Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

In this report, we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle, and to provide information for follow-up studies on neutrophils to estimate wound age. Rat injury model was established, and skeletal muscle samples were obtained from the control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as at 1, 3, 5, and 15 d post-injury (n = 5 per group). The expression of nuclei and neutrophils was detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. A total of 20 injury site areas of 0.25 mm² (0.5 mm × 0.5 mm) were then randomly selected at all time points. A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative numbers. Knowledge of five professional medical workers were considered the gold standard to measure the false positive rate (FPR), false negative rate (FNR), sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) curves. As a result, with a staining area of neutrophils from 8 µm² to 15 µm², the FPR was 4.28%–12.14%, the FNR was 12.42%–64.08%, the sensitivity was 35.92%–87.58%, the specificity was 87.86%–95.72%, the Youden index was 0.316–0.754, the accuracy was 82.80%–88.30%, and the AUC was 0.771–0.826. The AUC was largest when the cut-off value of the staining area was 12 µm². Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil infiltration. Based on the sensitivity and specificity, neutrophils can be accurately identified during regeneration of contused skeletal muscle. The TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further study. Furthermore, it will provide evidence for forensic pathology cases.     

“无阶级话语的阶级成长”:问题及其解决路径——一种基于中国工人的阶级诉求和政治期待的政治哲学

近年米工人权利意识的觉醒和一系列维护和争取权益的集体行动,标志着中国工人已经突破“原子化”、“碎片化”学术话语和政治话语的束缚,正在成为一种型构社会政治发展的阶级政治主体力量.遮蔽工人的“阶级”存在,已积聚了巨大的政治风险,其直接政治后果是消解了工人作为“阶级”的政治实践意义,甚至将工人的合理诉求和正当主张视为“反体制的力量”.如何以积极的政治姿态面对工人的“阶级”成长,主动自觉地把工人的政治诉求纳人民主政治建设的发展轨道,成为执政党和政府面对的重大问题和责任.     

灾害管理的政治理论建构

灾害与政治间的联系已逐渐受到关注,而关于二者之间关系的理论构建尚缺乏系统性.受到灾害认知理念双向互动模式的影响,政治—行政研究领域逐步展开对灾害的政治学视域的研究.从灾害管理的政治理论视角、灾害管理的政治研究的学科身份及议题避责政治、府际政治、批示政治以及框架竞争政治可被视为中国灾害管理政治研究的核心议题.     

智能制造背景下高职特色专业技能人才培养研究

全球制造业正处在以智能制造为核心的新一轮科技革命和产业变革中。我国制造业不断推进智能升级,导致新兴职业岗位的出现和职业岗位内涵的变化。高职院校特色专业建设应遵循职业教育的基本规律,创新符合产业结构调整升级的职业教育体系,培养与之相配套的高素质技术技能人才。     

试论党委书记的素质建设与管理风格养成

国有企业党委书记的素质关系着党的路线方针政策在单位的贯彻、落实和实施。一名优秀称职的党委书记应在境界、能力、态度、绩效、关键时刻的表现、抓班子带队伍的能力等六方面不断熔炼和提升个人素质,形成紧扣中心工作、务实重效、可计量与可度量、形成合力、形成"品牌"的管理风格,以最佳的状态履职尽责,引领企业的发展。     

Socioeconomic analysis: A case of Tharparkar

Tharparkar is one of the arid zones of Sindh province which is highly affected due to its drought nature and the changes in climate. These changes are posing the serious threats to the life and livelihood of the surrounding community. This study aims to explore the social and economic conditions of the Tharparkar and the role of government and social enterprises and nongovernmental organizations (NGOs) to make the livelihood and socioeconomic conditions of the Tharparkar better and comfortable. The data was collected from the current literature, visits to the studied population, and interviews from the common people and some officials of government as well as social enterprises and NGOs.     

构建110信息中心之我见

信息警务将是21世纪的警务模式 ,作为公安机关快速反应机制的龙头———110指挥中心应该做到全天候、全方位地为一线民警提供实时的警务信息支持。为此 ,要在公安机关内建立与完善以110指挥中心为龙头的信息中心 ,从而达到快速反应之目的 ,实现对社会面的动态管理。     

让平安与富裕同行——江苏省社会治安综合治理工作十年回眸

近十年来 ,江苏省狠抓“严打”和重点整治 ,坚持“打防结合 ,预防为主”,大力加强基层基础工作 ,社会治安综合治理工作不断深化 ,在“责任制”、“综合”、“群众参与”、“严打”、“基层基础工作”、“安全创建活动”等方面积累了许多宝贵的经验。     

新民主主义革命时期根据地监所制度的建立与发展简介

我国新民主主义革命时期根据地监所的建立和发展 ,是指从 1 92 7年 4月至1 94 9年 1 0月新中国成立 2 0多年来革命根据地人民民主政权看守所和监狱的建立与发展。这一时期监所制度的发展可分为以下三个阶段 :一、第二次国内革命战争时期为开创阶段 ,创建了革命政权的监所 ,提出了用共产主义思想和生产劳动对犯人进行教育、感化和改造的狱政思想 ,确立了革命根据地新型监所制度建设的方向。二、抗日战争时期为建设阶段 ,在不断总结监所工作实践经验的基础上 ,逐步形成了新民主主义的狱政思想和狱政方针以及革命监所体系。三、解放战争时期是发展阶段 ,在继承和发扬抗日根据地狱政建设经验的基础上 ,有了两次大的发展 ,使民主政权监所制度的建设向着系统化、正规化方向迈进。     

建构城市管理市场机械模式的探讨—深圳市宝安区城市管理机构改革若干问题思

借鉴西方国家政府行政管理改革的成功经验,提出调整政府的城管职能,实行政事分开,引入竞争机制,加快社会化改革是推进城市管理体制改革的关键.