ABO基因分型与多重STR联合检测在法医学中的应用

目的建立ABO基因型和Goldeneye16A试剂盒联合检测的方法,并评价其在法医学实践中的应用价值。方法将6种ABO基因型(A/A,A/O,B/B,B/O,A/B,O/O)的序列特异性引物(PCR-SSP)检测方法与Goldeneye16A试剂盒相整合进行同步分型。通过对460份男性个体血痕样本、9947A DNA及90份案件样本进行检测,考察方法的一致性、灵敏度及对法庭科学检材的适用性。结果应用本文方法可同时检出6种ABO基因型和15个常染色体STR基因座及性别决定基因座,检测灵敏度为125pg,其中ABO基因检测灵敏度达63pg。460份男性血痕和90份案件检材证实该联合分型方法用于各类检材结果准确、稳定。结论本文ABO基因分型与多重STR联合检测方法,适用于各类含有核细胞的生物检材,在法庭科学DNA鉴定中有较好的应用前景。     

五色荧光标记20个基因座复合扩增体系及法医学应用

目的建立20个基因座五色荧光标记复合扩增检测体系,并评价其法医学应用价值。方法收集368份无关人血样及55份实际案例样本(包括血斑、体液斑、组织及毛发),采用五色荧光素标记技术,对Amelogenin和19个STR基因座(D19S433、D5S818、D21S11、D18S51、D6S1043、D3S1358、D13S317、D7S820、D16S539、CSF1PO、Penta D、vWA、D8S1179、TPOX、Penta E、TH01、D12S391、D2S1338和FGA)进行基因型检测,并考察方法的一致性、灵敏度、种属特异性及检材适用性。结果本文五色荧光标记复合扩增检测体系可对所选20个基因座分型,结果稳定准确,且均衡性良好、无杂峰;群体调查显示累积个人识别率和累积非父排除率分别是0.999 999 999 999 999 999 999和0.999 999 99;灵敏度达125pg,种属特异性高,实际案例检材分型成功率高。结论本文五色荧光标记复合扩增检测体系各项指标可达到当前商品化试剂盒的检测水平,具有重要的法医学应用价值。     

法医SNP复合检测体系的构建及应用

目的构建48-SNP位点复合检测体系,用于个体识别、性别鉴定、ABO基因分型。方法采集225份无关个体样本(血斑及口腔拭子),18份案例样本(不同组织及体液斑),选择43个常染色体位点、4个ABO基因位点和1个性别鉴定位点,根据单碱基延伸技术通过GenomeLabTMSNPstream基因分型系统进行SNP分型;并检测体系灵敏度、同一个体不同组织同一性及模拟腐败检材。结果 48-SNP体系分型结果与测序结果的一致性为100%,最小DNA检出量为0.25ng,不同组织来源样本检测同一性很好;利用该体系检测225名无关汉族个体,所有位点均符合Hardy-Weinberg平衡,整个系统的随机匹配概率为9.4×10-18,累积非父排除率(CEP)为0.999 788,累积个体识别率大于0.999 999 999 999 999 99。结论本文48-SNP体系能同时进行个体识别、ABO基因分型和性别鉴定,可以作为现有STR检验体系的补充。     

我国量刑建议制度的建构

量刑建议制度的实行有利于发挥检察机关的审判监督职能,拓展庭审的辩护空间,约束法官的自由裁量权。完善我国的量刑建议制度应规范量刑建议的提出主体,量刑事实的调查取证,严格掌握量刑建议的具体幅度,规范量刑建议的提出程序,并加强内部监督,避免量刑建议权的滥用。     

Diverse morphological lesions and serious arrhythmias with hemodynamic insults occur in the early myocardial contusion due to blunt impact in dogs

To investigate the morphology and hemodynamics of the early myocardial contusion, an animal model of cardiac contusion was established by impact to the precordial region at sternum at velocity of 10.0m/s with a mechanical elastic-cord propelled impactor in 19 dogs. The electrocardiogram and both the left and right intra-ventricular pressures were recorded continuously throughout the experiment. Histological and immunohistochemical examinations of myoglobin, creatine kinase-MB and fibrinogen were conducted. At the moment of impact, abrupt over-pressures within the left and right ventricles occurred with concomitant serious arrhythmias followed by variety of cardiac conduction disorders and depressed left and right ventricular systolic pressures during the observation times. Histologically, lesions of myocardial contusions were identified at subepicardial, myocardial or subendocardial layer as interstitial hemorrhage, disruption or coagulative necrosis as well as contraction band necrosis of the muscle fibers, which might be categorized into the hemorrhagic, necrotized and mixed forms. The three forms of lesions were found to exist independently, or co-existed in a heart. However, severity of the lesions varied greatly with different parts even within a heart. Intravascular thromboses were occasionally discovered post-impact. Immunohistochemically, loss of myoglobin and creatine kinase-MB from cardiac cells, and accumulation of fibrinogen at the cell membranes were detected 5min post-impact. The intracellular accumulation of fibrinogen increased with extension of post-impact intervals. Our results indicate that diverse morphological lesions concomitant with hemodynamic compromise and serious, even fatal arrhythmias occur in the early myocardial contusion, and intravascular thromboses are occasionally produced, suggesting that traumatic myocardial ischemic lesion may be induced due to blunt impact to the precordial region.     

何以“藕断续连”:高校共青团的文化生态与青年话语体系建设

基于文化生态的视角审视和把握高校共青团文化建设的基因组合与职能重塑对于推进高校共青团组织积极构建与青年群体特点相契合、与青年成长规律相适应的“青年话语表达体系”具有重要方法论意义.消解文化现代性对高校共青团造成的文化冲突和文化对立,就要充分发挥高校共青团干部的主观能动性,推进青年学生思想政治教育话语表达体系的变革,将情感元素、教育元素、专业元素植入共青团活动的创作理念之中,以期达到“润物细无声”的思想育人目的.     

推进社会主义核心价值观青年化的三重维度

构建社会主义核心价值观青年化的实现路径,要加强政党的宏观调控,把握高校的空间特点,要构建青年话语的表达体系,发挥共青团的媒介作用,增强社会主义核心价值观对青年的吸引力、感染力和影响力。     

东方海上丝绸之路视角下的中韩合作探析

东方海上丝绸之路古已有之,源远流长。这条古代东方海上丝绸之路在2013年重新焕发出新的活力。沿着这条东方海上丝绸之路,中韩两国互利合作,硕果累累,于2015年签署自由贸易协定。在此利好基础上,中韩两国应当继续携手合作,奋发作为,将中国的"一带一路"战略与韩国的"欧亚倡议"充分有效对接,让中韩之间的这条东方海上丝绸之路成为两国世代友好、共生共赢的纽带。