Successful DNA typing of ultrafiltered urines used to detect EPO doping

Endogenous and exogenous erythropoietin (EPO) present in urine can be distinguished according to their isoelectric profiles. This methodology requires urine samples to be concentrated about 200 to 1000 times with manipulations that should remove most of the cells occurring in the original sample. In this study, we tried to obtain DNA profiles from 10 ultrafiltered urines (retentates) in order to evaluate whether a formal genetic identification was technically feasible. No nuclear DNA profiles could be established from retentates, despite 34 PCR-cycles amplifications. Contrastingly, mitochondrial DNA (mtDNA) profiles were obtained for 9 out of the 10 retentates. Apart from some particularities, retentate mtDNA profiles were all distinct and matched mtDNA profiles of corresponding reference samples.     

The polymorphism of plasminogen (PLG) by ultrathin-layer isoelectric focusing. Distribution in the Veneto population (Italy)

The distribution of plasminogen phenotypes in the population of Veneto was investigated by ultrathin-layer isoelectric focusing. In our sample (n = 1325), the three common phenotypes PLG1, PLG2, PLG2-1 and two further phenotypes PLG1-V and PLG2-V were, observed and the following frequencies calculated: PLG1 = 0.84038; PLG2 = 0.15811; PLGV = 0.00151. These gene frequencies are compared to those found in other populations. Analysis of 41 mother-child pairs was in agreement with an autosomal codominant inheritance.     

The Nature of Borders and International Conflict: Revisiting Hypotheses on Territory

This study examines the utility of moving beyond a simple "on–off" dichotomous view of contiguous land borders. For each of the 301 contiguous land borders between states in the international system, measures of ease of interaction, salience, and overall border "vitalness" have been developed using Geographical Information Systems technology. These variables are used to test two major extant lines of thought in international relations literature, as well as our proposed alternative, regarding the expected effect of the "nature" of borders on interstate behavior. We conclude not only that the "nature" of contiguous borders matters but also that the relationship between each of the three border measures and the likelihood of conflict is curvilinear concave—with both high ease of interaction and high salience related to lower levels of conflict, and the middle range of both variables related to higher levels of conflict.