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251.
This paper is focused on the preparation of samples for laser microdissection (LM) in forensic casework. In forensic genetics, it is essential to preserve and separate cellular traces during sample preparation, as they are usually gathered in very small amounts and are often contaminated with undesired cells. This is made possible by laser microdissection, a technique developed to cut cells or tissue of a certain type from a microscopical specimen by UV laser and catapult them directly into a PCR reactor. This method minimizes the risk of getting inconclusive, mixed DNA profiles due to contamination by foreign DNA and also supplies information about the cellular origin of a DNA profile. A method for optimized fixation and staining of spermatozoa for laser microdissection was established. Four different fixation methods combined with two staining methods were tested on two different microscope slides. Moreover, the effect of a blocker pen to contain the specimen on the slide was investigated. 相似文献
252.
Eric Wilson 《Critical Criminology》2012,20(3):249-274
This essay is a manifesto expounding the relevance of the critical theory of Paul Virilio to critical criminology. I interpret the global credit crisis as a criminogenic ‘event’, explicable in terms of Virilio’s theory of speed-politics. The trans-national space(s) of globalization are inherently criminogenic. ‘Power crime’ is the criminogenic ‘substance’ of global capitalism. Globalization—intensity, extensity, velocity, and impact—equates with cyber-capitalism, which ensures the operational primacy of simulation. Simulation, the fast moving manipulation of post-reality, causes the ‘disappearance of the real’, which underlines the epistemological crisis that attenuates global economic catastrophe. Simulation equates with the ‘logistics of perception’, which manifests itself through both pure war and speed-politics. Simulation and power crime merge on the level of the criminogenic manipulation of reality, resulting in the ‘accident’ of the global credit crisis. Power crime is the criminogenic medium through which the periodic crises of global capitalism will now occur. 相似文献
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This article reports on a systematic review of English language, peer-reviewed publications from 13 empirical studies with donor-conceived children and adults regarding their experiences and perceptions of donor conception. A total of 19 articles that met the inclusion criteria were reviewed. These were identified by means of a bibliographic search of four electronic databases for the period 1990-2011 and supplemented by the authors' personal knowledge of work in this field. No reports from such studies appeared prior to 2000, and more than half have been published since 2008, demonstrating the relative novelty of research in this field. Much of the reviewed research evidence concerns individuals conceived through sperm donation conducted under a regime promoting both anonymity and nondisclosure. Consequently, there is little research that pertains to individuals conceived through other forms of collaborative reproduction, nor to those conceived under arrangements and regimes in which early parental disclosure is both advocated and practised and the identity of the donor and of other genetic relatives may be accessible to donor-conceived individuals. The studies consistently report that most donor-conceived people have an interest in securing information about their genetic and biographical heritage - more information than most of them have been able to obtain. Although a number of methodological limitations in the research base are identified, the authors conclude that the evidence is sufficiently robust to promote the implementation of policies and practices that promote transparency and openness in collaborative reproduction, thus reflecting the importance of maximising future choices and opportunities for donor-conceived people. 相似文献
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Rental vacancy rates: A policy primer 总被引:1,自引:0,他引:1
Eric S. Belsky 《Housing Policy Debate》2013,23(3):793-813
Abstract This article examines how policy makers use rental vacancy rates as indicators of housing market condition. The common practice of assuming that any rental market is in equilibrium when its rental vacancy rate is approximately 5 percent is criticized. Based on theoretical and empirical literature, the case is made that the rental vacancy rate that prevails when the supply and demand for rental units are in balance varies among areas and types of units and is not always 5 percent. The Department of Housing and Urban Development is faulted for not recognizing these variations and, therefore, misusing rental vacancy rates in its formula allocations for the Section 8 and HOME programs. Observed vacancy rates offer meaningful insight into rental market conditions only when they are compared with empirically derived estimates of equilibrium vacancy rates. The methods for estimating these rates, however, are still being refined, and the data needed to derive such estimates are available only for a limited set of metropolitan areas. Other measures are available that policy makers could use to judge the adequacy of low‐income housing supply in local markets, but they are also somewhat problematic. 相似文献
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