对提高公安院校学生逻辑推理能力的思考

公安工作的每一个环节,都与逻辑有不解之缘。逻辑学作为公安院校学生的必修课程,如何通过教学提高学生的逻辑推理能力,是公安院校教学改革中亟待研究的一个课题,必须调整教学内容和教学计划,创新教学方法。     

荧光定量PCR技术研究线粒体DNA nt16519单核苷酸多态性

目的应用荧光定量PCR技术,建立mtDNA nt16519位点的PCR分型方法。方法应用Primer Express3．0软件,设计1对Taqman MGB探针和1埘扩增引物,在探针的5’端分别标记FAM和VIC报告荧光,应用7500荧光定量PCR和直接测序两种方法,分别榆测62份血样和18份毛发样本的mtDNA nt16519单核苷酸多态性,比较二种方法结果的一致性。结果62份血样和18份毛发样本均得到了可靠的mtDNA nt16519分型结果。结论建立的荧光定量PCR的分型方法操作简单、结果准确,适用于法医DNA检案。     

对提高刑事技术实验课教学效果的思考

刑事技术实验课是公安人才教育必不可少的一个教学环节,在公安院校教学中占有比较重要的地位。加强实验室的硬件和软件建设,加强实验室管理,改革实验成绩考核方法,都是提高刑事技术实验课教学效果的有效途径。     

加强公安教育，促进实习工作的落实

实习是公安院校学生实践活动的一个重要方面,它对于提高学生能力,向公安第一线输送高素质公安专业人才具有重要意义,因此学校、实践基地和实习生都应加以重视并予以落实。     

浅析临时禁令与先行判决

及时裁判是民事诉讼程序效率的追求,保证裁判内容实现是提高审判质效的关键。专利侵权类案件一直存在审理周期长,无法及时保护权利人利益的诟病,即使对侵权事实部分适用先行判决,也因诉讼尚未终结无法获得执行力。深圳市中级人民法院在“大疆公司专利纠纷案”中首次引入“先行判决+临时禁令”的裁判方式,有效形成先行判决权利变现的制度合力,保障权利人及时止损。     

Study on Mechanism of SOE Classified Governance ——Based onthe Perspective of Agency Costs

This paper starts from the different types of corruption in SOE, constructs the internal mechanisms of five kinds of agency-cost dissimilation that influence the SOEs performance, and based on the data of Chinese state-owned listed companies from 2008 to 2015, classifies and measures these agency costs. This study shows that the major problems of power renting, on-the-job consumption and related transactions for private benefits bring significant negative effect to the enterprise performance. Social responsibility is likely to damage the short-term interests of the internal stakeholders, but will increase the media exposure that may improve the enterprise performance via external supervision and constraint. Furthermore, the CEO duality in SOEs and the increasingly close political associations will enlarge the effect of agency cost on business performance, but the influence mechanisms are different in the measurement of classified SOEs. This research offers a new theoretical perspective to the SOE reform and classified governance.     

苏联学者对马克思主义法学的两种理解考辨

马克思主义法学思想与中国法治建设的关系,在学术界有否定性意见,问题的产生与源自苏联并影响中国的两种理论解读相关。一个是帕舒卡尼斯囿于“商品交换法学”和未来共产主义社会中法的消亡原理,而否认社会主义法制建设的法律虚无主义;一个是维辛斯基强调法的阶级性而偏于意志论的法律工具主义。前者使社会主义国家趋于人治和以政代法,后者导致对法的过度意识形态化。其实,马克思主义法学的最根本之点在于坚持历史唯物主义立场,强调法律源于人类生产活动,并以其对生产力发展的正负面影响而使社会进步或倒退。马克思主义法学在改革开放的中国一直在场,有必要从意义域角度总结马克思主义法学发展的得失。     

Combating Domestic Violence During COVID-19: What Does the Chinese Experience Show Us?

Previous evidence demonstrates that a public health crisis triggers and intensifies domestic violence, especially against women. The pandemic of COVID-19 supported this observation, as the world witnessed an increase in domestic violence. China has witnessed a similar trend. While the Chinese government is applying a stringent model of virus control measures over COVID-19 when compared to certain other countries, many questions remain: How is this impacting the situation of domestic violence in China? What has China done to combat domestic violence during COVID-19? What can we learn from the Chinese experience? This article intends to answer these questions by reviewing reports and media coverage and conducting interviews with stakeholders. First, it examines causes of domestic violence during COVID-19. Second, it analyses the types and features of domestic violence during COVID-19. Third, the article intends to discover the current difficulties in combatting domestic violence. Fourth, it presents good practices for specific local areas. Last, it concludes with recommendations for tackling domestic violence during public health crises, such as the COVID-19 pandemic, by understanding the Chinese experience.     

神经肽Y Y_1受体在发情周期大鼠子宫中的分布

选择健康、未产、体重220～250g的成年雌性SD大鼠,采用阴道涂片方法鉴定其发情周期,并采用免疫组织化学SP法研究了神经肽YY1受体在大鼠发情周期子宫内分布的变化规律。结果显示,各期子宫内膜的神经肽YY1受体免疫阳性产物主要位于基质细胞核膜、子宫内膜上皮细胞质以及子宫腺细胞质和核膜中,并且着色深浅有一定差别,发情间期着色最深,发情后期、发情前期、发情期依次减弱;在肌层和外膜中,发情各期的神经肽YY1受体免疫阳性产物主要位于肌细胞核中,还有一小部分位于结缔组织细胞中,并且在发情间期着色最深,发情前期中等强度着色,发情期和发情后期着色最浅。表明,神经肽Y可能通过神经肽YY1受体参与子宫生理功能的调节。     

猪圆环病毒Ⅰ型ORF1基因的克隆及其在大肠杆菌中的表达

根据GenBank中猪圆环病毒Ⅰ型(PCV1)ORF1基因序列,设计合成了1对引物,对PCV1 ORF1基因进行PCR扩增,将扩增出的ORF1基因(939 bp)克隆入pMD18-T载体,将筛选获得的重组质粒命名为pMD-ORF1。测序分析表明,克隆的ORF1与广东分离株DQ659154的核苷酸序列同源性高达99.6%,推导的氨基酸序列同源性为99.4%。将ORF1双酶切产物插入原核表达载体pET-41a,得到的重组子命名为pET-ORF1。用IPTG进行诱导表达,收集菌液进行SDS-PAGE和Western-blot分析,结果表明,PCV1ORF1在pET-41a中获得了高效融合表达,其表达蛋白的分子质量约为67.6 ku。