关于公安教育培训工作本质属性的思考

本文根据十八大精神的要求,从公安工作的使命和任务出发,运用历史逻辑和现实逻辑相结合的考察方法,对公安教育培训工作的本质属性进行了深入探讨.提出了公安教育培训是职业教育基础上的素质教育的观点.这对于认识和指导公安教育培训工作具有一定意义.     

论卧底警察权益保障机制之构建

随着有组织犯罪活动日益猖獗,传统侦查措施已难以与之抗衡。而卧底侦查这一隐蔽型侦查措施,逐渐成为了打击此类犯罪的一把利刃,卧底警察更是被誉为"刀尖上的舞者"。然而,在危险系数颇高的卧底任务中,卧底警察的权益保障几乎处于真空状态。因此,有必要通过完善卧底警察选拔机制、卧底警察培训机制、卧底警察心理调试机制、卧底警察身份保密机制、卧底警察岗位预留机制、卧底警察奖励与补偿机制等方式,来构建一套科学合理的卧底警察权益保障机制。     

社会主义核心价值观融入高校学生党员教育路径研究

高校是用社会主义核心价值体系武装青年的重要思想阵地。在学生党员教育培训、社会实践、行为规范、校园文化和激励考核中融入社会主义核心价值观,引导他们对核心价值观的真正认知、认同与践行,并在学习生活中发挥先锋模范作用,有利于高校学生党建工作发展方向的准确把握、高校学生党员思想政治教育的内容创新和高校学生党员健全政治人格的稳定形成。     

自媒体时代官媒重拾话语权之诉求

随着自媒体时代的到来,所谓的"互联网狂欢"改变了信息传播的固定模式。话语权即控制舆论的权力,谁掌握了话语权,谁就掌握了社会舆论走向。作为党和政府的"喉舌"的官媒,在与新兴媒体抢占舆论高地的博弈中,由于政府公信力下降导致官媒话语被"习惯性质疑",在信息的即时性、交互性日益增强情境下,官媒试图重构权力话语体系,重拾话语权,以期走出正面临的塔西佗陷阱。     

试论监管立法制度的完善——以商业特许经营备案为例

全球金融危机的发生,使得政府监管成为各界关注的热点话题,本文以特许经营备案制度为例,指出了特许备案制度实施中所面临的问题,即特许备棠的效率和效果均不理想.在借鉴国外有关立法实践和理论的基础上,提出了完善我国监管立法制度的建议:包括运用监管影响分析、提高监管透明度、运用监管替代措施等.     

ABO genotyping by duplex amplification and oligonucleotide arrays assay

Objective

Research on the application feasibility of ABO genotyping for forensic identification by oligonucleotide arrays assay.

Methods

Oligonucleotide microarrays which detect three different SNPs in exon 6 and exon 7 for ABO genotyping were used. After hybridization wash, the arrays were scanned and fluorescence intensities were analyzed using microarray population studies on ABO was carried out in a sample of 115 unrelated Chinese Han individuals oligonucleotide arrays for genotype detection. The method was also applied to cases.

Results

Technique could identify six genotypes of ABO system and the results of GeneChip analyses confirmed by PCR–RFLP. According to the results of population studies, no significant deviations Hardy–Weinberg equilibrium could be found. The observed heterozygosity (H-obs) was 0.591. Expected heterozygosity (H-exp) was 0.616. The polymorphic information content (PIC) was the average exclusion probability in paternity testing for duos (PE (1)) was 0.188. The average exclusion probability in paternity testing for trios (PE(2)) was 0.344. The discrimination power 0.777.

Conclusion

The data and case application demonstrated that ABO typing by oligonucleotide probe arrays was a useful technique for paternity testing and individual identification.     

电子商务环境下商标侵权行为实证分析

电子商务环境下,企业商标权的战略保护已成为企业发展急需解决的重要课题。从电子商务概念和特征出发,结合实际案例对四种商标侵权行为进行分析,归纳概括各种侵权行为方式,解析侵权行为与现行法律规定的冲突与矛盾,可以为我国企业强化知识产权意识,完善相关电子商务环境下商标侵权行为法律法规提供镜鉴。     

扩增12S rRNA基因鉴定生物检材种属

目的建立一种能够在同一反应条件下鉴定多个具体物种．又满足简单、快速、特异、灵敏、准确等实用要求的种属鉴定方法。方法从GenBank中获取人、鸡、鸭、鹅、猪、兔、鼠、绵羊、水牛、狗、山羊等11个物种的12SrRNA基因序列．设计一对针对上述11个物种的通用引物和分别针对人、鸡和鸭的特异引物,同时扩增各物种12SrRNA基因。以通用引物扩增片段为内部对照．以特异引物扩增片段用于人、鸡和鸭的种属鉴定,并分别对人、鸡、鸭单一检材以及人和鸡、人和鸭、鸡和鸭等二元混合DNA进行鉴定。结果通用引物扩增,各物种均有400bp左右的扩增片段：特异引物只对各自目标种属有扩增产物,片段大小：人163bp、鸡286bp、鸭374bp;测序结果与GenBank既有序列比对,Identities分值：人100％、鸡99％、鸭100％;通用引物扩增人、鸡和鸭检材的灵敏度为2．5Pg;特异引物扩增灵敏度人为2．5pg,鸡、鸭均为200Pg;混合DNA中任一种DNA的含量只要高于检测灵敏度即可被准确检出．不受另一种DNA量的干扰：盲测结果准确。结论本方法可以利用同一种PCR反应条件鉴定多个物种的种属。     

Identification and dating of the fountain pen ink entries on documents by ion-pairing high-performance liquid chromatography

A novel approach for the identification and dating of the fountain pen ink entries on paper has been established by ion-pairing high-performance liquid chromatography (IP-HPLC). Twelve black and six red fountain inks have been collected, and their ink entries have been prepared by drawing lines on paper. The chromatographic conditions for separation of their dye components after extraction with solvents were optimized. Under the optimized conditions, the 18 fountain pen inks were differentiated individually by comparing the number of detectable main or minor dye components, and the relative peak intensities of each component. The ink entries were artificially and naturally aged, and the analysis results showed that the ink dye components were significantly decomposed when exposed to UV or fluorescent light compare to those of inks stored under natural condition. The changes of the relative peak height for the dye components were linearly related to the aging time, especially under natural aging conditions. The degradation characteristics of the dye components under different aging conditions provide scientific evidences for dating of the suspicious fountain pen ink entries on document.     

刑事审判中的证据引出规则

“证据引出”规则是在刑事审判过程中 ,要求控辩双方通过一定的方式使一项证据材料能够被允许作为证据在法庭上出示的规则 ,其实质是在控辩双方向裁判者展示自己的证据材料之前 ,对其准备在法庭上展示的证据材料进行前置性审查 ,以排除不具有证据能力的证据材料 ,使之不能在法庭上向裁判者展示。证据引出规则对于实现实体公正、程序公正和诉讼效率等程序价值都有现实意义。