论中国环境公益诉讼制度之原告选择

环境公共利益主体和内容的不明确,加剧了实践中对环境公共利益受损不能实施有效救济的困境,建立环境公益诉讼制度是对环境公共利益受损实施救济的必要条件。建立环境公益诉讼制度的最核心问题是明确环境公益诉讼的原告主体范围。应确立限定性二元原告主体资格机制。环保NGO是环境公益诉讼的最适格原告。应从法律制度设置和运作模式上保障环保NGO参与环境公益诉讼的最优资格。     

中国韩国学研究的现状、特征与趋势——基于1998～2010年CSSCI数据

当前,韩国学研究已成为国内重要国别研究领域。本文运用文献计量学的方法,对1998～2010年间CSSCI数据库收录的韩国学论文数量与被引频次数量、合作论文比重与性质、作者机构分布、发文前10个单位分布、第一作者所属地区分布、发文期刊数量及载文量前10个期刊分布等进行统计,从各学科论文、作者及期刊三个方面解析国内韩国学研究现状与特征,把握其发展趋势,为国内韩国学研究的发展提供借鉴,推动国内韩国学研究更好地服务于我国经济社会发展。     

Sensitive determination of barbiturates in biological matrix by capillary electrophoresis using online large-volume sample stacking

In China, some forensic cases are caused by barbiturates. Thus, the determination of trace level barbiturates in body fluid is important for the poisoning investigation. In this study, an online large-volume sample stacking (LVSS) with polarity switching in capillary electrophoresis (CE) was applied for the sensitive determination of barbiturates. This technique involves injecting a large volume of sample into a capillary and removing the sample matrix plug out of the capillary by reversing the polarity. Quantitation limit obtained was 0.048, 0.057, 0.039, and 0.015 μg/mL for secobarbital, amobarbital, barbital, and phenobarbital (signal-to-noise ratio = 9). By using LVSS, the stacking was simply achieved at 171.7-, 169.7-, 202.7-, and 169.1-fold for the above four barbiturates. The relative standard deviation values of intraday and interday were <2.11% and 4.69%, respectively. Recoveries were ranged from 83.7 to 105.2%. Finally, the trace analysis method was applied to the analysis of real forensic specimens and has achieved satisfactory results.     

Forensic Identification Using a Multiplex Assay of 47 SNPs*

Abstract: As a powerful alternative to short tandem repeat (STR) profiling, we have developed a novel panel of 47 single nucleotide polymorphisms (SNPs) for DNA profiling and ABO genotyping. We selected 42 of the 47 SNPs from a panel of 86 markers that were previously validated as universal individual identification markers and identified five additional SNPs including one gender marker and four ABO loci. Match probability of the 42 validated SNPs was found to be 9.5 × 10^?18 in Han Chinese. SNP analysis correctly assessed a panel of historical cases, including both paternity identifications in trios and individual identifications. In addition, while STR profiling of degraded DNA provided information for 11 loci of 16 potential markers with low peak intensities, SNPstream^® genotyping was sufficient to identify all 47 SNPs. In summary, SNP analysis is equally effective as STR profiling, but appears more suited for individual identification than STR profiling in cases where DNA may be degraded.     

Dental age estimation from the developmental stage of the third molars in western Chinese population

The purpose of this study is to provide reference data about estimating dental age from third molars of the western Chinese population for comparing with other populations and being applied to the age estimation of western Chinese juveniles and adolescents. A total of 2078 digital panoramic radiographs of 989 male and 1089 female Chinese subjects aged between 5 and 23 years were examined. The mineralization status of the third molars was assessed using the formation stages described by Demirjian et al. with two modifications. The results showed that the development of third molars in the western Chinese population was likely to begin at age 5 in both males and females. The third molars 28 and 48 showed significantly higher frequency in females than in males. The third molars 18 in the stage 1, 38 in the stages 1, A and G, and 48 in the stage H showed significantly older average age in females than in males. The Demirjian's stages C and D could be used as a reference stage to determine dichotomously whether a western Chinese is more likely to be under or above age 14 or 16, respectively. This study provided reference data for the age estimation of western Chinese juveniles and adolescents by the mineralization stages of the third molar. Apart from forensic age determination in living subjects, the presented reference data can also be used for age estimations of unidentified corpses and skeletons.     

社会管理创新视角下的食品安全管理

近几年来,各类食品安全事件发生频繁,食品安全问题更是层出不穷。如何确保老百姓的食品安全,已成为政府创新社会管理中必须解决的一个重要民生问题。本文从社会管理创新角度客观分析我国现阶段食品安全管理中存在的主要问题,进而提出加强食品安全管理的建议。     

美国核电安全与法律规制

日本强震引发的核污染事件对全球核电安全管理敲响了警钟。美国是世界的核电大国,多年来运行着一套全面、协调、成熟的法律规制体系,其系统化的法律法规、易于操作的法律制度与权威高效的职能主管部门,在保障全美核电安全发展进程中发挥了重要作用;其运行法律规制体系与经验做法对我国核电安全发展也是很值得借鉴与参考的。     

以科学发展观为指导深化高校后勤社会化改革

学习贯彻落实科学发展观,加快推进高校的改革发展,必须继续深化后勤社会化改革.沈阳师范大学抓住发展机遇,坚持以"学生为本",构建学生生活区新模式,创建后勤服务新机制,对高校如何进一步深化后勤社会化改革进行了有益的探索.     

血痕特异性mRNA标记研究

血痕是案发现场尤其是命案中比较常见的生物物证,而血痕的正确组织来源推断是当前鉴定工作中急需解决的问题。随着法医物证学的不断发展,以mRNA(messenger RNA)为基础的体液斑迹组织来源鉴定技术作为一种不同于传统血痕免疫学检测的新型方法,已经越加显示其独特的优越性。在该技术的基础上,实现共同提取生物物证的RNA与DNA的目标,建立体液斑迹鉴定与DNA分型兼容的方法,有利于现场重建,提高生物物证的证据效力,完善证据链。本研究建立了一个包括血痕总RNA提取、逆转录、荧光特异引物扩增、遗传分析仪电泳检测分析等步骤的血痕来源推断技术平台。实验共采集制备了40份的中国人群(女性)外周血、16份月经血样本,筛选了5个外周血标记:HBA、HBB、GYPA、SPTB、ALAS2,2个月经血标记:MMP7、MMP11,构建了一个囊括外周血、月经血特异标记的荧光复合扩增体系。结果显示mRNA技术为基础的鉴定血痕来源的方法是可行的,并且建立了血痕RNA检验的遗传分析仪结果判读方法。     

我国证券公司的融资制度研究

证券公司的融资问题一直困扰着我国管理层,也制约着证券公司的进一步发展,进而影响着我国证券市场的发展壮大。在分析证券公司现状和考察世界各地证券公司融资制度的两大模式的基础之上,认为应建立专业化的证券融资公司以适应我国证券公司发展的需要,并就此提出了若干建议。