9个Y-STR基因座荧光复合扩增系统的法医学应用

目的建立9个Y-STR基因座的复合扩增系统,提高Y-STR的法医学检测效能。方法6-FAM标记DYS434、Y-GATA-A10、DYS438、DYS439,HEX标记DYS531、DYS557、DYS448,TAMRA标记DYS456、DYS444引物,PCR复合扩增,毛细管电泳得到结果,考察扩增系统的个体识别能力、灵敏度、特异性、组织同一性。结果所建立的9个Y-STR复合扩增系统分型清晰,单倍型多样性达0.9968,特异性好,灵敏度高(0.5ngDNA),并且在男女混合斑检验上较常染色体STR分型更有优势。结论9个Y-STR复合扩增系统具有较高的识别能力,对建立Y染色体STR数据库,研究群体遗传学和进行法医学混合斑物证鉴定有重要意义。     

Analysis of 15 STR loci in Chinese population from Sichuan in West China

Allele frequencies for 15 STR loci found in PowerPlex 16 System kit were determined in a sample of 200 unrelated individuals living in Sichuan area in west China. The values of observed heterozygosity (Ho), power of discrimination (PD), probability of paternity exclusion (PE) and polymorphism information content (PIC) were calculated. All loci were in accordance with Hardy-Weinberg equilibrium (p<0.05). The obtained frequency distributions were compared with other previously reported population data.     

烧骨组织形态变化及DNA技术在个体识别中的应用

烧骨在火灾、焚尸、交通、爆炸等案件和事故的检材中具有特殊的地位。通过对不同条件焚烧下烧骨组织形态及DNA变化规律的研究,可为法医实践中烧骨的种属鉴定、性别及年龄判定提供准确的依据和标准,同时可利用残存的基因位点对烧骨残块进行个体识别和同一认定。烧骨DNA的提取方法及检测技术也在不断探索和改进。本文对烧骨在形态学、组织学和分子生物学水平研究进展以及烧骨评测的方法、技术进行概述,旨在为法医实践及进一步研究提供新的方法和思路。     

法律修辞在中国兴起的背景及其在司法审判中的作用

中国的社会转型使我们处于一个高度不确定的风险环境中,这为以不确定性因素为逻辑起点的法律修辞研究提供了丰富的现实土壤。但法律修辞学在中国学界被热捧的理论基础,并不像它在西方社会复兴时那样坚实。当前中国社会并没有经历法律形式主义极度发达的严格法治时代,没有形成严格守法的习惯,缺乏遏事遵守规则的意识。因此,立足于省略三段论推理、强调理性说服功能的法律修辞研究,必须在崇尚程序规制的法律论证框架下使用,才能坚持司法审判的首要目标是依法正确判决,然后才是通过修辞的合理包装送达当事人和社会。     

A 21‐Locus Autosomal SNP Multiplex and its Application in Forensic Science

To develop a cost‐effective technique for single‐nucleotide polymorphism (SNP) genotyping and improve the efficiency to analyze degraded DNA, we have established a novel multiplex system including 21‐locus autosomal SNPs and amelogenin locus, which was based on allele‐specific amplification (ASA) and universal reporter primers (URP). The target amplicons for each of the 21 SNPs arranged from 63 base pair (bp) to 192 bp. The system was tested in 539 samples from three ethnic groups (Han, Mongolian, and Zhuang population) in China, and the total power of discrimination (TPD) and cumulative probability of exclusion (CPE) were more than 0.99999999 and 0.98, respectively. The system was further validated with forensic samples and full profiles could be achieved from degraded DNA and 63 case‐type samples. In summary, the multiplex system offers an effective technique for individual identification of forensic samples and is much more efficient in the analysis of degraded DNA compared with standard STR typing.     

公民网络参与视角下司法的回应

当下我国司法领域面临着深刻的信任危机,公民通过网络参与司法运作是实现公民参与权等相关权利的重要路径,也是实现司法公正的前提条件,具有正当性。但公民参与司法运作的权利也要受到一定的限制,因为公民的言论自由与司法权存在着冲突,公众的知情权与个人的隐私权也存在着冲突。公民的网络参与一方面增强了司法的民主性,另一方面网络舆论所形成的压力还影响着司法权威的生成。因此,司法机关也应建立起理性的回应机制,对网络舆论进行实时监控,及时引导和应对网络舆论。     

浅析车辆超载超限运输的现状和整治对策

超载超限运输已对道路交通安全构成严重危害。分析超载超限运输现状,整治超载超限运输工作有五个方面的难点。治理超载超限运输,一要发挥职能部门作用,合理调节运输价格;二要加强联动,落实安全生产责任制;三要建立管理长效机制,从源头上遏制超载超限;四要全国一盘棋,整体实现计重收费,体现管理的公平公正;五要加大执法力度,提高违法成本,建立治超保障机制。     

Using Fuzzy Rule–Based Systems to Evaluate Overall Financial Performance of Governments: An Enhancement to the Bond Rating Process

Credit ratings remain a key feature of municipal debt management. The primary objective of this article is to develop a new methodology for evaluating the financial performance and creditworthiness of governments and to illustrate this approach for a sample of large American cities. Specifically, we develop a fuzzy rule–based system (FRBS) that uses economic, debt, and other financial information as well as a measure of financial management to produce rankings of city financial performance. The FRBS credit ratings are highly correlated with actual Moody's ratings for these cities. FRBS have the potential of enhancing the rating process by standardizing the information used and encouraging consistent rules about what combinations of inputs result in good, fair, or poor performance.     

Do state balanced budget requirements matter? Testing two explanatory frameworks

Balanced budget requirements (BBRs) affect all aspects of financial operations. Previous studies relied on characterizations that highlight a constitutional-statutory distinction. Hou and Smith (Public Budgeting & Finance 26(3):22–45, 2006) instead propose a political-technical construct. This article uses probit estimation, six measures of balance, and long panels to test which framework offers more explanatory power. The findings suggest that BBRs matter to varying degrees. Technical requirements exert bigger effects than political ones, the effects are more obvious on narrower than broader measures of balance and in the later phases of the budget cycle, and the political-technical construct offers more explanatory power than the constitutional-statutory distinction.     

乡镇概念:内涵与特征

在一般意义上,乡镇有两种解释,一是从行政区划的角度解释,二是从城镇的角度解释。乡镇的基本特征,可以根据对乡镇基本内涵的两种解释,来分别进行探究。