猪圆环病毒2型ORF2基因插入猪细小病毒VP2基因3'端对病毒样颗粒形成的影响

以猪细小病毒(PPV)四川分离株SC-1的VP2基因3'端为靶点,在第1215和1216位之间插入长约700 bp的猪圆环病毒2型(PCV2)ORF2基因片段,将重组后的PPV VP2-PCV2 ORF2克隆到真核表达载体pEGFP-C1中,构建了重组质粒pEGFP.VO;经酶切和PCR鉴定后,采用脂质体介导法将重组质粒pEGFP.VO转染COS-7细胞,应用倒置荧光显微镜、电镜、免疫荧光以及生物信息学技术对表达产物进行观察分析.结果显示,重组质粒pEGFP.VO在COS-7细胞中得到表达,但在电镜下没有观察到病毒样颗粒.通过对PPV衣壳蛋白的三维结构分析及免疫荧光检测发现,PCV2 ORF2基因的插入位点位于PPVVP2基因的3'端,相应表达产物则位于VP2蛋白多肽的C端,且被其包裹在内,这可能影响了病毒样颗粒的形成,表明,PPV VP2基因3'端不适合外源基因插入构建重组病毒样颗粒.     

以科学发展观为指导加强安全文化建设

如何以科学的发展观为指导,发挥路局直接管理站段新体制的优势,加强安全文化建设,强化安全管理基础,形成适应铁路跨越发展要求、富有生机活力和人文感化力的安全文化体系,是站段实现安全自我管理、自我控制、自我发展的必由之路。一、培育“安全至上”的共同价值观,为安全文化创建能源库企业安全文化就是在企业的核心价值体系基础上形成的,具有延续性的共同的认知系统和习惯行为方式。它使安全包含于文化,文化又作用于安全。这一源自文化层面的力量,比行政管理的命令、监督、惩罚所产生的力量更具有凝聚力。铁路和安全与生俱来,相伴而行,铁…     

The Subject Status of Procuratorate in Marine Litigation on "Public Interests Protection"

The litigations of marine natural resources ormarine ecological (environmental) damages involve two different types of litigations, and the procuratorate should be involved in the litigationsas different litigation subjects. The compensatory litigation of marine natural resource damageis the private interest litigation which relieves the private interest damage of state owned natural resources. According to The Administrative Litigation Law and The Supreme People's Court and the Supreme People's Procuratorate's Interpretation of Several Issues Concerning the Application of Law in Procuratorial Public Interest Litigation Cases, the procuratorate should be the supervising subject. However, the compensatory litigation of marine ecological (environmental) damage is the public interest litigation which relieves public interests, and on the basis of Marine Environment Protection Law, The Civil Procedure Law and The Supreme People’s Court and the Supreme People's Procuratorate's Interpretation of Several Issues Concerning the Application of Law in Procuratorial Public Interest Litigation Cases, the procuratorate should be the supplementary subject. Due to the particularity of the marine litigations, the procuratoratefiles lawsuits against the marine natural resources ormarine ecological (environmental) damages, which shall apply both to The Provisions of the Supreme People's Court on Several Issues Concerning Compensation for Damages of Marine Natural Resources and Ecological Environment and The Supreme People’s Court and the Supreme People's Procuratorate's Interpretation of Several Issues Concerning the Application of Law in Procuratorial Public Interest Litigation Cases.     

气-质联用法（EI．PCI．NCI）在系统毒物分析中的应用

本文应用电子轰击质谱(EI),正、负化学电离质谱(PCI.NCI)技术,建立了315种常见毒,药物的气一质联用系统分析方法。并建立了 EIMS、PCIMS、NCIMS 质谱图谱库。司法鉴定实践表明使用气相保留指数,特征碎片和 EI、PCI、NCI 图谱库检索等多指标的系统分析方法,是未知物鉴定的强有力的工具。     

关于将松北新区建成高能级发展科技创新城的调研报告

盖如垠书记到任哈尔滨后,提出了“北跃、南拓、中兴、强县”的发展战略构想,并经市委十二届七次全会丰富完善已成为哈尔滨未来发展的战略取向。通过学习市委全会精神,我们认为,这一战略构想符合哈尔滨的发展实际,十分及时地抓住了哈尔滨发展的关键和主轴,是哈尔滨经济社会实现跨越式发展的重要抉择。近期,我们班的全体学员对实施“北跃”战略——将松北新区打造成具有高能级发展功能的科技创新城问题进行了调研和思考,形成了此报告,供市委决策参考。     

吉林省参与图们江区域国际合作开发的问题与对策

以多国合作为特征的图们江区域经济合作开发项目近几年有了实质性的进展,各成员国加强了务实开发的力度。中朝“路港区”工程、中俄“路港关”工程、中蒙“两山”工程等开始启动,并有了一个较好的开端。但对于吉林省来讲,当前又遇到了对外通道未能真正顺利打开、跨国边境经济合作区没有形成完整的互动机制、市场贸易主体也未得到真正确立等问题。为此应加快推进图们江区域国际经济合作。     

Postmortem Analysis of Electrogram Records from an Implantable Cardioverter‐Defibrillator (ICD) in the Reconstruction of a Road Traffic Accident

The case of a 69‐year‐old man, equipped with an ICD and suffering from several chronic cardiac diseases, who died in a car accident, was presented. We analyzed electrogram records from the ICD explanted from the body during the autopsy, which showed that the driver had suffered from malignant ventricular arrhythmia—ventricular fibrillation (VF). A thorough analysis of the details of the accident, as well as the timing of VF and the rhythm observed after the discharge of the ICD showed that the direct cause of the accident was the episode of arrhythmia resulting in a loss of consciousness. Therefore, the presented case illustrates the usefulness of postmortem analysis of electrogram records from ICDs in the reconstruction of road traffic accidents. In such cases, if the victims are implanted with ICDs, it should be a routine procedure performed by forensic pathologists.     

Comparing the access conditions for minimum income support in four EU member states for national,EU and non-EU citizens

This research analyses the conditions imposed on national, EU and non-EU citizens who wish to access minimum income (MI) benefits within four EU Member States, specifically within Finland, France, Ireland and Spain. The primary aim is to identify and compare the required MI access conditions. Furthermore, focus is given to the residence requisites, which are discussed in relation to relevant supranational regulations in order to detect possible multilevel implications. The paper concludes with the identification of different MI conditions, such as stricter age requisites in France and Spain. Moreover, the study of national cases allows for consideration of how the EU social protection floor works at the national level. In this regard, the restrictions that affect EU/EEA migrant jobseekers and economically inactive population groups who wish to access MI in Finland, France and Ireland show the limits of the EU minimum social assistance floor, only recognised for EU/EEA migrant workers. Finally, implications arise according to human rights instruments such as the European Social Charter, which demands that social assistance shall not be confined to nationals or to certain categories of foreigners, allowing for comparison between the different personal scopes of the equal treatment principle required by the distinct supranational levels.     

Analyzing and Interpreting Lime Burials from the Spanish Civil War (1936–1939): A Case Study from La Carcavilla Cemetery

Over 500 victims of the Spanish Civil War (1936–1939) were buried in the cemetery of La Carcavilla (Palencia, Spain). White material, observed in several burials, was analyzed with Raman spectroscopy and powder XRD, and confirmed to be lime. Archaeological findings at La Carcavilla's cemetery show that the application of lime was used in an organized way, mostly associated with coffinless interments of victims of Francoist repression. In burials with a lime cast, observations made it possible to draw conclusions regarding the presence of soft tissue at the moment of deposition, the sequence of events, and the presence of clothing and other evidence. This study illustrates the importance of analyzing a burial within the depositional environment and taphonomic context.     

A novel network-based analysis to measure efficiency in science and technology parks: the ISA framework approach

In the last years, Science and Technology Parks (STPs) have been considered facilitators of inter-organizational relationships, as well as instruments of public policy, by creating networks and allowing access for the learning of local innovation. In this study, we propose a quantitative tool as support for measuring the efficiency of STPs through the analysis of the complex networks they form. We present a generic framework called the Inter-Relationship Science-Park Analysis (ISA) framework to study STPs at three different levels of management: (1) individual entities (research centres, universities, innovation companies, etc.); (2) STP global management; and (3) productive sector development. Moreover, we have applied the ISA framework to a real-world case study: the Walqa Science and Technology Park. Through it we have been able to know what relationships are established within this Science and Technology Park and which are the most important nodes in this network of interactions.