猪白细胞介素21基因的原核表达与多抗血清的制备

为了制备猪白细胞介素21(porcine interleukin-21,pIL-21)的多克隆抗体,采用RT-PCR从猪外周血单个核细胞(PBMC)扩增获得pIL-21基因,然后将其克隆至大肠杆菌表达载体pET-32a(+)之中,转化至大肠杆菌BL21(DE3),用IPTG诱导表达。SDS-PAGE和Western-blot分析结果显示,pIL-21以可溶性蛋白形式表达,分子质量为35ku,具有良好的反应原性。经过Ni-NTA镍离子亲和层析纯化,获得纯化的重组蛋白pIL-21。用其免疫BALB/c小鼠制备抗pIL-21血清抗体。Western-blot分析结果显示,该抗体可与杆状病毒表达的重组Cap-pIL-21、ConA和PHA共刺激的猪PBMC细胞发生特异性反应,ELISA抗体效价可达1∶12 800。上述研究结果为pIL-21的生物学功能研究奠定了重要基础。     

Analysis of Forensic Autopsy in 120 Cases of Medical Disputes Among Different Levels of Institutional Settings

Despite advances in medical science, the causes of death can sometimes only be determined by pathologists after a complete autopsy. Few studies have investigated the importance of forensic autopsy in medically disputed cases among different levels of institutional settings. Our study aimed to analyze forensic autopsy in 120 cases of medical disputes among five levels of institutional settings between 2001 and 2012 in Wenzhou, China. The results showed an overall concordance rate of 55%. Of the 39% of clinically missed diagnosis, cardiovascular pathology comprises 55.32%, while respiratory pathology accounts for the remaining 44. 68%. Factors that increase the likelihood of missed diagnoses were private clinics, community settings, and county hospitals. These results support that autopsy remains an important tool in establishing causes of death in medically disputed case, which may directly determine or exclude the fault of medical care and therefore in helping in resolving these cases.     

Thinking on the Theory of "China’s Century"

As China’s economy and global influence increase,its international relations are quickly changing.As more credence is given to predictions of a"China Century"to follow the"American Century,"interest is also increasing in the adjustments China is making to its strategic diplomacy as it prepares to take the first chair from the United States.The conceptual innovation in China’s diplomacy in 2014 is a significant harbinger in the eyes of many as to how this China Century will shape up.While China’s new diplomacy is praised,opinion differs on its future intentions.After a series of proposals were put forward at summit meetings of CICA(Conference on Interaction and Confidence-Building Measures in     

地方政府绩效评估的政治理性和技术理性——以珠海市万人评议政府为例

万人评议政府是中国地方政府绩效评估模式的一种,国内学者研究万人评议政府的主要视角是公民参与和民主政治.在运用政府绩效管理的过程逻辑对珠海市万人评议政府进行结构式描述的基础之上,我们提出了研究假设,即决策者在万人评议政府过程中作出了理性选择.然后,我们以历史性个案研究方法为基本研究方法,根据分析决策理性的理论框架和变量运用收集到的资料进行了实证检验,检验的结果证实了我们的研究假设.     

思想政治工作中的人文关怀、心理疏导方式与途径作用研究

面对急剧的社会变迁引发的心理和思想问题逐渐增多,当代思想政治工作面临着新的挑战.加强和改进思想政治工作,注重人文关怀和心理疏导,必须研究并把握好目标指向、教育内容、工作模式和实现途径.     

孔姓人群Y染色体遗传多态性研究及其法医学意义

目的获取孔姓人群Y-SNP和Y-STR遗传标记的信息,探索姓氏与Y染色体的关联性及法医学意义。方法采用等级复合扩增和SNaPshot技术检测255例孔姓男性和330例随机无关男性样本的12个Y-SNPs位点信息;采用Y filer试剂盒检测孔姓人群的17个Y-STRs基因座;应用Arlequin 3.5.1.2、Network4.6.1.1进行统计分析。结果12个Y-SNPs位点构成13种单倍群分型,孔姓人群和无关人群中最高分布频率的单倍群均为O3a2c1a-M117(21.57%,14.85%)。孔姓人群17个Y-STRs基因座构成的196种单倍型,多态性为0.993 9,单倍型14-12-25-28-19-15-12-19-12-11-12-22-12-11-14-10-19出现15次。O3-M122单倍群的中介网络树及不配对差异分析显示孔姓人群有两个中心星型分布,核苷酸不配对曲线呈单峰泊松分布。结论联合Y-SNP和Y-STR遗传标记分析表明孔姓人群存在复杂的基因交流,有多个姓氏起源,且在历史上经历过一定的扩张或持续增长,结合孔姓家谱历史分析其人群结构的遗传差异在法医学方面有潜在的应用价值。     

交通事故归责原则与损害赔偿理论研究

《道路交通事故处理办法》与《中华人民共和国民法通则》关于道路交通事故的归则原则完全不同 ,这导致了交通事故损害赔偿的不同。分析比较了各主要国家对交通事故的归责原则 ,并且从法学理论上对之进行研究。认为汽车与非机动车、行人发生的交通事故 ,应采取无过错责任原则 ,才更能体现法的公平与正义     

An Enduring Relationship

In January,the diplomatic relations between China and France mark their 55th anniversary.The history and experience accumulated in the development of the ties, including their mainstream achievements and the difficulties that have been resolved, create a good foundation for better development of the relationship in the future.     

企业政治结构与劳动治理——基于社会学视角的讨论

企业行为是嵌入在制度环境下展开的.企业政治是企业行为主体追求利益的重要手段,分为外部政治和内部政治两个层面,前者是指企业生存与发展的外部生态环境,主要表现为政企关系;后者是指企业组织内部的权力关系结构,主要表现为劳资及劳工之间的关系结构.转型期的中国企业政治发生了双重转向:从改革初期资本与国家的双向亲和关系逐渐转向了劳...