鸡减蛋综合征诊断抗原与超免疫血清的研制

将鸡减蛋综合征(EDS)种毒AV-127、AV-HS-1接种鸭胚,孵育120 h后,收取尿囊液测定其血凝(HA)效价,加体积分数0.2%甲醛灭活作为诊断抗原;将病毒对鸡、兔进行3次免疫,当抗体效价达到29以上时分离血清,制备成超免疫血清.试验证明,该EDS诊断抗原和超免疫血清具有特异性强、敏感性高等特点,可用于感染鸡群的疫情调查、免疫鸡群的抗体水平监测、EDS的预防、治疗和对减蛋综合征病毒(EDSV)进行深入研究.     

Methamphetamine-related fatalities in forensic autopsy during 5 years in the southern half of Osaka city and surrounding areas

To outline the recent features of methamphetamine-related fatalities from the medico-legal point of view, a retrospective investigation of forensic autopsy cases involving methamphetamine during a 5-year period (1994-1998) in the southern half of Osaka city and surrounding areas (about 1.57 million population) was undertaken. Among 646 autopsy cases, methamphetamine was detected in 15 victims (nine males, six females; 16-71 years of age; most frequently in males in their thirties). Primary scenes of fatal events were concentrated in the middle of the city. About half of them were transferred from emergency medical centers (survival time, up to 30 h). The cause and manner of death were: methamphetamine poisoning (n=4), homicide (n=4), accidental falls and aspiration from drug abuse (n=4), fire death (n=1), myocardial infarction (n=1), and cerebral hemorrhage (n=1) under drug influence. Usually injection scars and fresh puncture sites were found. Blood methamphetamine concentrations were 2.29-17.05 micromol/dl in the fatal poisoning, 0. 44-3.80 micromol/dl in deaths from other extrinsic causes (trauma), and 1.35-2.17 micromol/dl in cardio- and cerebrovascular strokes. Common complications were cardiomyopathy, cerebral perivasculitis and liver cirrhosis/interstitial hepatitis. Fatal and nonfatal methamphetamine poisonings are separately dealt with by the administrative medical examiner's office and in emergency medical centers. Tightly cooperative approaches of clinical and medico-legal experts are required for the effective social and medical management of drug abuse.     

乐山市二零零三年上半年经济运行情况及全年经济形势预测

2003年上半年已经过去。认真分析全市上半年经济发展状况,科学预测全年经济形势,对做好下半年的工作,圆满完成全年经济发展目标任务,显得非常重要。 一、上半年经济运行情况 上半年,我市经济运行状况继续趋好。全市国内生产总值完成85.07亿元,增长12.3％,增幅比去年同期提高1.8个百分点,高于全省平均水平1.3个百分点。其中:第一产业增加值完成13.27亿元,增长4.4％;第二产业增加值42.62％亿元,增长17.3％;第三产业增加值29.18亿元,增长9.1％。上半年的经济运行呈现以下几个特点:     

陈英士略论

陈英士历来是个备受争议资产阶级革命家。本文对其生平史实进行了进一步的考辨和评价。陈英士具有坚定的革命立场和献身精神;同时,他对民主政治的基本原则有一定的了解;他的主要历史功绩是辛亥革命期间领导上海及周边地区光复成功,在中华革命党时代指挥了一系列的反袁斗争。陈英士的主要缺点是政治气量狭窄,宗派情绪强烈,并沾染了流氓作风。陈英士的缺点后来被蒋介石领导的中国国民党继承和发扬,则是中国资产阶级软弱性在政治领域的必然结果。     

菲华商联总会“捐建农村校舍运动”述评——社会资本角度的诠释

战后初期,华菲冲突剧烈,为改善华菲关系,华社积极为主流社会提供各种公益性服务,其中,商总发起与承担的"捐建农村校舍运动"成效最为卓著.经过40多年的努力,这一工作已取得了很大成就,在华社及主流社会都获得了很高的声誉,为华社创造了丰富的社会资本,对华菲融合起到了积极的促进作用.     

Study on the application of parent-of-origin specific DNA methylation markers to forensic genetics

In paternity test, especially in motherless cases, the allele inherited from father (obligatory gene, OG) often cannot be determined. The paternity exclusion probability (PE) of a genetic marker is reduced considerably. Therefore, it is necessary to develop a new technique, by which the parental origin of alleles can be determined without genealogical analysis. In this paper, we explored the possibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles, choosing the imprinted single nucleotide polymorphism (SNP) locus rs220028 (A/G) as a model system. We typed the SNP by mutagenically separated PCR (MS-PCR). The frequencies of alleles were A = 0.5085, G = 0.4915; the unbiased heterozygosity was 0.5020. In order to discriminate between the maternal allele and paternal allele, post-digestion MS-PCR, a novel PCR based methylation analysis and SNP typing technique was developed and performed on 18 heterozygous children, and the methylated maternal allele was detected specifically. As a pilot study on the use of epigenetic markers in forensic genetics, our results demonstrated the feasibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles.