多头带绦虫六钩蚴Tm45W基因的克隆表达与免疫原性分析

为分析多头带绦虫Tm45W重组蛋白的免疫原性,利用RT-PCR技术首次从激活的多头带绦虫六钩蚴中扩增出多头带绦虫Tm45W基因。将扩增产物重组于原核表达载体pET32a(+)中,构建pET32a-Tm45W表达载体,转化至大肠杆菌BL21(DE3)中,经IPTG诱导表达融合蛋白;表达产物进行SDS-PAGE和Western-blot分析后,将纯化的Tm45W重组蛋白免疫小鼠,用ELISA检测其血清抗体。结果显示,Tm45W基因全长为813bp,其开放阅读框为765bp,编码255个氨基酸,与多头带绦虫新疆株多头蚴45m基因(序列号:EU326106)的部分序列同源性为90.60%;表达的重组蛋白大小为45ku,与脑多头蚴病羊血清的免疫印迹分析呈阳性反应;小鼠免疫后第1周血清中即可检测到抗Tm45W蛋白的特异性抗体,并于免疫后第35天达到高峰。结果表明Tm45W重组蛋白具有较好的反应活性,能引起机体较强的体液免疫反应。     

Medicare program; Medicare Shared Savings Program: Accountable Care Organizations. Final rule

This final rule implements section 3022 of the Affordable Care Act which contains provisions relating to Medicare payments to providers of services and suppliers participating in Accountable Care Organizations (ACOs) under the Medicare Shared Savings Program. Under these provisions, providers of services and suppliers can continue to receive traditional Medicare fee-for-service (FFS) payments under Parts A and B, and be eligible for additional payments if they meet specified quality and savings requirements.     

Medicare and Medicaid programs: hospital outpatient prospective payment; ambulatory surgical center payment; hospital value-based purchasing program; physician self-referral; and patient notification requirements in provider agreements. Final rule with comment period

This final rule with comment period revises the Medicare hospital outpatient prospective payment system (OPPS) for CY 2012 to implement applicable statutory requirements and changes arising from our continuing experience with this system. In this final rule with comment period, we describe the changes to the amounts and factors used to determine the payment rates for Medicare hospital outpatient services paid under the OPPS. In addition, this final rule with comment period updates the revised Medicare ambulatory surgical center (ASC) payment system to implement applicable statutory requirements and changes arising from our continuing experience with this system. In this final rule with comment period, we set forth the relative payment weights and payment amounts for services furnished in ASCs, specific HCPCS codes to which these changes apply, and other ratesetting information for the CY 2012 ASC payment system. We are revising the requirements for the Hospital Outpatient Quality Reporting (OQR) Program, adding new requirements for ASC Quality Reporting System, and making additional changes to provisions of the Hospital Inpatient Value-Based Purchasing (VBP) Program. We also are allowing eligible hospitals and CAHs participating in the Medicare Electronic Health Record (EHR) Incentive Program to meet the clinical quality measure reporting requirement of the EHR Incentive Program for payment year 2012 by participating in the 2012 Medicare EHR Incentive Program Electronic Reporting Pilot. Finally, we are making changes to the rules governing the whole hospital and rural provider exceptions to the physician self-referral prohibition for expansion of facility capacity and changes to provider agreement regulations on patient notification requirements.     

Medicare program; availability of Medicare data for performance measurement. Final rule

This final rule implements Section 10332 of the Affordable Care Act regarding the release and use of standardized extracts of Medicare claims data for qualified entities to measure the performance of providers of services (referred to as providers) and suppliers. This rule explains how entities can become qualified by CMS to receive standardized extracts of claims data under Medicare Parts A, B, and D for the purpose of evaluation of the performance of providers and suppliers. This rule also lays out the criteria qualified entities must follow to protect the privacy of Medicare beneficiaries.     

TRICARE: unfortunate sequelae from noncovered services in a military treatment facility. Final rule

The Department of Defense is publishing this final rule to allow coverage for otherwise covered services and supplies required in the treatment of complications (unfortunate sequelae) resulting from a noncovered incident of treatment provided in a Military Treatment Facility (MTF), when the initial noncovered service has been authorized by the MTF Commander and the MTF is unable to provide the necessary treatment of the complications. This final rule is necessary to protect TRICARE beneficiaries from incurring financial hardships due to the current regulatory restrictions that prohibit TRICARE coverage of treatment of the complications resulting from noncovered procedures, even when those procedures were conducted in a Department of Defense facility.     

Medicare program; hospice wage index for fiscal year 2012. Final rule

This final rule will set forth the hospice wage index for fiscal year (FY) 2012 and continue the phase-out of the wage index budget neutrality adjustment factor (BNAF), with an additional 15 percent BNAF reduction, for a total BNAF reduction in FY 2012 of 40 percent. The BNAF phase-out will continue with successive 15 percent reductions from FY 2013 through FY 2016. This final rule will change the hospice aggregate cap calculation methodology. This final rule will also revise the hospice requirement for a face-to-face encounter for recertification of a patient's terminal illness. Finally, this final rule will begin implementation of a hospice quality reporting program.     

环境侵权法律制度对环境责任保险的影响

环境侵权责任风险是环境责任保险产生的前提和基础。环境责任法律的具体制度安排及其执行,对环境责任风险的大小及其可预测程度产生了重大影响。我国现有环境侵权法律存在因果关系证明严苛、赔偿制度不健全、公益诉讼制度缺乏以及法律执行不力等缺陷,致使环境污染企业因侵权责任风险太小而普遍缺乏购买责任保险的内在动力,严重制约着环境责任保险制度的发展。     

推导作为诉讼证明的逻辑

关于证据与证明的传统哲学与逻辑理论存在着实践缺陷,形式逻辑中的演绎和归纳推理不能合理解释依证据进行的事实认定。证据的作用不可能是回复案件真相,而只是为特定假设提供支持。推导作为一种可废止和情境化的第三种类型的推理是契合诉讼事实认定的逻辑形式,这种内容求真的推理与形式逻辑上的演绎及归纳推理有着本质的区别。推导理论的提出会对传统证据法理论形成冲击,从这一角度我们会对证明标准与证明责任、直接证据与间接证据、表见证明与摸索证明、事实推定与经验法则等概念与问题形成新的认识。理解案件事实认定过程的推导本质,并对其缺陷有足够的认识,才能在实践中谨慎论证与比较各种假设,以全面的信息检验假设以避免最终的误认。     

法律现实主义与转型社会刑事司法

我国社会正处于转型期,各种社会矛盾凸显,刑事司法面临着新的挑战。西方的法律现实主义能为我国转型期刑事司法提供可资借鉴的方法论和改革思路。法律现实主义运动在批评形式主义与三段论式审判的基础上,把法律看作具有多重目标的社会引擎,关注司法行为、纠纷解决与社会问题。在中国语境下,引入法律现实主义,不是要简单地否定形式主义,而是应理性对待形式主义并在其中融入客观主义;应在刑事司法裁判中适用利益权衡,重视经验归纳而不拘泥于简单的演绎推理;应以实现社会正义为核心,构建合理衔接的多元纠纷解决机制。     

4种影像学检查方法应用于肩关节损伤法医学鉴定的比较

目的 评价X线、CT、MRI及CT气-碘双对比造影4种方法在肩关节损伤法医学鉴定中的应用价值.方法通过对肩关节损伤患者行X线摄片、CT扫描、MRI成像与CT气-碘双对比造影检查,观察、分析4种方法所得的影像学资料.结果 X线摄片对骨折的诊断率为52.8%,对软组织损伤的诊断率为0%;CT对骨折的诊断率为72.0%.对软...