Mitochondrial DNA error prophylaxis: assessing the causes of errors in the GEP'02-03 proficiency testing trial

A qualitative and quantitative analytical method was developed and validated for the determination of 49 licit and illicit drugs in oral fluid. Small oral fluid samples, volume 1mL, were collected from volunteers using a modified Omni-Sal device and the analytes were extracted from an oral fluid/buffer mixture using a single Bond Elut Certify solid phase extraction cartridge. Liquid chromatography-tandem mass spectrometry (LC-MS-MS) and gas chromatography-repetitive full scan mass spectrometry (GC-MS) were used in parallel to analyze the extracts for the targeted drugs. Extracts were analyzed by GC-MS in their underivatized form and as their pentafluoropropionyl derivatives. Deuterated internal standards were used for quantification of drugs of abuse by LC-MS-MS to minimize matrix effects. Methadone-d(9) and tumoxetine were used as the internal standards for quantification of non-derivatized and derivatized analytes respectively by GC-MS. Linearity was demonstrated over the range 5-200 ng/mL and limits of detection were less than 4 ng/mL for each drug analyzed. The method demonstrated acceptable recoveries for most of the analytes and good intra- and inter-day precision. Acquisition of data by repetitive full scan GC-MS allows the addition of further analytes to the target menu.     

A case of commingled remains from rural South Africa

In the spring of 2003, bush cutters discovered a large grain bag filled with human skeletal remains in a forest in South Africa. Differential taphonomic processes observed on the bones suggested that the individuals had not died at the same time or had decomposed under different circumstances. The remains were sorted using gross morphological techniques which included assessing the number of skeletal elements present, visual pair matching, articulation, process of elimination and taphonomy. Standard techniques were used to estimate age, sex, racial affinity and stature. A minimum number of individuals (MNI) of 10 was established, which included 7 adult males, 1 adult female and 2 juveniles. Trauma was observed on only one individual. Approximately, 80% of the skeletal remains were missing. These various skeletal elements may have been lost by accident, normal taphonomic processes or taken by scavengers. Possible origins for these skeletal remains include removal from a formal grave, found in the veldt or murdered.     

Interpretation of postmortem change in cadavers in Spain

Estimating time since death is especially difficult in the examination of poorly preserved cadavers and depends on the experience of the examiner and comparison with previously documented cases showing similar characteristics. The present study reports on information obtained over the past ten years through the work of the Laboratorio de Antropología y Odontología Forense (LAF) of the Instituto Anatómico Forense de Madrid, Spain, in particular evaluating how the type of fracture influences postmortem change. From the original 225 forensic cases examined between 1992 and 2002 in the LAF, a sample of 29 cases were selected from various regions of the Spanish mainland. A data collection protocol was established to reflect factors which the existing specialized literature, documenting the relation existing in the sample analyzed between time since death and the extent of postmortem change, which in the environments examined are distributed into the following phases: Phase 1 (putrefaction): one week to one month on the surface and two months in water. Phase 2 (initial skeletonization): two months on the surface and five to six months in water. Phase 3 (advanced skeltonization): six months to 1.5 years on the surface and 2.5 years buried. Phase 4 (complete skeletonization): about one year on the surface and three years buried. This paper also provide useful information on the impact of carrion insect activity, location, climate, seasonality, and predator.     

Hochwasserschutz im Spannungsverhältnis zum übrigen Fachplanungsrecht, Raumordnungsrecht und zur Bauleitplanung

Durch das Oderhochwasser, aber vor allem durch die Flutkatastrophe an der Elbe im August 2002, ist der Hochwasserschutz verstärkt in das öffentliche Bewusstsein getreten. Dies hat auf der Ebene von Bund und Ländern zu verschiedenen Aktionen geführt, die sich nunmehr in Gesetzesänderungen niederschlagen sollen 1. Der Hochwasserschutz kann dabei durchaus in ein Spannungsverhältnis zu anderen öffentlichen und privaten Anliegen und Belangen treten—vor allem, wenn sich aus deren Sicht Einschränkungen für die bauliche oder sonstige Nutzung der betroffenen Flächen ergeben können. Ein wichtiger Teilausschnitt aus diesem Gesamtproblem ist das Spannungsverhältnis des Hochwasserschutzes zum übrigen Fachplanungsrecht, zum Raumordnungsrecht und zur Bauleitplanung. Hierüber soll auf der Grundlage des vorliegenden Gesetzentwurfs der Bundesregierung zum verbesserten Hochwasserschutz berichtet werden. *) Der Beitrag beruht auf einem Vortrag, den der Verfasser auf dem 9. Leipziger Umweltrechts-Symposium Rechtliche Aspekte des vorbeugenden Hochwasserschutzes am 22. 4. 2004 gehalten hat und der auch in dem von dem Direktoren des Instituts für Umwelt- und Planungsrecht, Prof. Dr. Martin Oldiges und Prof. Dr. Wolfgang Köck betreuten Tagungsband erscheinen wird.     

Fatal cranial injuries caused by an electric angle grinder

A case of fatal cranial injuries caused by an angle grinder is reported. The scalp lesions were typical of those produced by a cutting disk in a side-slipping movement. On the cranial vault were two bony losses of substance, one of which was deep enough for intracranial penetration of the disk. Signs of deflection of the disk, identical to those found on the scalp, were observed on the external bony table. Because of the circumstances in which the victim was discovered, in particular the damage to the machine which had a broken handle, and the lack of any indication of homicide or suicide, an accident is the most likely hypothesis.     

B. Reviews and notes of recent bibliographies

i. Le Moyen‐Orient contemporain. By Guy Feuer, with the collaboration of S. Chauvin and F. Pourcelet. (Guides de Recherches, No. 6) Paris, Fondation Nationale des Sciences Politiques, 1975. pp. 245. 48 fr.

ii. Eléments de bibliographie des études arabes. By Aubert Martin. Paris, Publications Orientalistes de France, 1975. pp. iii, 235. 35 fr.

iii. The Emergence of Arab Nationalism from the 19th century to 1921: A Bibliography. Compiled and annotated by Frank Clements, London, Diploma Press, 1976. pp. x, 290. £10.00

iv. Union catalogue of Arabia Serials and Newspapers in British Libraries. By the Middle East Libraries Committee, edited by Paul Auchterlonie and Yasin H. Safadi. London, Mansell, 1977. pp. xvi, 146. £12.50.

v. Arabia Historical Writing, 1973 & 1974. By Fawzi Abdulrazak. Cambridge, Mass., Harvard University Library, 1974 & 1976. 2v. $ 8.65 (for both vols).

vi. Modern Arabic Literature: A Bibliography of Articles, Books, Dissertations and Translations in English. By Salih J. Altoma. Bloomington, Indiana University, Asian Studies Research Institute, 1975. pp. 73. $ 4.00

vii. The Kurds in Iran, a Selected and Annotated Bibliography. By Wolfgang Behn. London, Mansell, and Munich, Verlag Dokumentation, Publishers, 1977. pp. 76. £6.00

ix. American Doctoral Dissertations on the Arab World 1883–1974. Second edition. By George Dimitri Selim. Washington D.C., Library of Congress, 1976. pp. xviii, 173. Obtainable from: Superintendent of Documents, Government Publications Office, Washington D.C. 20402. Price: outside USA $5.75 plus $1.44 for overseas postage.

x. International and regional politics in the Middle East and North Africa: a guide to information sources. (International Relations Information Guide Series, 6). By Ann Schulz. Detroit, Gale, 1977. pp. xii, 244. $18.00

xi. The politics of African and Middle Eastern states: an annotated bibliography. By Anne Gordon Drabek & Wilfrid Knapp. Oxford, Pergamon, 1976. pp. x, 192. £6.75     

The Use of Laser Microdissection in Forensic Sexual Assault Casework: Pros and Cons Compared to Standard Methods

Sexual assault samples are among the most frequently analyzed in a forensic laboratory. These account for almost half of all samples processed routinely, and a large portion of these cases remain unsolved. These samples often pose problems to traditional analytic methods of identification because they consist most frequently of cell mixtures from at least two contributors: the victim (usually female) and the perpetrator (usually male). In this study, we propose the use of current preliminary testing for sperm detection in order to determine the chances of success when faced with samples which can be good candidates to undergo analysis with the laser microdissection technology. Also, we used laser microdissection technology to capture fluorescently stained cells of interest differentiated by gender. Collected materials were then used for DNA genotyping with commercially available amplification kits such as Minifiler, Identifiler Plus, NGM, and Y‐Filer. Both the methodology and the quality of the results were evaluated to assess the pros and cons of laser microdissection compared with standard methods. Overall, the combination of fluorescent staining combined with the Minifiler amplification kit provided the best results for autosomal markers, whereas the Y‐Filer kit returned the expected results regardless of the used method.