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161.
通过了解MDCK细胞感染犬细小病毒(CPV)后基因表达水平的差异,研究病毒对细胞的致病作用以及细胞抵御病毒感染的机制。利用表达谱基因芯片技术,分析持续感染犬细小病毒的MDCK细胞基因表达水平的变化情况,并用Real-Time PCR技术加以验证。结果显示,获得了359个差异大于1.5倍的表达基因(P0.05),占总基因数的1.53%,其中193个上调表达(0.84%),166个下调表达(0.69%)。对差异基因进行GO功能聚类分析,小部分涉及免疫应答、生长周期调控、信号转导和蛋白酶活性,其他大部分基因功能未知。利用Real-Time PCR随机验证5个基因在持续感染CPV后的差异表达,其结果和芯片杂交的结果一致。表明建立了MDCK细胞持续感染CPV后的差异表达谱,初步了解到CPV对宿主细胞的制约作用,引起部分细胞增殖调控相关基因发生了表达下调,以及细胞对感染的积极应答反应,部分免疫反应基因、肽链内切酶活性基因等发生了上调表达,从而为探索病毒的致病机理和宿主的抗病毒途径提供了试验基础。 相似文献
162.
Kimberly L. Barrett Michael J. Lynch Michael A. Long Paul B. Stretesky 《American Journal of Criminal Justice》2018,43(3):530-550
Studies that assess the impact of monetary penalties on environmental compliance have yielded mixed results. While some studies suggest fines deter future violations other studies find that fines do little to encourage compliance. This longitudinal study examines the impact of the dollar amount of fines on compliance with environmental laws among major facilities in the state of Michigan (n?=?37). Results from a mediation analysis suggest that while noncompliance may slightly decrease immediately following a fine there are few changes to a firm’s long term compliance behavior. Furthermore, analyses of these data suggest that total fines levied prior to the most recent fine actually have a positive relationship with noncompliance. We suggest these results imply a decaying effect of deterrence that is perhaps connected to the organizational structure of the treadmill of production. 相似文献
163.
运用统计学理论,动态分析中国农村居民的消费结构及影响因素,可为提高农村居民的生活质量提供参考。[1]研究结果表明:1990年以来,农村居民恩格尔系数处于递减的趋势,食品、衣着比例减少,医疗、交通和通讯比例增加,消费结构有所改善,但2005年以后,农村居民在文教娱乐方面的支出比重有下降趋势,此现象值得关注。建议通过提高农民科学素养、拓宽增收渠道等措施增加农民收入,促进消费结构的优化。 相似文献
164.
GPC-GC/MS法分析血中20种安眠镇静药物 总被引:1,自引:0,他引:1
目的研究建立液液提取-在线GPC-GC/MS同时分析血中巴比妥类、吩噻嗪类、苯并二氮杂卓类、三环类和其他安眠精神类药物的方法。方法血样加入内标SKF525A,加β-葡萄糖酸苷酶水解后用乙腈沉淀蛋白浸取药物,加无水硫酸镁脱水,提取液浓缩至干后用GPC流动相定容,在线GPC净化,大体积进样,GC-MS分析。结果选择总离子监测模式,血中20种安眠镇静药物提取率在80.2%~99.3%之间,检出限小于21.6ng/mL;在0.1~10μg/mL浓度范围内工作曲线的线性关系良好。结论方法可用于安眠镇静类药物误服中毒者和刑事案件中毒者血样的分析。 相似文献
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166.
Gang-affiliated youth are responsible for a disproportionate amount of serious and violent offenses. However, there is scant focus on the psychological variables that could be important for treatment planning and program development. Awareness of these variables is important for treatment planning and program development and also, potentially, for understanding which youth may be attracted to gangs. This study compared the criminal attitudes and psychopathic personality attributes of gang- and nongang-affiliated youth offenders (N=168) residing in youth correctional institutions in Singapore. Multivariate analyses indicated that favorable attitudes toward gangs (ATG), violence, and criminal associates remained significantly associated with gang membership after accounting for favorable attitudes toward violence and criminal associates, as well as increased attitudes of entitlement and impulsive/irresponsible traits. These attitudes were likely to perpetuate gang affiliation and criminal behavior, and thus should be a focus for intervention and rehabilitation efforts. 相似文献
167.
Michelle Ann McManus Matthew L. Long Laurence Alison Louise Almond 《Journal of Sexual Aggression》2013,19(3):368-384
AbstractThis study explored a sample of 244 UK adult male offenders convicted of offences involving indecent images of children (IIOC): 120 had a previous contact child sexual offence (defined as dual offenders) and 124 had no evidence of an offence against a child (defined as non-contact offenders). Offender groups were compared regarding their socio-demographic characteristics, previous convictions and IIOC possession. Key discriminatory factors that differentiated dual offenders were: access to children, previous offence history, sexual grooming and possession of IIOC that depicts similar-aged victims. In contrast, non-contact offenders could be identified from their greater amount and wider range of IIOC possession. The results suggest a homology between Internet behaviours, IIOC possession and victim selection. Implications for law enforcement agencies are discussed in terms of assisting investigative prioritisation by identifying those most at risk of committing sexual abuse against children. 相似文献
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170.
Ngô Vĩnh Long 《当代亚洲杂志》2013,43(2):295-304
This paper analyses the growth trajectory of China and related structural change to assess China's capacity to continue its rapid growth over the next decade. The evidence demonstrates that the multi-path approach undertaken has enabled China to transform its economy from low value-added towards high value-added activities through structural change from low to high value-added industries, as well as upgrading within industries. In doing so, China did not follow the neo-liberal advocacy of freeing markets. Selective state interventions facilitated China's transformation from an agricultural to an industrial economy over the last few decades. Upgrading towards higher value-added activities and the continuing strength of macroeconomic indicators, such as balance of payment and capital account surpluses, and low trade intensity of GDP and debt service along with significant deepening in human capital and R&;D activities, suggests that China will continue to grow relatively rapidly over the next decade. To do this China needs to find solutions to growing deficits in power and water supply, and potentially dangerous political upheavals if growing economic inequality problems are not solved. 相似文献