Roby R 《Forensic science international》2003,133(3):220-227
A population study of Chamorros and Filipinos using short tandem repeat (STR) loci amplified with the AmpFlSTR Profiler Plus PCR amplification kit demonstrated an excess of observed homozygosity at the D8S1179 locus. Use of a different set of D8S1179 primers to type the same samples did not demonstrate an excess of homozygosity and showed discordant genotypes at the D8S1179 locus. A single point mutation, G-to-A transition, 16 nucleotides from the 3' end of the reverse primer, was identified to cause allele dropout when using the AmpFlSTR Profiler Plus primer set. An additional D8S1179 reverse primer specific for the variant was constructed resulting in the recovery of the null allele. The primer was included in the newly developed AmpFlSTR Identifiler PCR amplification kit. No deleterious effects or non-specific peaks were observed in validation experiments evaluating primer concentration, Mg2+ concentration, annealing temperature and population samples. 相似文献
304.
Michael Dennis 《Citizenship Studies》2005,9(2):181-203
Luther P. Jackson was a key supporter of the Association for the study of Negro Life and History and a leading historian of the African American experience. As a leader in the voting rights movement in Virginia as well a prominent activist within region-wide civil rights organizations, Jackson crafted a message of black citizenship that balanced rights and civic duties. His emphasis on political engagement and civic consciousness transcended the specific issues that occupied civil rights activists of the 1940s. This philosophy of political commitment tied the black freedom struggle to the fulfillment of the democratic promise enshrined in the founding documents of the American republic. It also connected the movement for racial justice to the working-class movement for union organization and economic democracy. His effort to place citizenship front-and-center in the civil rights movement echoed the universal ideals of the American crusade to free the world of fascism. It also resonated with the egalitarian aspirations of the Reconstruction era. By linking black equality to political engagement, Jackson set out the only terms under which full equality could be achieved. As much as his message of justice through citizenship challenged the racial orthodoxies of his day, it challenges our contemporary society, transfixed as it is by the illusions of consumerism and marketplace privatization. As Carter G. Woodson and Luther Jackson both understood, racial justice required more than historical consciousness; it required political awareness grounded in a sense of civic responsibility. 相似文献
305.
Since World War II the civic leadership of St. Louis has overcome the extreme fragmentation of public authority by building civic capacity through a variety of strategies and means. Three successive strategies for building civic capacity have unfolded in St. Louis to facilitate the revitalization of the downtown and other large‐scale initiatives. Between 1950 and 1965 a regime strategy was employed in which city hall and the city's corporate elites shared a common vision for urban renewal and the significant national resources that were provided to meet that end. By the mid‐1960s, however, a second strategy for downtown revitalization emerged that featured a corporate‐centered politics during which time the successful assembly of civic capacity hinged largely on the ability of the mayor to present and provide projects in which corporate elites and their companies would be willing investors. Since the early 1990s, building the civic capacity to undertake large initiatives has been made possible through the creation of a constellation of quasi‐public corporations and special districts. This third strategy of “shadow governments” are the most recent means of achieving civic capacity in St. Louis and we argue in this article that these new institutions are transforming the local state because they are capable of forging political coalitions, mobilizing resources, and making decisions that transcend general‐purpose governmental jurisdictions. For cities and for urban regions, the importance of this development is far‐reaching. 相似文献
306.
In this paper we use a simple Downsian spatial model to analyze the properties of campaign contributions. We first consider campaign contributions that are intended to inform voters of candidate positions. We show that it is difficult to construct arguments in a Downsian spatial model for why some voters would choose to contribute to a candidateand the candidate would want to spend the money contributed to inform voters of his position. We then define persuasive campaign expenditures as those that are intended to convince an individual to vote for a candidate regardless of the candidate's position on issues. In the presence of persuasive campaign expenditures some voters have an incentive to contribute to one or both candidates, and the candidates have an incentive to spend the money. We show why the nature of persuasive campaign expenditures may explain both their growth in recent years and the increasing advantage of incumbency. 相似文献
307.
This paper examines the changing relationship between the study of history and the study of political science. It reviews the tensions which produced a divorce between the two subjects, particularly in the United States when behavioural political science was dominant. It then examines five areas in which history has enriched the study of politics: as a source of material; as a demonstration of the links between the present and the past; as a body of knowledge to test theories; as a means of analysing political concepts and as a source of lessons. It concludes that the links between the two subjects today are strong, but that the contribution of history is more as a body of knowledge than as a set of distinctive methods. 相似文献
308.
Dennis O. Grady 《政策研究评论》1988,8(2):322-339
The ability of state leaders to influence economic growth and diversity within their states is a disputed issue within the literature on state economic development policy-making. This research contributes to this debate by developing comparative measures of state development agency power drawn from the emerging theory on organizational power. If state policy leaders have independent control over the economic performance of their states and if that influence is exercised through the administrative unit responsible for that activity, states which have supplied their agencies more resources and freedom in using those resources should outperform those states which have not. 相似文献
309.
Dennis A. Rondinelli 《公共行政管理与发展》1986,6(1):1-21
Governments in developing countries will face serious problems in extending basic social services, public facilities and infrastructure for their rapidly growing urban populations during the next decade. The steadily increasing concentration of the poor in cities will exacerbate already severe strains on urban services. Innovative solutions will be needed to meet the growing demands for urban services. In addition to expanding national and municipal efforts, governments in developing countries must also explore alternative policies and organizational arrangements for meeting the basic needs of their urban population. Among the potential alternatives are: using market surrogates to improve service delivery; lowering the costs of service provision through changes in regulations and controls on urban development; actively supporting self-help and service upgrading schemes by the poor; promoting public-private co-operation and private sector participation in service delivery; increasing effective demand for services by promoting employment and higher incomes; and enacting and implementing policies that attempt to redistribute migration to small and intermediate-sized cities. Each alternative has advantages and limitations that planners and policy-makers must take into consideration in forging effective urban development strategies. 相似文献
310.
For many years, scholars have sought to discover why governments in Western European parliamentary democracies frequently dissolve before the time constitutionally mandated for the holding of elections. Recent research has suggested the potential importance of unpredictable changes in the political environment for bringing about governmental dissolutions. Although not specifically described. the importance of ‘critical events‘ is suggested by both aggregate patterns of dissolutions and anecdotal accounts of particular governments. It is our purpose in this paper to develop further this critical evenrs perspective and evaluate empirically actual governmental histories in light of these developments. Two specific goals are pursued. First, we delineate categories of critical events, thereby grounding the concept of a critical event within existing knowledge of governmental stability. Second, we apply this categorical schema of events to the dissolution of Scandinavian governments during the years 1945–1980. In so doing, we seek not only to assess the adequacy of our conceptualization of events, but also to provide a theoretically and substantively accurate account of the death of modern Scandinavian governments. 相似文献