Collecting touch DNA from glass surfaces using different sampling solutions and volumes: Immediate and storage effects on genetic STR analysis

Touch DNA has become increasingly important evidence in todays' forensic casework. However, due to its invisible nature and typically minute amounts of DNA, the collection of biological material from touched objects remains a particular challenge that underscores the importance of the best collection methods for maximum recovery efficiency. So far, swabs moistened with water are often utilized in forensic crime scene investigations for touch DNA sampling, even though an aqueous solution provokes osmosis, endangering the cell's integrity. The aim of the research presented here was to systematically determine whether DNA recovery from touched glass items can be significantly increased by varying swabbing solutions and volumes compared with water-moistened swabs and dry swabbing. A second objective was to investigate the possible effects of storage of swab solutions prior to genetic analysis on DNA yield and profile quality when stored for 3 and 12 months, as is often the case with crime scene samples. Overall, the results indicate that adapting volumes of the sampling solutions had no significant effect on DNA yield, while the detergent-based solutions performed better than water and dry removal, with the SDS reagent yielding statistically significant results. Further, stored samples showed an increase in degradation indices for all solutions tested, but no deterioration in DNA content and profile quality, allowing for unrestricted processing of touch DNA samples stored for at least 12 months. One further finding was a strong intraindividual change in DNA amounts observed over the 23 deposition days which may be related to the donor's menstrual cycle.     

Palynological Investigation of Mummified Human Remains

Pollen analysis was applied to a mummified homicide victim in Nebraska, U.S.A., to determine the location of death. A control sample showed the normal ambient pollen in the garage crime scene. Ambient windborne types, common in the air of the region, dominated the control. Internal samples were analyzed from the sacrum, intestine, and diaphragm. Microfossils were recovered from the rehydrated intestine lumen. The intestinal sample was dominated by Brassica (broccoli). The sacrum sample was high in dietary types but with a showing of ambient types. The pollen from the diaphragm was dominated by ambient pollen similar to the control samples. The discovery of diverse pollen spectra from within a single mummy was unexpected. They show that ingested and inhaled pollen mixed in the corpse. The data linked the decedent to a specific crime scene in her Nebraska home in the southern tier of eastern counties on the border with Kansas.     

Do the Mental Health Consequences of Sexual Victimization Differ Between Males and Females? A General Strain Theory Approach

Sexual assault is a traumatizing event linked to numerous mental health consequences. Although extant research has thoroughly examined the collateral consequences experienced by females, limited literature exists regarding how sexual violence comparatively affects males and females. Using data from the National Violence Against Women Survey (1995–1996) and a strain theoretical framework, the current study addresses this research gap to understand the effects of sexual assault and sex on depression. Results indicate that victims of sexual assault have higher depression scores than persons who have not been sexually assaulted in their lifetime. Moreover, we found no sex effect for sexual assault on depression. The methodology used eliminated a bias typically associated with regression analyses, caused from statistical inference after improper model selection. Policy implications and directions for future research are discussed.     

Comparison of the Plexor HY System, Quantifiler and Quantifiler Duo kits using the Roche LightCycler 480 System and the ABI 7900 real time PCR instrument

In this study, we used two real time PCR platforms (Roche LightCycler 480 System and the ABI 7900 real time PCR instrument) to compare three commercial kits for DNA quantification. Special emphasis was put on PCR efficiency, detection limit and detection range. Furthermore, we tested the influence of the calibrator DNA included in the different kits on the absolute values. 40 artificial stain samples as well as 40 reference saliva samples were tested and compared. Two main observations could be made: the kits had a strong influence on the amount of DNA determined (Quantifiler^® < Quantifiler Duo^® < Plexor^® kit) whereas the real time PCR platforms showed no significant influence on the outcome.     

Rural Identity in the Twenty‐first Century: A Community of Crofters or Crofting Communities?

Crofting has been subjected to a specific regulatory regime in Scots law since the introduction of the Crofters' Holdings (Scotland) Act in 1886. The Act, introduced in response to the devastation caused by the Highland clearances, provided for security of tenure and associated rights and now provides for full ownership on both individual and collective bases. Consequently, the legal framework incorporates two distinct forms of crofting identity: one based on the crofting function and the other on a broader, place‐based definition incorporating all who live within crofting communities. Used as a basis for policymaking, each definition gives rise to different outcomes. In this paper, we use empirical research to show how such distinctions are manifested in specific conflicts of interest within contemporary crofting communities. This analysis is intended to inform the current debate surrounding the future of crofting as well as to contribute to the wider literature on the use of identity in the process of resource allocation.     

Gender-Based Violence Prevention Workshops: An Experimental Evaluation of Efficacy

Gender-based violence has become an increasingly visible and salient public problem. In the Fall of 2015, the University of Connecticut’s Violence Against Women Prevention Program (VAWPP) implemented interactive workshops in first-year experience (FYE) classes to reduce rape myth acceptance, improve knowledge and understanding of the University’s affirmative consent policy, and increase confidence in interpreting cues related to sexual consent. This randomized experimental design tested whether these outcomes were realized as a result of the workshops. In almost all instances, the workshops substantially and statistically significantly decreased students’ rape myth acceptance, increased their knowledge and understanding of consent, and improved their confidence in cue interpretation.     

Foreign direct investment and repression: An analysis across industry sectors

The impact of foreign direct investment (FDI) on repression in developing nations is still disputed. Some argue that FDI improves economic development and exports human rights values. Others criticize the exploitation of cheap labor and resources, which may lead to tensions and government oppression. Previous studies have employed aggregate FDI data with conflicting results. Alternatively, I propose that the effects depend on what kind of FDI enters a country. I build a sectoral framework to discuss how skills and technology levels, as well as the motivation for FDI, can mediate the impact. I then examine the link in a panel data analysis (1983–2010) in 121 countries, integrating sectoral FDI in several resource, manufacturing, and service industries. The results show that investment in high-skilled and high-tech sectors has positive effects. The results are robust across several measures for repression, and when accounting for sector size, regional and time effects.     

In the Aftermath of Earth,Wind, and Fire: Natural Disasters and Respect for Women’s Rights

Though much research has been devoted to a range of socioeconomic and political consequences of natural disasters, little is known about the possible gendered effects of disasters beyond the well-documented immediate effects on women’s physical well-being. This paper explores the extent to which natural disasters affect women’s economic and political rights in disaster-hit countries. We postulate that natural disasters are likely to contribute to the rise of systematic gendered discrimination by impairing state capacity for rights protection as well as instigating economic and political instability conducive to women’s rights violations. To substantiate the theoretical claims, we combine data on women’s economic and political rights with data on nine different natural disaster events—droughts, earthquakes, epidemics, extreme temperatures, floods, slides, volcanic eruptions, windstorms, and wildfires. Results from the data analysis for the years 1990–2011 suggest that natural disasters have a detrimental effect on the level of respect for both women’s economic and political rights. One major policy implication of our findings is that disasters could be detrimental to women’s status beyond the immediate effects on their personal livelihoods, and thus, policymakers, relief organizations, and donors should develop strategies to prevent gendered discrimination in the economy and political sphere in the affected countries.     

Parliamentary ethics regulation and trust in European democracies

This article presents a three-dimensional conceptualisation of conflict of interest (COI) regulation directed towards assuring the impartial and unbiased decision-making of parliamentarians. It distinguishes and separately measures (based on a new dataset) COI strictness, sanctions and transparency and shows that they indeed constitute empirically separate dimensions of parliamentary ethics regimes adopted in European democracies. In order to illustrate the usefulness of these indices, the article then examines the relationship between the three indices and trust in national parliaments across 25 democracies. Unlike the Sanctions and Transparency indices, the COI Strictness Index (composed of strictness of rules and enforcement) has a significant and robust negative association with trust, which highlights the importance of disentangling different elements of COI regimes. While future research has to explore the causal relationships between COI regulation and trust, capturing the complexity of COI regimes in an unbiased fashion and thereby making them comparable across European democracies is an essential step towards doing so.     

The Effects of a Housing Mobility Experiment on Participants’ Residential Environments

We used the Moving to Opportunity (MTO) housing experiment to inform how Housing Choice Vouchers and housing mobility policies can assist families living in high-poverty areas to make opportunity moves to higher quality neighborhoods, across a wide range of neighborhood attributes. We compared the neighborhood attainment of the three randomly assigned MTO treatment groups (low-poverty voucher, Section 8 voucher, control group) at 1997 and 2002 locations (4–7 years after baseline), using survey reports, and by linking residential histories to numerous different administrative and population-based data sets. Compared with controls, families in low-poverty and Section 8 groups experienced substantial improvements in neighborhood conditions across diverse measures, including economic conditions, social systems (e.g., collective efficacy), physical features of the environment (e.g., tree cover) and health outcomes. The low-poverty voucher group, moreover, achieved better neighborhood attainment compared with Section 8. Treatment effects were largest for New York, New York, and Los Angeles, California. We discuss the implications of our findings for expanding affordable housing policy.