A validated SPME-GC-MS method for simultaneous quantification of club drugs in human urine

A solid-phase microextraction-gas chromatographic-mass spectrometric (SPME-GC-MS) method has been developed and validated for measuring four club drugs in human urine. These drugs include gamma-hydroxybutyrate (GHB), ketamine (KET), methamphetamine (MAMP), and methylenedioxymethamphetamine (MDMA). These drugs are referred to as 'club drugs' because of their prevalence at parties and raves. Deuterium labeled internal standards for each of the four drugs was included in the assay to aid in quantitation. The drugs were spiked into human urine and derivatized using pyridine and hexylchloroformate to make them suitable for GC-MS analysis. The SPME conditions of extraction time/temperature and desorption time/temperature were optimized to yield the highest peak area for each of the four drugs. The final SPME parameters included a 90 degrees C extraction for 20min with a 1min desorption in the GC injector at 225 degrees C using a splitless injection. All SPME work was done using a 100microm PDMS fiber by Supelco. The ratio of pyridine to hexylchloroformate for derivatization was also optimized. The GC separation was carried out on a VF-5ht column by Varian (30m, 0.25mm i.d., 0.10microm film thickness) using a temperature program of 150-270 degrees C at 10 degrees C/min. The instrument used was a ThermoFinnigan Trace GC-Polaris Q interfaced with a LEAP CombiPal autosampler. The data was collected by using extracted ion chromatograms of marker m/z values for each drug from the total ion chromatograms (TIC) (full scan mode). Calibration curves with R(2)>0.99 were generated each day using the peak area ratios (peak area drug/peak area internal standard) versus concentration. The validated method resulted in intra-day and inter-day precision (% R.S.D.) of less than 15% and a % error of less than 15% for four concentrations in the range of 0.05-20microg/mL (MAMP) and 0.10-20microg/mL (GHB, KET, and MDMA). This method has the advantage of an easy sample preparation with acceptable accuracy and precision for the simultaneous quantification of these four drugs of abuse and shows no interference from the urine matrix.     

The persistence of early childhood physical aggression: Examining maternal delinquency and offending,mental health,and cultural differences

Purpose

To examine the persistence of physical aggression in preschoolers and associated correlates (i.e., socio-demographic, socioeconomic, criminality, parenting practices, maternal mental health).

Methods

One-year follow-ups are completed with 240 mothers and their preschool children (boys and girls) from the Vancouver Longitudinal Study on the Psychosocial Development of Children. A series of structural equation models are examined.

Results

Maternal psychological symptoms, juvenile delinquency, and adult offending are associated with higher levels of physical aggression in their offspring. Children of non-Caucasian mothers and those born outside of North are less physically aggressive. Cultural differences in the correlates of physical aggression were identified.

Conclusions

Maternal past delinquency, current adult offending, and mental health are important factors in the development of children’s physical aggression. The findings suggest that there are multiple pathways leading to chronic physical aggression, which may be culturally-based. Cultural differences should be taken into account when developing programs and intervening with families of children with behavioral problems.     

Institutional Abuse: A Long History

ABSTRACT

This article explores the long history of institutions for children in Australia and of the existence of abuse within them. By examining the function that such institutions were designed to perform, and the forms and structures that were devised to best achieve such purposes, the article argues that abuse was all too often not simply inherent in, but essential to, institutional operation. It pays particular attention to the classification of children deemed to be in need of institutional “care” and shows how, through a process of “othering”, their institutionalisation too often rendered them vulnerable to abuse.     

Self-Reported Communication Variables and Dating Violence: Using Gottman’s Marital Communication Conceptualization

Communication behaviors, while extensively studied within the marital field, have received only peripheral attention in violent dating relationships. The purpose of this research was to better establish empirical continuity between the marital and dating literatures by exploring communication variables that have been identified in marital relationships broadly and their self-reported manifestation in violent dating relationships. Using Gottman’s (1999) marital communication conceptualization, individuals were assessed on adaptive and maladaptive communication variables and relationship aggression. Results suggested that negative communication behaviors were associated with, and predicted, aggression in participants’ dating relationships, consistent with findings from the marital literature. However, repair attempts, generally considered an adaptive communication behavior, predicted aggression victimization. Implications and how these data fit within the context of recent research on positive marital communication behaviors are explored.     

The clinician–patient working alliance: is it a significant predictor of psychiatric medication adherence in a sample of recently released parolees?

Persistent psychiatric symptoms can serve as a major barrier to the successful reintegration of parolees with mental illness. Thus, it is important to identify factors that might impact their mental health recovery, such as low adherence to their treatment regimen. The strength of the clinician/patient working alliance has been found to be significantly associated with psychiatric medication adherence in prior research, but this relationship has not been assessed in a parolee population. Logistic regression analysis was used to determine if patient-rated working alliance was a significant predictor of low psychiatric medication adherence while taking into account alcohol/illegal drug use, age, and ethnicity in a sample of recently released parolees with mental illness (N = 49). Patient-rated working alliance, age, and ethnicity were not significant predictors of low adherence. Alcohol/illegal drug use during the follow-up period was associated with a significantly increased likelihood of meeting the criteria for low medication adherence (OR = 8.36; 95% CI = 1.60, 43.66). While working alliance was not found to be a significant predictor of medication adherence in this study, further research is needed to examine how substance misuse impacts the clinician–patient working alliance in this population.     

Atypical Accommodations for Employees with Psychiatric Disabilities

People with psychiatric disabilities often need atypical accommodations to participate in today's workforce. Some of these accommodations, including structural and social changes in the workplace, can address biases against people with psychiatric disabilities, while others ameliorate deficits that may affect performance or interaction with others. Many courts have denied requests for such accommodations based on employers' assumptions about performance or the direct threats purportedly posed by people with psychiatric disabilities. By challenging these assumptions, which can be influenced by stigma and stereotypes, and by enforcing an employer's duty to interact regarding potential accommodations, employees with psychiatric disabilities could benefit from structural and social accommodations. Courts should consider social science research in determining which nontraditional accommodations may be reasonable and whether the employer can establish that they impose any undue hardship. Such consideration will expand opportunities for people with psychiatric disabilities in the workplace without unduly interfering with employers' interests.