"三个代表"重要思想的哲学意义

"三个代表"重要思想具有坚实的马克思主义哲学基础,它揭示了"生产力和生产关系、经济基础和上层建筑"的辩证关 系;强化了"社会主义物质文明和精神文明"的辩证关系;体现了"社会主义经济、政治与文化"的辨证统一;是相互联系、相互促进、不 可分割的辩证统一体。     

着眼群众艺术——陈云与评弹

陈云同志被评弹界称为“老听客”。陈云对评弹工作的关心和指导，不仅仅因为个人爱好，更是因为评弹是人民大众文艺的一部分。这种艺术在吴语区域内联系着千千万万群众。同时，他也认为这种形式是曲艺比较成熟的曲种，有较强的艺术感染力，有必要加以提倡和推广。,-年代，他在杭州和上海、苏州等地，利用公余和疗养时间，听了大量的评弹书目，广泛接触各种流派的评弹艺人、创作人员和领导干部，对评弹艺术进行了深入的调查研究，不断对其中的一些重要问题，发表了独具见解的意见。./,/年..月0,日至01日，陈云在杭州谢家花园召开了建国后评弹…     

执政党的功能定位与加强党的执政能力建设

在现代社会发展过程中,党、国家和社会都在不同程度上获得了各自独立性,执政党必须明确政党的功能,正确的处理与国家、社会的关系,避免做自己不该做,也做不好的事的现象,从执政党的功能上,加强执政能力建设.     

邓小平的发展观探究

邓小平的发展观蕴涵着丰富的内容。从总体上看,主要体现在以下五个方面：提出三个文明建设,谋求总体的全面协调可持续发展;提出共同富裕的构想,探求社会领域内的全面协调发展;重塑民主精神,图求政治领域内的全面协调和以人为本的发展;提出速度、质量与效益的统一,追求经济领域内的协调可持续发展;提出南南合作、南北合作的构想,以求实现国际领域内的全面协调可持续发展。     

论对抽象行政行为的司法审查

在我国立法中排除了人民法院对抽象行政行为的司法审查,但为保护受违法抽象行政为侵害的行政相对人的利益,对抽象行政行为进行司法审查是必要也是可行的。     

《治安管理处罚法》实施中的初步成效与问题━━对四川省部分地区的调研

《治安管理处罚法》自2006年3月1日实施以来,在违法行为整治、警察权力规范等方面初步显示出其法制效力。但与此同时,也显现出一系列的新情况、新问题,如治安处罚、民警执法、传唤未成年人证人和通知家属等方面都出现了一些新问题,有待《公安管理处罚法》的完善。     

对我国在建设责任政府过程中加强居安思危未雨绸缪能力建设的思考

建设责任政府是我国转变政府职能的重要内容,而要建立责任政府,就不能仅仅关注于对问题的解决及出现问题后的责任追究,它还必须更关注于对问题的预防,对潜在危机的消除。这就必须加强政府居安思危、未雨绸缪的能力建设。为此,政府不仅要提高领导者的相关素质,更重要的是要创新体制和机制,要在建立有效的政策监督、评估机制、建立权责明确的组织体系、加强政策研究机构建设等方面加大力度。     

中华民族精神与和谐社会建设问题研究

深入研究和探讨中华民族精神与和谐社会建设的关系,大力弘扬中华民族精神,为构建社会主义和谐社会服务,是新世纪新阶段党的民族工作必须关注的重要问题之一.     

网络传播伦理原则简论

网络传播的飞速发展给传播伦理带来了巨大的冲击.如何充分认识网络传播的特有规律,建构网络传播中的伦理规范,已成为我国网络传播研究的主要问题.网络环境下媒体伦理的建设要通过社会和媒体及广大网民的共同努力、外部强制和个人自律、道德引导和法律控制的多方位多层次的建构策略,才能建立和完善网络传播伦理,使网络传播良性有效地运行.     

Application of HLA-DRB1 genotyping by oligonucleotide micro-array technology in forensic medicine

The human leukocyte antigen (HLA) system is known to be the most complex polymorphic system in the human genome. Among all of the HLA loci, HLA-DRB1 has the second largest number of alleles. The purpose of this study is to develop an oligonucleotide micro-array based HLA-DRB1 typing system for use in forensic identification, anthropology, tissue transplantation, and other genetic research fields. The system was developed by analyzing the HLA-DRB1 (DRB1) genotypes in 1198 unrelated healthy Chinese Han individuals originating from various parts of China and residing in Shanghai, China. METHOD: Polymerase chain reaction (PCR) coupled with the oligonucleotide micro-array technology was used to detect and type HLA-DRB1 alleles of the sample individuals. The reliability, sensitivity, consistency and specificity were evaluated for use in forensic identification. Furthermore, a meta-analysis was carried out by comparing the allele frequencies of the HLA-DRB1 locus with those of other Chinese Han groups, Chinese minorities and other ethnic populations. RESULTS: All the DNA samples yielded a 273 bp amplification product, with no other amplification products in this length range. The minimum quantity of DNA detected by this method is 15 ng in a PCR reaction system of 25 microl. The population studied appeared to be not in Hardy-Weinberg equilibrium. Observed heterozygosity (Ho), expected heterozygosity (He), expected probability of exclusion (PE), polymorphic information content (PIC), and discrimination power (DP) of the HLA-DRB1 locus from the Shanghai Han ethnic group were evaluated to be 0.8022, 0.8870, 0.7741, 0.8771, 0.9750, respectively. A total of 25 HLA-DRB1 alleles were identified. HLA-DRB1*09XX, *04XX, *12XX and *15XX were the most frequent DRB1 alleles, which were observed in 58.76% of the sample. One hundred and sixteen genotypes were found. The five most frequent genotypes were: *04XX/*04XX (0.0626), *09XX/*09XX (0.0593), *04XX/*09XX (0.0551), *09XX/*15XX (0.0384) and *08XX/*12XX (0.0351). The meta-analysis showed that there were uniquely distributed features of DRB1 alleles among various ethnic populations and among the studied population groups from various regions with the same ethnic origin. CONCLUSIONS: An HLA-DRB1 genotyping system has been developed and established based on the oligonucleotide micro-array technology. The HLA-DRB1 typing of the Han population in Shanghai has revealed a relatively high heterogeneity. Information obtained in this study will be useful for medical and forensic applications as well as in anthropology research. Large-scale micro-array detection is highly accurate and reliable for DNA-based HLA-DRB1 genotyping. These results suggest that HLA-DRB1 DNA polymorphisms and the database of the Shanghai Han group have useful applications in processing forensic casework (as personal identification, paternity test), tracing population migration and genetic diagnosis.